Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssbB   Type   Machinery gene
Locus tag   LLJM1_RS10030 Genome accession   NZ_CP015899
Coordinates   1918589..1918978 (-) Length   129 a.a.
NCBI ID   WP_021165898.1    Uniprot ID   A0A166Z4Y5
Organism   Lactococcus cremoris strain JM1     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1903408..1918964 1918589..1918978 flank -375


Gene organization within MGE regions


Location: 1903408..1918978
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLJM1_RS09945 (LLJM1_1923) - 1903422..1904369 (+) 948 WP_003130410.1 IS30 family transposase -
  LLJM1_RS09950 (LLJM1_1924) mvk 1904599..1905531 (+) 933 WP_014572053.1 mevalonate kinase -
  LLJM1_RS09955 (LLJM1_1925) - 1905662..1906162 (-) 501 WP_015082048.1 MarR family winged helix-turn-helix transcriptional regulator -
  LLJM1_RS09960 (LLJM1_1926) - 1906294..1907223 (+) 930 WP_011675476.1 daunorubicin resistance protein DrrA family ABC transporter ATP-binding protein -
  LLJM1_RS09965 (LLJM1_1927) - 1907236..1908045 (+) 810 WP_014572051.1 ABC transporter permease -
  LLJM1_RS09970 (LLJM1_1928) - 1908120..1908311 (+) 192 WP_032950911.1 hypothetical protein -
  LLJM1_RS09975 (LLJM1_1929) - 1908372..1909307 (-) 936 WP_031559015.1 IS30 family transposase -
  LLJM1_RS09980 (LLJM1_1930) rsmI 1909465..1910340 (-) 876 WP_032950912.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  LLJM1_RS09985 (LLJM1_1931) yabA 1910340..1910666 (-) 327 WP_011675472.1 DNA replication initiation control protein YabA -
  LLJM1_RS09990 (LLJM1_1932) ricT 1910659..1911441 (-) 783 WP_011834464.1 stage 0 sporulation family protein -
  LLJM1_RS09995 (LLJM1_1933) - 1911491..1912351 (-) 861 WP_011675470.1 DNA polymerase III subunit delta' -
  LLJM1_RS10000 (LLJM1_1934) tmk 1912483..1913118 (-) 636 WP_015082045.1 dTMP kinase -
  LLJM1_RS10005 (LLJM1_1935) yycF 1913296..1913997 (+) 702 WP_003131580.1 response regulator YycF -
  LLJM1_RS10010 (LLJM1_1936) - 1913990..1915417 (+) 1428 WP_011675468.1 cell wall metabolism sensor histidine kinase WalK -
  LLJM1_RS10015 (LLJM1_1937) - 1915607..1916419 (+) 813 WP_011675466.1 MBL fold metallo-hydrolase -
  LLJM1_RS10020 (LLJM1_1938) groL 1916471..1918099 (-) 1629 WP_032950921.1 chaperonin GroEL -
  LLJM1_RS10025 (LLJM1_1939) groES 1918186..1918470 (-) 285 WP_011675464.1 co-chaperone GroES -
  LLJM1_RS10030 (LLJM1_1940) ssbB 1918589..1918978 (-) 390 WP_021165898.1 single-stranded DNA-binding protein Machinery gene

Sequence

Protein


Download         Length: 129 a.a.        Molecular weight: 14703.77 Da        Isoelectric Point: 6.9865

>NTDB_id=182948 LLJM1_RS10030 WP_021165898.1 1918589..1918978(-) (ssbB) [Lactococcus cremoris strain JM1]
MNKTMLIGRLTNAPEISKTTNNKSYVRVTLAVNRRFKNEKGEREADFISIILWGKSAETFVSYAKKGSLISVEGEIRTRN
YTDKNEQKHCITEILGLSYDLLESRATLALRESAVNFEELLLEADELPF

Nucleotide


Download         Length: 390 bp        

>NTDB_id=182948 LLJM1_RS10030 WP_021165898.1 1918589..1918978(-) (ssbB) [Lactococcus cremoris strain JM1]
ATGAATAAAACCATGTTGATTGGTCGTTTAACAAACGCACCTGAAATTTCGAAAACAACGAACAATAAATCTTATGTCCG
TGTGACTCTGGCAGTCAATCGCCGCTTCAAAAATGAAAAAGGAGAGCGAGAAGCAGACTTTATCTCGATAATTTTATGGG
GAAAATCAGCAGAAACATTCGTTTCCTATGCTAAAAAAGGAAGTCTTATTTCTGTAGAGGGAGAGATTAGAACTAGAAAT
TATACTGATAAAAATGAACAGAAACACTGTATTACAGAAATCTTAGGTTTGAGCTATGATTTATTGGAAAGTCGTGCGAC
TCTTGCCTTGAGAGAAAGCGCTGTAAATTTTGAGGAACTCTTACTTGAAGCTGATGAGCTCCCTTTCTAA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(1-95)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A166Z4Y5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbB Lactococcus lactis subsp. cremoris KW2

96.899

100

0.969
  ssbB Streptococcus sobrinus strain NIDR 6715-7

60

100

0.605
  ssbA Streptococcus mutans UA159

56.154

100

0.566
  ssbB/cilA Streptococcus pneumoniae TIGR4

56.154

100

0.566
  ssbB/cilA Streptococcus mitis SK321

55.385

100

0.558
  ssbB/cilA Streptococcus pneumoniae R6

55.385

100

0.558
  ssbB/cilA Streptococcus pneumoniae Rx1

55.385

100

0.558
  ssbB/cilA Streptococcus pneumoniae D39

55.385

100

0.558
  ssbB/cilA Streptococcus mitis NCTC 12261

55.385

100

0.558
  ssb Latilactobacillus sakei subsp. sakei 23K

41.964

86.822

0.364

Multiple sequence alignment