Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   BJD94_RS14065 Genome accession   NZ_CP017635
Coordinates   2785084..2785674 (+) Length   196 a.a.
NCBI ID   WP_038940289.1    Uniprot ID   -
Organism   Vibrio vulnificus Env1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2780084..2790674
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BJD94_RS14050 (BJD94_12770) oxyR 2780466..2781365 (+) 900 WP_045591363.1 DNA-binding transcriptional regulator OxyR -
  BJD94_RS14055 (BJD94_12775) - 2781434..2783947 (-) 2514 WP_017419655.1 penicillin-binding protein 1A -
  BJD94_RS14060 (BJD94_12780) pilM 2784087..2785094 (+) 1008 WP_103155248.1 type IV pilus assembly protein PilM Machinery gene
  BJD94_RS14065 (BJD94_12785) pilN 2785084..2785674 (+) 591 WP_038940289.1 PilN domain-containing protein Machinery gene
  BJD94_RS14070 (BJD94_12790) pilO 2785667..2786257 (+) 591 WP_011079354.1 type 4a pilus biogenesis protein PilO Machinery gene
  BJD94_RS14075 (BJD94_12795) pilP 2786247..2786759 (+) 513 WP_038940290.1 pilus assembly protein PilP Machinery gene
  BJD94_RS14080 (BJD94_12800) pilQ 2786773..2788557 (+) 1785 WP_017419660.1 type IV pilus secretin PilQ Machinery gene
  BJD94_RS14085 (BJD94_12805) aroK 2788759..2789277 (+) 519 WP_011079357.1 shikimate kinase AroK -
  BJD94_RS14090 (BJD94_12810) aroB 2789310..2790401 (+) 1092 WP_038964151.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22658.89 Da        Isoelectric Point: 7.6441

>NTDB_id=200823 BJD94_RS14065 WP_038940289.1 2785084..2785674(+) (pilN) [Vibrio vulnificus Env1]
MQHKINLLPWREHKREEHKRRFVSLVALGALIAVGIQWGIGQYFSYQQRLQQERLDYLTHYIAQLDQRIQAMNIAKEEHS
QILARLKVVESLQAERNKTTAFMNLMTELIPEGVYVDKIKMNTNDVELAGISDSTARLATMLDNLERSSFLKEVEMHSIV
HDKKRFGKSFQTFTVSFVFDEHADSANTLAKEGNHG

Nucleotide


Download         Length: 591 bp        

>NTDB_id=200823 BJD94_RS14065 WP_038940289.1 2785084..2785674(+) (pilN) [Vibrio vulnificus Env1]
ATGCAGCATAAGATCAACCTGTTACCTTGGCGTGAACACAAGCGTGAGGAGCATAAACGTCGTTTTGTCAGTCTGGTCGC
ATTAGGTGCCTTGATCGCGGTCGGTATTCAGTGGGGTATTGGCCAGTACTTTTCATACCAACAACGTTTGCAGCAAGAGC
GTCTCGATTATCTTACCCACTATATCGCACAGTTGGATCAGCGTATTCAAGCGATGAACATTGCCAAGGAAGAGCATAGC
CAGATTCTCGCTCGACTCAAGGTTGTCGAGTCGCTTCAAGCGGAGCGCAACAAAACCACCGCATTTATGAACTTGATGAC
GGAGCTGATCCCAGAGGGGGTGTATGTCGACAAGATCAAAATGAACACCAACGACGTTGAGTTGGCAGGTATAAGCGATA
GTACCGCTCGACTCGCCACCATGTTGGATAATTTGGAACGTTCCTCCTTCCTGAAAGAAGTGGAAATGCATTCGATTGTT
CATGACAAGAAGCGTTTTGGTAAGAGCTTCCAGACGTTTACCGTCTCTTTTGTGTTTGATGAACATGCCGATTCTGCCAA
TACGTTGGCCAAGGAGGGGAATCATGGCTAG

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(102-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

67.956

92.347

0.628
  pilN Vibrio cholerae strain A1552

63.277

90.306

0.571

Multiple sequence alignment