Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   BIZ52_RS05030 Genome accession   NZ_CP017457
Coordinates   1108314..1110410 (+) Length   698 a.a.
NCBI ID   WP_027227636.1    Uniprot ID   -
Organism   Legionella pneumophila subsp. fraseri strain Detroit-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1103314..1115410
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BIZ52_RS05005 (BIZ52_04935) ravI 1104074..1105081 (-) 1008 WP_027227632.1 Dot/Icm T4SS effector RavI -
  BIZ52_RS05010 (BIZ52_04940) pilM 1105500..1106564 (+) 1065 WP_027227633.1 type IV pilus assembly protein PilM Machinery gene
  BIZ52_RS05015 (BIZ52_04945) pilN 1106568..1107116 (+) 549 WP_027227634.1 PilN domain-containing protein Machinery gene
  BIZ52_RS05020 (BIZ52_04950) pilO 1107130..1107729 (+) 600 WP_027221266.1 type 4a pilus biogenesis protein PilO Machinery gene
  BIZ52_RS05025 (BIZ52_04955) pilP 1107726..1108310 (+) 585 WP_027227635.1 pilus assembly protein PilP Machinery gene
  BIZ52_RS05030 (BIZ52_04960) pilQ 1108314..1110410 (+) 2097 WP_027227636.1 type IV pilus secretin PilQ Machinery gene
  BIZ52_RS05035 (BIZ52_04965) aroK 1110856..1111383 (+) 528 WP_010946667.1 shikimate kinase AroK -
  BIZ52_RS05040 (BIZ52_04970) aroB 1111370..1112473 (+) 1104 WP_027227637.1 3-dehydroquinate synthase -
  BIZ52_RS05045 (BIZ52_04975) - 1112476..1113930 (+) 1455 WP_027227638.1 SPOR domain-containing protein -
  BIZ52_RS05050 (BIZ52_04980) - 1114041..1114472 (+) 432 WP_027221272.1 universal stress protein -

Sequence

Protein


Download         Length: 698 a.a.        Molecular weight: 77584.92 Da        Isoelectric Point: 9.6310

>NTDB_id=198361 BIZ52_RS05030 WP_027227636.1 1108314..1110410(+) (pilQ) [Legionella pneumophila subsp. fraseri strain Detroit-1]
MRKIIAILILISASLSMAYSKDNSLISVKVLPLPENRVRIDFQFSKPLKQLPASFITQKPPRLVLDFISTDMQIPADQKT
KTIQLGSLHSYNIVAVRDRIRAILELDRSVSYSGSTAGSVYSLVLNGKSNDLFENSKEVFITNQVVNAKHEIKRIDFRGI
EKQGGRVLIDVSDTGIPIDVTQVGKEIVVNFLNTRIPLNLMKRYDVSDFHSPVQIITMQQEGKKVRMTILSKGDYGHFVY
QVNKQFMVDVFPLTAEEIRQAKLKKQVFTGKRISLNFQNISIRAVLQLLADFTGINMVVSDKVQGDITLRLNDIPWDQAL
DIILTTQGLDKRRKGNVMLIDTKASMDKMEEDQLKSQQTIEKLEPIRSDLIQINYAKAADIAVLIKDKQNSLLSDRGKIS
VDARTNTIWIQDTGTKIEEVRELIKQLDIPVKQVLIEARIVEVTKDFSQDIGIRWGVSRPTHLSGTLEGANELARGTAPA
NVVPLERRLNLDLAAAPLTGATPASVGIALAKLNDNILLDLELSALESEGRAELISSPRLITTNQQAAVIESGEEIPYQE
ATSSGATAVAFKKAVLSLKVTPQITPDGKILMDLQINQDTPSPQTFLGVPAIITKEIQTNVLVNNGQTIVLGGIYKQDKN
KVINRIPFFGELPVVGILFSNRQITLKNEELLIFITPRIITNALSITTIEGREKDVYK

