Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BA177_RS14080 Genome accession   NZ_CP016268
Coordinates   3113688..3115409 (+) Length   573 a.a.
NCBI ID   WP_068617238.1    Uniprot ID   A0A193LI54
Organism   Woeseia oceani strain XK5     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3108688..3120409
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BA177_RS14060 (BA177_14060) - 3109074..3110744 (+) 1671 WP_068617225.1 PAS domain-containing sensor histidine kinase -
  BA177_RS14065 (BA177_14065) pilR 3110769..3112085 (+) 1317 WP_068617232.1 sigma-54 dependent transcriptional regulator Regulator
  BA177_RS19190 (BA177_14070) - 3112337..3112798 (+) 462 Protein_2752 pilin -
  BA177_RS14075 (BA177_14075) - 3113013..3113516 (+) 504 WP_068617235.1 pilin -
  BA177_RS14080 (BA177_14080) pilB 3113688..3115409 (+) 1722 WP_068617238.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BA177_RS14085 (BA177_14085) pilC 3115444..3116664 (+) 1221 WP_068617240.1 type II secretion system F family protein Machinery gene
  BA177_RS14090 (BA177_14090) pilD 3116754..3117638 (+) 885 WP_068617245.1 A24 family peptidase Machinery gene
  BA177_RS14095 (BA177_14095) coaE 3117654..3118277 (+) 624 WP_068617251.1 dephospho-CoA kinase -
  BA177_RS14100 (BA177_14100) zapD 3118358..3119146 (+) 789 WP_197493069.1 cell division protein ZapD -
  BA177_RS14105 (BA177_14105) mutT 3119168..3119578 (-) 411 WP_082990125.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62609.10 Da        Isoelectric Point: 5.1755

>NTDB_id=186427 BA177_RS14080 WP_068617238.1 3113688..3115409(+) (pilB) [Woeseia oceani strain XK5]
MAVSASQSTLSGLPRRLVQDGIIAEQAILDAAEAAKKAHLPLVAYLVSNELADSRAIAVAASHEFGVPLLDLDAIEVDLE
CLRAVDQKLLNKHRVLPLVKRGQRLFLGISDPTNLQAIDEVKFQTSLRVDPVVVEQAKLEERISKALEAVDTTMSGLDDD
DFDLENLDVSSGEEEQEDGITRDDIEDAPVVRFVNKVLLDAIKRGASDIHFEPYEKIFRVRTRLDGVLSEVAQPPVALAN
KVCARLKVMSRMDIAERRVPQDGRIKMRLSKNRAIDFRVNTCPTLFGEKIVLRILDPSSAKLGIDALGYDETQKALYLKH
LNKPYGMILVTGPTGSGKTVSLYTGLNILNTEDRNISTAEDPAEINLPGINQVNVNPKVGLTFASSLKAFLRQDPDVIMV
GEIRDLETAEIAIKAAQTGHLVLSTLHTNDAPKTLTRLVDMGVKPYAIATSVSLIIAQRLARKLCDNCKEVRDIPHEALL
KEGFAEADIKAGLSVFGPKGCKLCNEGYKGRLGLFQVMEVSETMGRIIMEGGNAMQIADQAAKEGVIDLRTAGLNKVKDG
ITSLEEINRVTIE

