Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA2   Type   Machinery gene
Locus tag   clem_RS10210 Genome accession   NZ_CP016397
Coordinates   2332967..2333374 (-) Length   135 a.a.
NCBI ID   WP_094091459.1    Uniprot ID   A0A222P436
Organism   Legionella clemsonensis strain CDC-D5610     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 2327967..2338374
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  clem_RS10200 (clem_10360) - 2328685..2329923 (+) 1239 WP_094091457.1 6-phosphofructokinase -
  clem_RS10205 (clem_10365) - 2330106..2332934 (+) 2829 WP_094091458.1 ankyrin repeat domain-containing protein -
  clem_RS10210 (clem_10370) pilA2 2332967..2333374 (-) 408 WP_094091459.1 pilin Machinery gene
  clem_RS10215 (clem_10375) - 2333792..2334109 (+) 318 WP_094091460.1 BolA family transcriptional regulator -
  clem_RS10220 (clem_10380) - 2334102..2334563 (+) 462 WP_094091461.1 secondary thiamine-phosphate synthase enzyme YjbQ -
  clem_RS10225 (clem_10385) - 2334537..2334950 (-) 414 WP_094091462.1 hypothetical protein -
  clem_RS10230 (clem_10390) - 2335403..2336830 (+) 1428 WP_094091463.1 APC family permease -
  clem_RS10235 (clem_10395) pheA 2336827..2337906 (+) 1080 WP_094091464.1 prephenate dehydratase -

Sequence


Protein


Download         Length: 135 a.a.        Molecular weight: 14218.58 Da        Isoelectric Point: 7.8133

>NTDB_id=187975 clem_RS10210 WP_094091459.1 2332967..2333374(-) (pilA2) [Legionella clemsonensis strain CDC-D5610]
MKEKGFTLIELMIVVAILGILISLAVPAYRQHIVRAKVIEGLSLASTAKIAVTEAAVANQALPASQETTGYTSPAPTANV
KSITIGDKGVITITYTPEAGDGTLLLTPTLRTNGDIIWTCAGGTLEEKYRPYGCK

Nucleotide


Download         Length: 408 bp        

>NTDB_id=187975 clem_RS10210 WP_094091459.1 2332967..2333374(-) (pilA2) [Legionella clemsonensis strain CDC-D5610]
ATGAAAGAAAAAGGGTTTACATTAATTGAATTAATGATCGTGGTAGCTATTCTAGGTATTCTAATTTCTCTTGCCGTACC
TGCTTACAGACAGCACATCGTACGAGCGAAAGTGATCGAGGGTTTGAGTTTAGCCTCTACTGCCAAAATTGCAGTCACTG
AAGCTGCGGTGGCTAATCAGGCATTACCTGCCTCACAGGAAACCACAGGCTACACTAGCCCGGCTCCTACTGCTAATGTG
AAATCAATCACTATTGGTGATAAAGGGGTAATTACTATCACCTACACACCTGAAGCAGGTGATGGAACGCTTCTGTTAAC
TCCAACATTGAGAACTAATGGCGATATAATCTGGACTTGTGCAGGGGGTACTTTGGAAGAAAAATACCGACCTTATGGCT
GCAAGTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A222P436

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

63.704

100

0.637

  pilA2 Legionella pneumophila strain ERS1305867

63.704

100

0.637

  pilA Ralstonia pseudosolanacearum GMI1000

44.099

100

0.526

  comP Acinetobacter baylyi ADP1

40.69

100

0.437

  pilA Acinetobacter baumannii strain A118

37.956

100

0.385

  pilE Neisseria gonorrhoeae strain FA1090

32.692

100

0.378

  pilE Neisseria gonorrhoeae MS11

39.683

93.333

0.37

  pilA/pilAI Pseudomonas stutzeri DSM 10701

36.496

100

0.37

  pilA/pilA1 Eikenella corrodens VA1

33.108

100

0.363


Multiple sequence alignment