Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbB   Type   Machinery gene
Locus tag   LLJM2_RS02190 Genome accession   NZ_CP015900
Coordinates   436623..437012 (+) Length   129 a.a.
NCBI ID   WP_021165898.1    Uniprot ID   A0A166Z4Y5
Organism   Lactococcus cremoris strain JM2     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 436618..448227 436623..437012 within 0


Gene organization within MGE regions


Location: 436618..448227
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLJM2_RS02190 (LLJM2_0423) ssbB 436623..437012 (+) 390 WP_021165898.1 single-stranded DNA-binding protein Machinery gene
  LLJM2_RS02195 (LLJM2_0424) groES 437131..437415 (+) 285 WP_011675464.1 co-chaperone GroES -
  LLJM2_RS02200 (LLJM2_0425) groL 437502..439130 (+) 1629 WP_021213999.1 chaperonin GroEL -
  LLJM2_RS02205 (LLJM2_0426) - 439182..439994 (-) 813 WP_011675466.1 MBL fold metallo-hydrolase -
  LLJM2_RS02210 (LLJM2_0427) - 440184..441611 (-) 1428 WP_011675468.1 cell wall metabolism sensor histidine kinase WalK -
  LLJM2_RS02215 (LLJM2_0428) yycF 441604..442305 (-) 702 WP_003131580.1 response regulator YycF -
  LLJM2_RS02220 (LLJM2_0429) tmk 442483..443118 (+) 636 WP_015082045.1 dTMP kinase -
  LLJM2_RS02225 (LLJM2_0430) - 443251..444111 (+) 861 WP_011675470.1 DNA polymerase III subunit delta' -
  LLJM2_RS02230 (LLJM2_0431) ricT 444161..444943 (+) 783 WP_011834464.1 stage 0 sporulation family protein -
  LLJM2_RS02235 (LLJM2_0432) yabA 444936..445262 (+) 327 WP_011675472.1 DNA replication initiation control protein YabA -
  LLJM2_RS02240 (LLJM2_0433) rsmI 445262..446137 (+) 876 WP_011834466.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  LLJM2_RS02245 (LLJM2_0434) - 446215..446406 (-) 192 WP_011675474.1 hypothetical protein -
  LLJM2_RS02250 (LLJM2_0435) - 446481..447290 (-) 810 WP_014572051.1 ABC transporter permease -

Sequence


Protein


Download         Length: 129 a.a.        Molecular weight: 14703.77 Da        Isoelectric Point: 6.9865

>NTDB_id=182966 LLJM2_RS02190 WP_021165898.1 436623..437012(+) (ssbB) [Lactococcus cremoris strain JM2]
MNKTMLIGRLTNAPEISKTTNNKSYVRVTLAVNRRFKNEKGEREADFISIILWGKSAETFVSYAKKGSLISVEGEIRTRN
YTDKNEQKHCITEILGLSYDLLESRATLALRESAVNFEELLLEADELPF

Nucleotide


Download         Length: 390 bp        

>NTDB_id=182966 LLJM2_RS02190 WP_021165898.1 436623..437012(+) (ssbB) [Lactococcus cremoris strain JM2]
ATGAATAAAACCATGTTGATTGGTCGTTTAACAAACGCACCTGAAATTTCGAAAACAACGAACAATAAATCTTATGTCCG
TGTGACTCTGGCAGTCAATCGCCGCTTCAAAAATGAAAAAGGAGAGCGAGAAGCAGACTTTATCTCGATAATTTTATGGG
GAAAATCAGCAGAAACATTCGTTTCCTATGCTAAAAAAGGAAGTCTTATTTCTGTAGAGGGAGAGATTAGAACTAGAAAT
TATACTGATAAAAATGAACAGAAACACTGTATTACAGAAATCTTAGGTTTGAGCTATGATTTATTGGAAAGTCGTGCGAC
TCTTGCCTTGAGAGAAAGCGCTGTAAATTTTGAGGAACTCTTACTTGAAGCTGATGAGCTCCCTTTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A166Z4Y5

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbB Lactococcus lactis subsp. cremoris KW2

96.899

100

0.969

  ssbB Streptococcus sobrinus strain NIDR 6715-7

60

100

0.605

  ssbA Streptococcus mutans UA159

56.154

100

0.566

  ssbB/cilA Streptococcus pneumoniae TIGR4

56.154

100

0.566

  ssbB/cilA Streptococcus mitis SK321

55.385

100

0.558

  ssbB/cilA Streptococcus pneumoniae R6

55.385

100

0.558

  ssbB/cilA Streptococcus pneumoniae Rx1

55.385

100

0.558

  ssbB/cilA Streptococcus pneumoniae D39

55.385

100

0.558

  ssbB/cilA Streptococcus mitis NCTC 12261

55.385

100

0.558

  ssb Latilactobacillus sakei subsp. sakei 23K

41.964

86.822

0.364


Multiple sequence alignment