Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   A4W87_RS03150 Genome accession   NZ_CP017273
Coordinates   624046..624936 (+) Length   296 a.a.
NCBI ID   WP_076632060.1    Uniprot ID   A0AAX0VC29
Organism   Latilactobacillus sakei strain TMW 1.1396     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 619046..629936
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4W87_RS03120 (A4W87_03085) - 619466..620575 (+) 1110 WP_251932814.1 hypothetical protein -
  A4W87_RS03125 (A4W87_03090) - 620535..621935 (+) 1401 WP_076632055.1 hypothetical protein -
  A4W87_RS03135 (A4W87_03100) - 622122..622511 (-) 390 WP_076632056.1 hypothetical protein -
  A4W87_RS03140 (A4W87_03105) - 622608..623108 (+) 501 WP_076632058.1 VanZ family protein -
  A4W87_RS03145 (A4W87_03110) - 623199..623930 (+) 732 WP_076632059.1 YebC/PmpR family DNA-binding transcriptional regulator -
  A4W87_RS03150 (A4W87_03115) comGA 624046..624936 (+) 891 WP_076632060.1 competence type IV pilus ATPase ComGA Machinery gene
  A4W87_RS03155 (A4W87_03120) comGB 624929..625936 (+) 1008 WP_076632061.1 type II secretion system F family protein Machinery gene
  A4W87_RS03160 (A4W87_03125) comGC 625933..626232 (+) 300 WP_076632062.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  A4W87_RS03165 (A4W87_03130) comGD 626204..626656 (+) 453 WP_076632063.1 competence type IV pilus minor pilin ComGD Machinery gene
  A4W87_RS03170 (A4W87_03135) comGE 626643..626948 (+) 306 WP_025016385.1 hypothetical protein Machinery gene
  A4W87_RS03175 (A4W87_03140) comGF 626923..627435 (+) 513 WP_041820873.1 competence type IV pilus minor pilin ComGF Machinery gene
  A4W87_RS03180 (A4W87_03145) - 627503..627736 (+) 234 WP_157779300.1 hypothetical protein -
  A4W87_RS03185 (A4W87_03150) - 627860..628870 (+) 1011 WP_011374994.1 class I SAM-dependent methyltransferase -

Sequence

Protein


Download         Length: 296 a.a.        Molecular weight: 32921.78 Da        Isoelectric Point: 6.7477

>NTDB_id=197214 A4W87_RS03150 WP_076632060.1 624046..624936(+) (comGA) [Latilactobacillus sakei strain TMW 1.1396]
MSTEEKLTTLLKHCAKQRVQDLYFTPTIGGWRLTERRQSDLVTNQELDKPTGTRYLNRLKYMAGMNISETRRAQTGRSEL
QLADQTIYLRLATVGDFLNRESLVIRFIYPIGAIYHCDDQQILAQLTQMSRQAGLILFAGPTGSGKTTSLYHLAQQSMSA
KMVVAIEDPIEIVAPEFLQLQVNDNAGLSYAELLKISLRLRPDTLIIGEIRDLETAQYAVSAALSGHLVLSTIHARSTQG
VVARLLDLGISELQLRACLTGIAYQSLVTKDDVVQAHYELLTGPDYFSKKEGGCDV

Nucleotide


Download         Length: 891 bp        

>NTDB_id=197214 A4W87_RS03150 WP_076632060.1 624046..624936(+) (comGA) [Latilactobacillus sakei strain TMW 1.1396]
ATGTCAACTGAAGAAAAGCTAACAACTTTATTAAAGCATTGTGCTAAACAACGTGTTCAAGATCTTTATTTTACACCGAC
AATAGGTGGCTGGCGCTTGACGGAACGTCGTCAGAGCGATTTGGTCACCAATCAAGAATTGGATAAACCGACTGGAACGC
GTTATTTAAATCGACTGAAGTATATGGCGGGGATGAATATCAGCGAGACTCGGCGTGCGCAAACCGGCCGCTCGGAATTA
CAGCTAGCCGATCAAACAATTTATTTACGCCTAGCGACGGTCGGGGATTTTTTAAACCGCGAAAGCTTGGTCATTCGGTT
TATCTATCCAATTGGTGCCATCTATCATTGTGATGATCAACAGATCTTGGCGCAATTAACGCAGATGAGTCGACAGGCGG
GCTTAATTCTTTTCGCTGGGCCGACTGGTTCTGGTAAAACGACTAGTCTCTATCATCTGGCCCAACAATCAATGAGTGCC
AAGATGGTGGTTGCTATTGAAGATCCGATTGAAATTGTTGCACCTGAGTTTTTGCAACTGCAGGTTAATGATAATGCGGG
ATTGAGCTACGCCGAGCTATTAAAAATTAGTTTACGACTGCGACCGGATACGCTGATTATCGGTGAAATTCGCGATTTAG
AGACAGCTCAATATGCAGTTTCTGCGGCCTTAAGTGGCCACCTGGTATTGAGTACTATTCACGCTCGGAGTACACAGGGC
GTGGTCGCGCGCTTATTAGATCTTGGTATTTCTGAGCTGCAATTGCGGGCCTGTCTAACGGGAATTGCCTACCAATCGTT
GGTGACAAAAGACGACGTTGTGCAAGCCCATTATGAATTATTAACTGGTCCAGATTATTTCTCAAAAAAAGAAGGTGGTT
GCGATGTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Latilactobacillus sakei subsp. sakei 23K

98.649

100

0.986
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

43.019

89.527

0.385
  comGA/cglA/cilD Streptococcus pneumoniae D39

42.642

89.527

0.382
  comGA/cglA/cilD Streptococcus pneumoniae R6

42.642

89.527

0.382
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

42.642

89.527

0.382
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

42.642

89.527

0.382
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

41.241

92.568

0.382
  comGA Lactococcus lactis subsp. cremoris KW2

37.919

100

0.382
  comYA Streptococcus gordonii str. Challis substr. CH1

40.959

91.554

0.375
  comYA Streptococcus mutans UA159

39.777

90.878

0.361
  comYA Streptococcus mutans UA140

38.909

92.905

0.361

Multiple sequence alignment