Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   BHS01_RS01045 Genome accession   NZ_CP017195
Coordinates   206568..207509 (+) Length   313 a.a.
NCBI ID   WP_191246366.1    Uniprot ID   -
Organism   Lactococcus paracarnosus strain TMW 2.1615     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 201568..212509
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BHS01_RS01040 (BHS01_01030) - 202049..206443 (+) 4395 WP_109833904.1 PolC-type DNA polymerase III -
  BHS01_RS01045 (BHS01_01035) comGA/cglA/cilD 206568..207509 (+) 942 WP_191246366.1 competence type IV pilus ATPase ComGA Machinery gene
  BHS01_RS01050 (BHS01_01040) comGB 207451..208476 (+) 1026 WP_162542379.1 competence type IV pilus assembly protein ComGB -
  BHS01_RS01055 (BHS01_01045) comGC/cglC 208479..208826 (+) 348 WP_109833907.1 competence type IV pilus major pilin ComGC Machinery gene
  BHS01_RS01060 (BHS01_01050) comGD 208798..209226 (+) 429 WP_162542380.1 competence type IV pilus minor pilin ComGD -
  BHS01_RS01065 (BHS01_01055) comGE 209198..209503 (+) 306 WP_162542381.1 competence type IV pilus minor pilin ComGE -
  BHS01_RS01070 (BHS01_01060) comGF 209478..209921 (+) 444 WP_223271025.1 competence type IV pilus minor pilin ComGF -
  BHS01_RS01075 (BHS01_01065) comGG 209933..210217 (+) 285 WP_109833910.1 competence type IV pilus minor pilin ComGG -
  BHS01_RS01080 (BHS01_01070) - 210427..210846 (+) 420 WP_109833911.1 GNAT family N-acetyltransferase -
  BHS01_RS01085 (BHS01_01075) ispE 210942..211793 (+) 852 WP_109833912.1 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase -
  BHS01_RS01090 (BHS01_01080) - 211922..212371 (+) 450 WP_109833913.1 zinc-dependent MarR family transcriptional regulator -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 34602.84 Da        Isoelectric Point: 9.8723

>NTDB_id=196556 BHS01_RS01045 WP_191246366.1 206568..207509(+) (comGA/cglA/cilD) [Lactococcus paracarnosus strain TMW 2.1615]
MVQKLAKVLLQAAVSFGASDVYVLPVETTFAVSFRRSESRKHYITCSAAEGQSLISHFKFTAGMNVGEKRRPQLGACTYE
IGERSLRLRLSTAGDFENRESLVIRLLPDQAHPLLFWDKATLLSLKTAVVSRGLYLFSGPVGSGKTTLMHHVAKENFTDK
QVITIEDPVELVRPELLQFQINEAIGNTYDSLIKLSLRHMPDLVIVGEIRDSTTARAVIRASLTGYTVFSTIHAKSIRGV
YARLLALGVTKDELENALSGIIYQRLIAGKGVLTYADKNFSNHSHDGWNRQIEALVRSGSIRASQATVEKIIS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=196556 BHS01_RS01045 WP_191246366.1 206568..207509(+) (comGA/cglA/cilD) [Lactococcus paracarnosus strain TMW 2.1615]
ATGGTTCAGAAATTAGCAAAAGTACTCTTACAAGCGGCAGTTAGCTTTGGTGCAAGCGATGTCTATGTTTTACCAGTTGA
GACGACGTTTGCAGTTAGCTTTAGGCGTTCGGAGAGTCGTAAGCACTATATCACTTGTTCAGCTGCTGAGGGGCAAAGTT
TAATCTCACATTTCAAATTTACAGCTGGTATGAACGTTGGCGAAAAGCGACGCCCGCAGTTAGGTGCCTGTACCTATGAG
ATTGGTGAGCGCAGTCTGAGGCTAAGACTATCTACAGCAGGGGATTTTGAAAATCGAGAAAGTCTGGTCATTCGATTATT
GCCAGATCAAGCGCATCCCCTCTTGTTTTGGGATAAAGCGACGCTTCTATCATTGAAAACGGCGGTAGTCAGTCGAGGGC
TTTATCTATTTTCAGGACCGGTGGGTTCCGGAAAGACGACACTGATGCACCATGTTGCAAAAGAGAACTTTACAGATAAA
CAGGTCATTACGATAGAAGACCCAGTAGAACTTGTCAGGCCAGAGCTTTTGCAATTCCAAATCAATGAAGCGATTGGCAA
CACCTATGATAGCTTAATTAAATTATCTCTTAGACACATGCCGGATCTTGTCATTGTAGGAGAAATTCGAGATAGTACGA
CAGCTCGTGCGGTCATCCGGGCTAGTTTGACGGGCTACACGGTATTTTCGACGATTCATGCCAAATCTATTCGTGGTGTA
TATGCTAGGTTACTCGCCTTAGGCGTGACCAAAGATGAGCTAGAAAATGCCTTGTCTGGTATTATTTATCAGCGACTCAT
AGCAGGAAAAGGGGTGCTAACCTATGCAGATAAAAACTTCTCTAATCACAGCCATGACGGATGGAATAGGCAGATTGAGG
CACTTGTTAGATCAGGATCTATTCGTGCTAGTCAGGCGACGGTCGAAAAAATTATCAGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

55.128

99.681

0.55
  comGA Lactococcus lactis subsp. cremoris KW2

54.808

99.681

0.546
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

54.984

99.361

0.546
  comYA Streptococcus mutans UA159

54.167

99.681

0.54
  comYA Streptococcus mutans UA140

54.167

99.681

0.54
  comYA Streptococcus gordonii str. Challis substr. CH1

54.341

99.361

0.54
  comGA/cglA/cilD Streptococcus pneumoniae D39

53.055

99.361

0.527
  comGA/cglA/cilD Streptococcus pneumoniae R6

53.055

99.361

0.527
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

53.055

99.361

0.527
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

53.055

99.361

0.527

Multiple sequence alignment