Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   BBB50_RS01650 Genome accession   NZ_CP016324
Coordinates   313688..315403 (+) Length   571 a.a.
NCBI ID   WP_000788426.1    Uniprot ID   A0A0K9UP29
Organism   Vibrio cholerae 2740-80     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 277611..315403 313688..315403 within 0


Gene organization within MGE regions


Location: 277611..315403
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BBB50_RS01355 (BBB50_01355) - 277611..278147 (-) 537 WP_001065600.1 hypothetical protein -
  BBB50_RS01360 (BBB50_01360) - 278147..278998 (-) 852 WP_001895339.1 hypothetical protein -
  BBB50_RS01365 (BBB50_01365) - 278998..279585 (-) 588 WP_000510163.1 YmfQ family protein -
  BBB50_RS01370 (BBB50_01370) - 279570..280637 (-) 1068 WP_001147110.1 baseplate J/gp47 family protein -
  BBB50_RS01375 (BBB50_01375) - 280627..281076 (-) 450 WP_000095018.1 phage GP46 family protein -
  BBB50_RS01380 (BBB50_01380) - 281079..281696 (-) 618 WP_170826454.1 phage baseplate assembly protein V -
  BBB50_RS01385 (BBB50_01385) - 281690..282769 (-) 1080 WP_001286435.1 phage baseplate assembly protein -
  BBB50_RS01390 (BBB50_01390) - 282762..284081 (-) 1320 WP_000863380.1 DNA circularization protein -
  BBB50_RS19195 - 284093..285796 (-) 1704 WP_001284562.1 tape measure protein -
  BBB50_RS01405 (BBB50_01405) - 285922..286281 (-) 360 WP_000996598.1 phage tail assembly protein -
  BBB50_RS01410 (BBB50_01410) - 286281..286634 (-) 354 WP_001895328.1 phage tail tube protein -
  BBB50_RS01415 (BBB50_01415) - 286649..288133 (-) 1485 WP_001128141.1 phage tail sheath subtilisin-like domain-containing protein -
  BBB50_RS01420 (BBB50_01420) - 288136..288336 (-) 201 WP_000437435.1 DUF2635 domain-containing protein -
  BBB50_RS01425 (BBB50_01425) - 288350..288949 (-) 600 WP_000100991.1 hypothetical protein -
  BBB50_RS01430 (BBB50_01430) - 288946..289488 (-) 543 WP_000513061.1 phage virion morphogenesis protein -
  BBB50_RS01435 (BBB50_01435) - 289488..289922 (-) 435 WP_001029276.1 gp436 family protein -
  BBB50_RS01440 (BBB50_01440) - 289928..290503 (-) 576 WP_000004756.1 hypothetical protein -
  BBB50_RS01445 (BBB50_01445) - 290592..291491 (-) 900 WP_001218331.1 Mu-like prophage major head subunit gpT family protein -
  BBB50_RS01450 (BBB50_01450) - 291494..292450 (-) 957 WP_001262460.1 phage protease -
  BBB50_RS01455 (BBB50_01455) - 292783..293067 (-) 285 WP_001249579.1 hypothetical protein -
  BBB50_RS01460 (BBB50_01460) - 293067..293702 (-) 636 WP_000638762.1 hypothetical protein -
  BBB50_RS01465 (BBB50_01465) - 293699..294211 (-) 513 WP_000638754.1 tail needle knob protein -
  BBB50_RS01470 (BBB50_01470) - 294208..294456 (-) 249 WP_000246042.1 hypothetical protein -
  BBB50_RS01475 (BBB50_01475) - 294468..295250 (-) 783 WP_412034587.1 phage minor head protein -
  BBB50_RS01480 (BBB50_01480) - 295234..296793 (-) 1560 WP_000027033.1 DUF935 domain-containing protein -
  BBB50_RS01485 (BBB50_01485) - 296790..298355 (-) 1566 WP_001105344.1 terminase family protein -
  BBB50_RS01490 (BBB50_01490) - 298356..298922 (-) 567 WP_001195537.1 DUF3486 family protein -
  BBB50_RS01495 (BBB50_01495) - 298934..299224 (-) 291 WP_000008606.1 hypothetical protein -
  BBB50_RS01500 (BBB50_01500) - 299233..299532 (-) 300 WP_000362983.1 DUF2730 family protein -
  BBB50_RS01505 (BBB50_01505) - 299532..299762 (-) 231 WP_001281317.1 TraR/DksA C4-type zinc finger protein -
  BBB50_RS01510 (BBB50_01510) - 299759..300358 (-) 600 WP_001120998.1 hypothetical protein -
  BBB50_RS21080 (BBB50_01515) - 300334..300621 (-) 288 WP_000828640.1 hypothetical protein -
  BBB50_RS01520 (BBB50_01520) - 300621..301178 (-) 558 WP_000493682.1 glycoside hydrolase family 108 protein -
  BBB50_RS01525 (BBB50_01525) - 301263..301679 (-) 417 WP_001029055.1 Mor transcription activator family protein -
  BBB50_RS01530 (BBB50_01530) - 301758..302204 (-) 447 WP_000837433.1 hypothetical protein -
  BBB50_RS01535 (BBB50_01535) - 302194..302739 (-) 546 WP_000040562.1 gp16 family protein -
  BBB50_RS01540 (BBB50_01540) - 302752..303021 (-) 270 WP_230078620.1 hypothetical protein -
  BBB50_RS01545 (BBB50_01545) - 303033..303266 (-) 234 WP_000466681.1 hypothetical protein -
  BBB50_RS01555 (BBB50_01555) - 303346..303876 (-) 531 WP_000762331.1 hypothetical protein -
  BBB50_RS01560 (BBB50_01560) - 303873..304142 (-) 270 WP_000856884.1 DUF3850 domain-containing protein -
  BBB50_RS20590 (BBB50_01565) - 304145..304495 (-) 351 WP_001066644.1 hypothetical protein -
  BBB50_RS01570 (BBB50_01570) - 304492..304689 (-) 198 WP_000413231.1 hypothetical protein -
  BBB50_RS01575 (BBB50_01575) - 304673..304915 (-) 243 WP_000551393.1 hypothetical protein -
  BBB50_RS01580 (BBB50_01580) - 304908..305180 (-) 273 WP_000512064.1 hypothetical protein -
  BBB50_RS01585 (BBB50_01585) - 305190..305804 (-) 615 WP_001192102.1 DUF3164 family protein -
  BBB50_RS01595 (BBB50_01595) - 305954..306328 (-) 375 WP_001032752.1 hypothetical protein -
  BBB50_RS01600 (BBB50_01600) - 306338..306907 (-) 570 WP_001216028.1 hypothetical protein -
  BBB50_RS01605 (BBB50_01605) - 306917..307141 (-) 225 WP_000157299.1 hypothetical protein -
  BBB50_RS01610 (BBB50_01610) - 307149..308093 (-) 945 WP_000800334.1 AAA family ATPase -
  BBB50_RS01615 (BBB50_01615) - 308120..310123 (-) 2004 WP_001895336.1 transposase domain-containing protein -
  BBB50_RS01620 (BBB50_01620) - 310135..310359 (-) 225 WP_000373567.1 helix-turn-helix domain-containing protein -
  BBB50_RS01625 (BBB50_01625) - 310512..311249 (+) 738 WP_000005494.1 LexA family transcriptional regulator -
  BBB50_RS01630 (BBB50_01630) pilM 311518..311997 (+) 480 Protein_293 type IV pilus assembly protein PilM -
  BBB50_RS01635 (BBB50_01635) pilN 311987..312571 (+) 585 WP_000902745.1 PilN domain-containing protein Machinery gene
  BBB50_RS01640 (BBB50_01640) pilO 312564..313154 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  BBB50_RS01645 (BBB50_01645) pilP 313144..313662 (+) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  BBB50_RS01650 (BBB50_01650) pilQ 313688..315403 (+) 1716 WP_000788426.1 type IV pilus secretin PilQ Machinery gene

