Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   BHU02_RS05810 Genome accession   NZ_CP017271
Coordinates   1143671..1144378 (-) Length   235 a.a.
NCBI ID   WP_226474265.1    Uniprot ID   -
Organism   Latilactobacillus sakei subsp. sakei strain TMW 1.1189     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1138671..1149378
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BHU02_RS05785 (BHU02_05670) rpsO 1138966..1139235 (-) 270 WP_011374766.1 30S ribosomal protein S15 -
  BHU02_RS05790 (BHU02_05675) rpsT 1139514..1139768 (+) 255 WP_011374767.1 30S ribosomal protein S20 -
  BHU02_RS05795 (BHU02_05680) holA 1139836..1140864 (-) 1029 WP_025015735.1 DNA polymerase III subunit delta -
  BHU02_RS05800 (BHU02_05685) comEC 1140861..1142927 (-) 2067 WP_231953244.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  BHU02_RS05805 (BHU02_05690) - 1143125..1143613 (-) 489 WP_011374770.1 ComE operon protein 2 -
  BHU02_RS05810 (BHU02_05695) comEA 1143671..1144378 (-) 708 WP_226474265.1 helix-hairpin-helix domain-containing protein Machinery gene
  BHU02_RS05815 (BHU02_05700) - 1144452..1145498 (-) 1047 WP_035145546.1 SepM family pheromone-processing serine protease -
  BHU02_RS05820 (BHU02_05705) coaD 1145500..1145997 (-) 498 WP_025015732.1 pantetheine-phosphate adenylyltransferase -
  BHU02_RS05825 (BHU02_05710) rsmD 1146010..1146564 (-) 555 WP_016265217.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  BHU02_RS05830 (BHU02_05715) - 1146569..1146859 (-) 291 WP_016265218.1 YlbG family protein -
  BHU02_RS05835 (BHU02_05720) - 1146883..1147971 (-) 1089 WP_025015731.1 CAP-associated domain-containing protein -
  BHU02_RS05840 (BHU02_05725) - 1147982..1149169 (-) 1188 WP_025015730.1 FtsW/RodA/SpoVE family cell cycle protein -

Sequence

Protein


Download         Length: 235 a.a.        Molecular weight: 26122.75 Da        Isoelectric Point: 9.7683

>NTDB_id=197155 BHU02_RS05810 WP_226474265.1 1143671..1144378(-) (comEA) [Latilactobacillus sakei subsp. sakei strain TMW 1.1189]
MMWQDYWQRYRRFWWVPCLVLLGIITGGFYWHQTQQKQKVTHNEQLLATSASKIKPATKKTHQAATKKKGGFVDIKGAVA
YPGIYPITVSETRLFEVLKNAGGPTDAADTKRLNLAQKLTDQLIIYIPKKGEVVTDQELIQAPQQIATTSERTPEQASTG
TSANASSASDTAKIDLNQADSTQLQTLNGIGPKKAEQIIAYRDEQGKFKQIEDLQKVGGIGPKTFEQLQSQICVN

Nucleotide


Download         Length: 708 bp        

>NTDB_id=197155 BHU02_RS05810 WP_226474265.1 1143671..1144378(-) (comEA) [Latilactobacillus sakei subsp. sakei strain TMW 1.1189]
ATGATGTGGCAAGATTATTGGCAGCGATATCGGCGTTTTTGGTGGGTGCCTTGCTTAGTGCTATTGGGGATCATCACGGG
TGGCTTTTATTGGCATCAAACGCAACAAAAACAAAAAGTAACGCATAATGAGCAACTGCTAGCAACCAGTGCGTCTAAAA
TAAAACCTGCTACTAAGAAAACACACCAAGCGGCAACCAAGAAAAAAGGCGGTTTTGTCGATATAAAAGGTGCTGTGGCT
TATCCGGGAATTTATCCGATCACAGTCTCTGAAACGCGCTTATTTGAGGTGCTCAAAAATGCAGGTGGTCCGACTGATGC
GGCTGATACCAAGCGGCTTAATTTAGCCCAAAAATTAACCGATCAACTGATTATTTATATTCCGAAAAAAGGAGAGGTGG
TTACTGATCAAGAACTCATTCAAGCGCCACAACAAATTGCAACGACCAGTGAGCGTACGCCAGAACAAGCATCAACTGGG
ACAAGTGCGAATGCATCGAGCGCGAGTGACACTGCTAAAATCGATTTGAATCAGGCTGATAGTACGCAGCTTCAGACCCT
CAATGGCATTGGCCCCAAGAAAGCTGAACAAATAATTGCTTATCGTGATGAACAAGGAAAATTTAAACAGATTGAAGATC
TACAAAAAGTCGGCGGGATTGGTCCGAAGACGTTTGAACAATTGCAATCACAAATTTGTGTGAATTAG

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(73-124)

Helix-hairpin-helix motif (HHH_3)

(172-233)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Latilactobacillus sakei subsp. sakei 23K

97.021

100

0.97

Multiple sequence alignment