Nucleotide


Download         Length: 2097 bp        

>NTDB_id=198361 BIZ52_RS05030 WP_027227636.1 1108314..1110410(+) (pilQ) [Legionella pneumophila subsp. fraseri strain Detroit-1]
GTGAGAAAAATTATCGCGATTTTGATATTAATTAGTGCCAGTTTGAGTATGGCATATTCTAAAGATAACTCTCTCATTTC
AGTAAAAGTATTACCATTGCCTGAAAACAGAGTACGCATTGACTTTCAATTTTCCAAACCCTTAAAGCAACTGCCTGCAA
GCTTTATTACTCAAAAGCCTCCACGTTTGGTATTGGATTTTATTAGTACCGATATGCAAATTCCCGCCGATCAGAAAACT
AAAACCATTCAATTAGGCTCATTACATAGTTATAACATTGTTGCAGTCAGGGATAGGATAAGGGCCATATTGGAATTGGA
TCGCTCAGTGTCCTATTCTGGCAGTACGGCAGGTTCTGTTTATAGCCTTGTCCTGAATGGTAAAAGCAATGATTTGTTTG
AGAATAGCAAAGAGGTATTCATAACTAATCAGGTTGTGAATGCGAAGCATGAAATTAAAAGAATTGATTTTCGTGGTATA
GAAAAGCAAGGTGGTAGAGTCTTAATTGATGTTTCTGATACTGGTATTCCAATTGATGTGACTCAAGTTGGAAAAGAAAT
AGTCGTTAATTTCCTGAATACCAGGATTCCTTTGAATTTAATGAAACGGTATGACGTTTCTGATTTTCACAGTCCTGTCC
AGATTATCACGATGCAACAGGAAGGCAAAAAAGTTCGTATGACCATCCTCAGTAAAGGAGATTACGGACATTTTGTTTAT
CAGGTCAATAAACAATTTATGGTTGATGTGTTTCCTTTAACTGCGGAAGAAATTCGCCAAGCCAAATTGAAAAAACAGGT
ATTCACTGGCAAACGGATTTCTTTGAATTTCCAGAATATTAGTATTCGAGCCGTGCTTCAACTGCTCGCAGATTTCACTG
GAATCAACATGGTTGTCAGCGACAAAGTGCAAGGAGATATTACGCTTAGATTAAATGATATCCCTTGGGACCAGGCCCTG
GATATTATTTTAACAACACAAGGGTTAGACAAGCGTAGAAAAGGCAATGTCATGTTGATAGATACCAAGGCTTCCATGGA
TAAAATGGAGGAAGATCAACTCAAAAGTCAACAGACAATTGAAAAGCTCGAGCCTATCCGTTCTGATTTAATTCAGATTA
ATTATGCAAAGGCAGCAGATATTGCTGTTTTGATCAAGGATAAGCAAAATTCCTTATTATCAGACAGAGGAAAGATAAGT
GTCGATGCCAGAACGAATACAATCTGGATTCAGGATACTGGCACCAAAATTGAGGAAGTAAGGGAACTTATAAAGCAATT
GGATATTCCTGTTAAGCAAGTACTTATTGAGGCGAGGATTGTTGAAGTAACTAAAGATTTTAGTCAAGATATTGGGATAC
GCTGGGGTGTATCAAGGCCAACTCACTTAAGCGGTACACTTGAGGGTGCTAACGAGTTAGCCAGAGGTACAGCGCCAGCC
AATGTTGTCCCTTTGGAAAGACGGTTGAACCTTGATTTGGCAGCTGCCCCTTTAACTGGAGCTACACCGGCCTCGGTAGG
TATTGCGCTAGCCAAGCTTAATGATAACATACTGCTTGATCTGGAATTGTCTGCTTTGGAAAGTGAGGGGCGTGCTGAAT
TAATATCCAGCCCGCGCCTGATTACTACGAATCAACAAGCAGCAGTCATCGAATCTGGTGAAGAGATTCCCTACCAGGAA
GCGACATCAAGTGGAGCGACTGCTGTAGCATTTAAAAAAGCGGTCTTGAGTTTGAAAGTTACCCCGCAAATTACACCTGA
TGGTAAAATTTTGATGGATTTACAGATAAATCAGGATACTCCTTCTCCACAAACTTTCCTTGGTGTTCCAGCAATAATAA
CAAAGGAGATTCAGACGAATGTTCTGGTTAATAATGGACAAACCATAGTACTTGGGGGTATTTATAAGCAAGATAAGAAC
AAGGTAATCAATAGAATTCCTTTCTTTGGTGAGTTGCCTGTTGTCGGTATTTTATTTAGTAATAGACAAATCACATTGAA
AAATGAAGAATTACTTATCTTTATTACACCAAGAATAATAACGAATGCCTTATCGATTACAACGATAGAGGGACGCGAGA
AAGATGTTTATAAATAA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(39-113)

Bacterial type II and III secretion system protein (Secretin)

(525-680)

Bacterial type II/III secretion system short domain (Secretin_N)

(370-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Legionella pneumophila strain Lp02

99.14

100

0.991
  pilQ Legionella pneumophila strain ERS1305867

98.997

100

0.99
  comQ Acinetobacter baylyi ADP1

38.574

98.424

0.38
  pilQ Pseudomonas aeruginosa PAK

38.102

98.138

0.374
  pilQ Acinetobacter baumannii strain A118

34.959

100

0.37
  pilQ Acinetobacter baumannii D1279779

34.959

100

0.37

Multiple sequence alignment