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62340.49 Da        Isoelectric Point: 4.6119

>NTDB_id=186916 BBB50_RS01650 WP_000788426.1 313688..315403(+) (pilQ) [Vibrio cholerae 2740-80]
MKNGLKTYVAQTWLTLWVGLALCASSMVFSAESATANQLENIDFRVNKEKAAVLIVELASPSAVVDVQKVQEGLSIELLK
TDVADDKLYLLDVKDFSTPVESVEVFRKEPSTQLVVTVDGEFQHDYTLKGKYLEVVISKLKADEKPKPKSVLEKEGKLIS
INFQDIPVRNVLQLIADYNGFNLVVSDSVVGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILIAPKEELDLREKQAL
EKARLAEELGDLKSEIIKINFAKASDIAAMIGGEGNVNMLSERGSISIDERTNSLLIRELPDNIAVIREIIESLDIPVKQ
VQIEARIVTVKEGNLEELGVRWGVMSTNGSHSVGGSIESNLWQKGLLADDEFPVDEFLNVNLASTSANASSIAFQVAKLG
SGTLLDLELSALQNESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGASTVAFKKAVLSLKVTPQITPDNRLVLDL
SVTQDRRGETVKTGTGEAVSIDTQRIGTQVLVNNGETVVLGGIFQHSINNSVDKVPLLGDLPVLGALFRRTYEQMGKSEL
LIFVTPKVVIQ

