Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   A8140_RS13040 Genome accession   NZ_CP015863
Coordinates   2736800..2738023 (+) Length   407 a.a.
NCBI ID   WP_005534044.1    Uniprot ID   -
Organism   Vibrio campbellii CAIM 519 = NBRC 15631 = ATCC 25920 strain ATCC 25920 CAIM 519T     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2731800..2743023
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A8140_RS13020 (A8140_12380) ampD 2732841..2733392 (-) 552 WP_005536692.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  A8140_RS13025 (A8140_12385) nadC 2733485..2734372 (+) 888 WP_005536694.1 carboxylating nicotinate-nucleotide diphosphorylase -
  A8140_RS13030 (A8140_12390) pilA 2734637..2735068 (+) 432 WP_005534039.1 pilin Machinery gene
  A8140_RS13035 (A8140_12395) pilB 2735080..2736765 (+) 1686 WP_005534041.1 type IV-A pilus assembly ATPase PilB Machinery gene
  A8140_RS13040 (A8140_12400) pilC 2736800..2738023 (+) 1224 WP_005534044.1 type II secretion system F family protein Machinery gene
  A8140_RS13045 (A8140_12405) pilD 2738096..2738965 (+) 870 WP_005534046.1 prepilin peptidase Machinery gene
  A8140_RS13050 (A8140_12410) coaE 2738966..2739580 (+) 615 WP_005534049.1 dephospho-CoA kinase -
  A8140_RS13055 (A8140_12415) zapD 2739608..2740348 (+) 741 WP_005534051.1 cell division protein ZapD -
  A8140_RS13060 (A8140_12420) yacG 2740411..2740605 (+) 195 WP_005534053.1 DNA gyrase inhibitor YacG -
  A8140_RS13065 (A8140_12425) rplS 2740942..2741295 (-) 354 WP_005379971.1 50S ribosomal protein L19 -
  A8140_RS13070 (A8140_12430) trmD 2741337..2742080 (-) 744 WP_005534054.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  A8140_RS13075 (A8140_12435) rimM 2742108..2742656 (-) 549 WP_005534055.1 ribosome maturation factor RimM -
  A8140_RS13080 (A8140_12440) rpsP 2742684..2742932 (-) 249 WP_004410028.1 30S ribosomal protein S16 -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 44994.27 Da        Isoelectric Point: 10.3971

>NTDB_id=182595 A8140_RS13040 WP_005534044.1 2736800..2738023(+) (pilC) [Vibrio campbellii CAIM 519 = NBRC 15631 = ATCC 25920 strain ATCC 25920 CAIM 519T]
MKTIAPQLKNYRWKGINSSGKKTSGNVLAMTEIEVRERLDAQHIKIKKLKKGSISFITKISHRVKGKDITIFTRQIATML
MTGVPIVQALKLVSENHKKAEMKSILMSVTRAIEAGTPMSKAMRTASTHFDPLYTDLIATGEQSGNLSQVFERLATYREK
SEQLRAKVIKALIYPAMVVLVALGVSYLMLTRVIPEFEKMFTGFGADLPAFTQMVLNLSAWTQNWGPFIGLSFVSLLISG
KIMSQRSDSFRLLAARSSLRVPILGPVLSKAAIAKFSRTLATSFSAGIPILTALKTTSKTSGNMHYQLAIEEVYRDTAAG
MPMYIAMRNCHVFPELVLQMVMIGEESGRLDDMLNKIAAIYEFEVDNTVDNLSKILEPLIIVFLGVVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=182595 A8140_RS13040 WP_005534044.1 2736800..2738023(+) (pilC) [Vibrio campbellii CAIM 519 = NBRC 15631 = ATCC 25920 strain ATCC 25920 CAIM 519T]
ATGAAAACCATCGCTCCTCAACTCAAGAACTACCGTTGGAAAGGCATTAATAGCTCGGGTAAAAAAACCTCTGGAAACGT
GCTCGCCATGACCGAAATCGAGGTAAGAGAGCGCCTCGATGCTCAACACATCAAGATAAAGAAACTGAAGAAAGGCAGCA
TTTCTTTTATCACCAAGATCAGTCATCGCGTCAAAGGCAAAGACATCACGATATTTACTCGCCAAATTGCCACTATGCTA
ATGACGGGCGTACCGATTGTTCAGGCACTTAAGTTGGTTTCAGAGAACCACAAAAAAGCCGAGATGAAATCGATTCTGAT
GAGTGTCACACGAGCGATAGAGGCAGGGACACCGATGTCGAAAGCCATGCGCACTGCCAGTACACATTTTGATCCGCTTT
ATACAGACCTAATCGCGACTGGTGAGCAGTCCGGTAACCTATCTCAAGTCTTTGAACGTTTGGCAACTTATCGAGAAAAG
AGCGAGCAGTTGCGCGCAAAAGTAATTAAAGCGCTCATTTACCCAGCGATGGTGGTTTTAGTAGCGCTTGGGGTTTCCTA
TTTAATGCTGACTAGAGTTATCCCTGAGTTTGAAAAGATGTTTACAGGTTTTGGAGCCGACTTACCCGCCTTTACTCAGA
TGGTACTCAACCTCTCTGCTTGGACCCAAAACTGGGGCCCCTTTATTGGACTGAGTTTTGTGAGCCTGCTCATTTCCGGC
AAAATTATGTCGCAACGATCCGATTCATTTCGCCTTTTAGCCGCGCGTTCGAGTTTAAGAGTCCCTATCTTGGGGCCTGT
ATTATCCAAAGCAGCCATTGCCAAGTTTAGCCGTACTTTAGCAACCAGCTTTAGTGCGGGTATTCCAATTCTCACCGCAT
TAAAAACAACCTCAAAAACCTCCGGTAATATGCACTATCAATTAGCAATTGAAGAAGTGTATCGAGACACTGCAGCTGGT
ATGCCGATGTATATTGCCATGCGTAACTGTCACGTTTTCCCTGAGCTAGTACTGCAAATGGTCATGATAGGTGAAGAGTC
AGGTCGATTGGATGATATGCTCAACAAGATAGCCGCTATTTATGAGTTTGAAGTGGATAATACCGTTGATAATCTGAGTA
AGATTTTAGAACCATTGATCATCGTCTTTCTTGGCGTGGTGGTTGGCGGTCTAGTCACAGCAATGTACTTACCAATCTTT
AATTTGATGAGCGTATTAGGATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

99.509

100

0.995
  pilC Vibrio cholerae strain A1552

73.75

98.28

0.725
  pilC Acinetobacter baylyi ADP1

42.211

97.789

0.413
  pilC Acinetobacter baumannii D1279779

40.199

99.017

0.398
  pilC Pseudomonas stutzeri DSM 10701

40.657

97.297

0.396
  pilC Legionella pneumophila strain ERS1305867

39.655

99.754

0.396
  pilG Neisseria gonorrhoeae MS11

38.821

100

0.388
  pilG Neisseria meningitidis 44/76-A

37.99

100

0.381

Multiple sequence alignment