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=186427 BA177_RS14080 WP_068617238.1 3113688..3115409(+) (pilB) [Woeseia oceani strain XK5]
ATGGCAGTATCGGCATCTCAATCGACGCTTTCGGGCCTTCCACGGCGCCTGGTCCAGGACGGGATCATTGCTGAACAGGC
CATCCTTGACGCCGCAGAAGCGGCCAAGAAAGCACATCTGCCGTTAGTCGCCTACCTCGTATCCAACGAACTGGCCGATT
CCCGCGCAATCGCGGTCGCGGCCTCGCACGAGTTCGGCGTGCCTTTGCTGGATCTGGATGCGATAGAAGTGGATCTCGAG
TGCCTGCGGGCAGTCGATCAGAAACTCCTGAACAAGCACCGTGTGCTGCCTTTGGTCAAACGCGGCCAGCGGCTATTCCT
GGGTATCTCTGACCCAACCAATTTACAGGCTATCGACGAGGTCAAGTTTCAGACCAGCCTGCGTGTTGATCCGGTGGTTG
TCGAGCAGGCAAAACTGGAAGAACGCATCAGCAAAGCGCTGGAAGCGGTCGACACGACCATGTCCGGCCTCGACGATGAC
GACTTCGACCTGGAGAACCTCGACGTATCAAGCGGCGAAGAAGAGCAGGAAGATGGCATCACCCGTGATGACATCGAAGA
CGCGCCGGTTGTTCGTTTCGTTAACAAGGTTTTGCTCGACGCGATCAAACGCGGCGCATCGGATATCCATTTCGAACCTT
ACGAGAAAATCTTCCGCGTACGCACACGACTTGACGGCGTGCTTTCCGAAGTTGCACAACCGCCGGTCGCGCTTGCCAAC
AAGGTATGTGCGCGACTCAAAGTAATGTCGCGCATGGACATTGCCGAGCGCCGCGTACCACAGGACGGTCGCATCAAAAT
GCGTTTGTCCAAAAACCGCGCTATCGATTTTCGTGTCAATACCTGCCCGACCTTGTTCGGCGAGAAAATCGTATTACGTA
TTCTGGATCCCAGCAGCGCCAAGCTTGGCATTGACGCACTGGGCTATGACGAAACGCAGAAGGCGCTGTACCTAAAGCAC
CTCAACAAGCCGTACGGAATGATTTTGGTTACCGGCCCGACAGGTTCGGGTAAAACCGTTTCGCTGTATACCGGCCTCAA
TATTCTGAACACGGAAGACCGCAATATTTCGACGGCGGAAGATCCGGCGGAAATCAACCTGCCTGGCATCAACCAGGTTA
ACGTCAATCCGAAAGTTGGACTCACGTTTGCCTCATCGCTGAAAGCGTTTCTGCGACAGGATCCCGACGTCATCATGGTC
GGTGAGATTCGTGACCTGGAAACCGCCGAGATCGCGATCAAGGCGGCACAAACCGGTCACCTTGTACTTTCCACACTGCA
TACCAATGACGCGCCGAAAACACTGACGCGACTCGTCGACATGGGCGTCAAGCCTTACGCTATTGCGACCTCAGTAAGCT
TGATCATTGCGCAACGGCTTGCGCGCAAGTTGTGTGACAACTGCAAGGAAGTGCGTGACATTCCGCACGAAGCCTTACTC
AAAGAAGGCTTCGCGGAAGCAGACATCAAAGCAGGGCTTTCAGTATTCGGGCCGAAGGGCTGCAAGTTATGCAATGAAGG
GTACAAAGGCCGCCTCGGCTTGTTCCAGGTCATGGAAGTCTCAGAGACCATGGGACGCATCATCATGGAGGGTGGCAATG
CCATGCAGATAGCCGATCAAGCTGCCAAGGAAGGTGTCATCGATCTTAGAACGGCCGGACTGAACAAAGTCAAGGACGGG
ATCACAAGTCTTGAAGAGATCAACCGGGTTACAATTGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-463)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A193LI54

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.565

100

0.578
  pilB Acinetobacter baylyi ADP1

55.923

100

0.56
  pilB Legionella pneumophila strain ERS1305867

51.826

100

0.52
  pilF Neisseria gonorrhoeae MS11

48.49

98.255

0.476
  pilB Vibrio parahaemolyticus RIMD 2210633

48.142

98.604

0.475
  pilB Vibrio campbellii strain DS40M4

47.972

98.953

0.475
  pilB Vibrio cholerae strain A1552

51.556

89.703

0.462
  pilF Thermus thermophilus HB27

37.197

100

0.375
  pilB/pilB1 Synechocystis sp. PCC 6803

40.426

90.227

0.365

Multiple sequence alignment