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=186916 BBB50_RS01650 WP_000788426.1 313688..315403(+) (pilQ) [Vibrio cholerae 2740-80]
ATGAAAAATGGATTGAAAACTTATGTCGCACAGACTTGGCTGACCTTGTGGGTTGGTTTGGCACTTTGTGCTTCTTCCAT
GGTGTTTAGTGCTGAATCCGCTACCGCCAACCAGTTGGAAAACATCGACTTTCGGGTCAACAAAGAGAAGGCGGCGGTCT
TAATTGTCGAATTGGCTTCGCCATCGGCGGTCGTGGATGTGCAAAAAGTCCAAGAAGGGTTGAGCATTGAACTCCTGAAA
ACCGATGTGGCGGATGACAAGCTGTATCTGCTTGATGTGAAAGATTTTTCGACCCCTGTTGAGAGCGTGGAAGTATTCCG
TAAAGAGCCAAGCACACAACTGGTTGTGACTGTCGATGGGGAGTTTCAGCACGATTATACCTTAAAAGGAAAATACCTTG
AGGTCGTGATCAGTAAACTCAAAGCCGATGAAAAGCCTAAGCCCAAGAGTGTCTTGGAAAAAGAAGGCAAGCTGATTTCA
ATTAACTTCCAAGATATTCCGGTGCGTAACGTACTGCAGCTGATTGCCGATTATAACGGTTTCAACTTAGTGGTTTCCGA
TTCGGTGGTGGGTAACCTGACTTTACGCTTGGATGGCGTGCCTTGGCAGCAAGTGTTGGACATTATTCTGCAAGTCAAAG
GGCTCGATAAACGGGTCGATGGTAATGTGATTTTGATTGCGCCGAAAGAGGAACTCGACTTGCGCGAGAAACAAGCGCTC
GAAAAAGCCCGTTTAGCAGAAGAGTTGGGCGATTTAAAATCAGAAATCATCAAGATTAACTTTGCCAAAGCGTCTGACAT
TGCGGCGATGATCGGCGGTGAAGGCAACGTTAACATGTTGTCTGAACGAGGTTCGATCAGCATCGACGAACGAACTAACT
CGCTATTGATCCGAGAGCTGCCCGATAATATTGCTGTGATCCGCGAGATCATTGAATCGCTGGATATTCCGGTCAAACAA
GTTCAAATCGAAGCGCGGATTGTCACCGTCAAAGAAGGTAACTTGGAGGAGCTGGGGGTACGCTGGGGCGTGATGTCGAC
CAATGGTAGCCACTCGGTCGGAGGAAGCATAGAGAGTAACTTATGGCAAAAAGGGTTATTGGCGGATGATGAATTTCCGG
TGGATGAATTCTTAAATGTGAATTTGGCTTCCACCTCTGCCAATGCGTCTTCGATTGCTTTCCAAGTCGCTAAATTGGGC
TCAGGTACGTTGTTGGATTTGGAGCTTTCAGCATTACAAAATGAATCGAAGGCCGAAATCATCTCTAGCCCTCGTTTGAT
TACCACTAATAAACAGCCTGCCTATATTGAGCAAGGTACTGAGATCCCTTATCTGGAATCGTCTTCCAGTGGTGCCTCTA
CGGTCGCGTTTAAAAAAGCGGTATTAAGCTTGAAGGTGACACCACAAATCACCCCAGATAACCGCTTGGTGCTCGACTTG
AGTGTGACCCAAGACCGACGCGGTGAAACGGTGAAAACCGGTACGGGTGAAGCGGTATCGATCGATACTCAGCGTATTGG
CACTCAAGTGTTGGTGAATAACGGAGAAACTGTGGTGTTGGGTGGCATTTTCCAACACAGCATCAATAATTCGGTAGATA
AGGTGCCACTGCTGGGGGACTTGCCTGTTTTGGGGGCGTTATTCAGACGAACCTATGAGCAAATGGGCAAAAGTGAACTG
CTGATTTTTGTCACGCCGAAAGTGGTCATTCAATAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(411-569)

Bacterial type II/III secretion system short domain (Secretin_N)

(255-320)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K9UP29

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

100

100

1
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

100

100

1
  pilQ Vibrio campbellii strain DS40M4

78.545

96.322

0.757
  pilQ Legionella pneumophila strain ERS1305867

42.778

94.571

0.405
  pilQ Legionella pneumophila strain Lp02

42.778

94.571

0.405
  comQ Acinetobacter baylyi ADP1

39.336

100

0.394
  pilQ Acinetobacter baumannii strain A118

37.819

100

0.389
  pilQ Acinetobacter baumannii D1279779

37.819

100

0.389
  pilQ Pseudomonas aeruginosa PAK

39.344

96.147

0.378

Multiple sequence alignment