Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   A7J50_RS04035 Genome accession   NZ_CP015600
Coordinates   945571..946779 (-) Length   402 a.a.
NCBI ID   WP_064450654.1    Uniprot ID   A0A172YWS9
Organism   Pseudomonas antarctica strain PAMC 27494     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 940571..951779
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A7J50_RS04000 (A7J50_0790) - 941841..942014 (-) 174 WP_064450648.1 DUF3094 family protein -
  A7J50_RS04005 (A7J50_0791) - 942104..942733 (-) 630 WP_053254334.1 DUF1780 domain-containing protein -
  A7J50_RS04010 (A7J50_0792) - 942925..943614 (+) 690 WP_064450649.1 energy-coupling factor ABC transporter permease -
  A7J50_RS04015 (A7J50_0793) - 943679..943894 (+) 216 WP_064450650.1 hypothetical protein -
  A7J50_RS04020 (A7J50_0794) yacG 943891..944091 (-) 201 WP_064450651.1 DNA gyrase inhibitor YacG -
  A7J50_RS04025 (A7J50_0795) coaE 944088..944711 (-) 624 WP_064450652.1 dephospho-CoA kinase -
  A7J50_RS04030 (A7J50_0796) pilD 944708..945574 (-) 867 WP_064450653.1 prepilin peptidase Machinery gene
  A7J50_RS04035 (A7J50_0797) pilC 945571..946779 (-) 1209 WP_064450654.1 type II secretion system F family protein Machinery gene
  A7J50_RS04040 (A7J50_0798) - 946982..947395 (+) 414 WP_064450655.1 pilin -
  A7J50_RS04050 (A7J50_0799) - 947687..947992 (+) 306 WP_064450656.1 DUF6388 family protein -
  A7J50_RS04055 (A7J50_0800) nadC 948053..948901 (-) 849 WP_064450657.1 carboxylating nicotinate-nucleotide diphosphorylase -
  A7J50_RS04060 (A7J50_0801) - 949147..951321 (+) 2175 WP_064450658.1 DUF1631 domain-containing protein -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43396.89 Da        Isoelectric Point: 9.9314

>NTDB_id=181277 A7J50_RS04035 WP_064450654.1 945571..946779(-) (pilC) [Pseudomonas antarctica strain PAMC 27494]
MNEAATIYAWEGINRKGRRVSGQTAGHNLALIKAQLRQQGICPGRVRKKTSHLPSFAPPVKPADIALFTRQLATLLKAGI
PLLQAFDIISEGFEHRQMRELIKGLKQEIAAGNNLAAALRKQPRYFDELYCNLIAAGEQAGALETLLERVAVHLEKSQQL
KARIKKAMTYPCAVIVVACVVSAILLIHVVPQFQSLFSGVDGELPGFTLKVIALSEFLQHAWWMVALGLGATIAGLRQAY
RASPGFRHGLDSGLLKVPLAGTLLRKSAVARYARTLSTTFAAGVPLVQALDSVAGATGNGLFKKAIEQMRQDVSTGMQLN
HSMAHSGLFPGMAIQMTAIGEESGTLDRMLEKVAQHFEADVENLVDNLTSLMEPLIMVILGGIVGALVIAMYLPVFQLGS
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=181277 A7J50_RS04035 WP_064450654.1 945571..946779(-) (pilC) [Pseudomonas antarctica strain PAMC 27494]
ATGAATGAAGCTGCAACGATCTACGCCTGGGAAGGCATCAACCGCAAAGGGCGCAGGGTCTCAGGGCAAACCGCAGGCCA
TAACCTTGCGCTGATCAAGGCGCAACTGCGCCAGCAAGGAATCTGCCCCGGCCGCGTGCGCAAAAAAACCTCGCACCTGC
CAAGCTTTGCGCCGCCAGTAAAACCGGCAGATATTGCCCTGTTCACCCGCCAACTGGCGACATTGCTGAAGGCCGGCATA
CCGCTGCTGCAAGCCTTCGACATCATCAGCGAAGGCTTCGAACACCGACAGATGCGCGAGCTGATAAAGGGCTTGAAACA
GGAGATCGCCGCCGGGAACAACCTGGCGGCAGCACTGCGCAAACAACCGCGCTACTTCGATGAACTGTATTGCAACCTGA
TCGCTGCGGGCGAACAGGCCGGTGCCCTGGAAACCTTGCTGGAGCGGGTGGCGGTACACCTGGAAAAAAGCCAGCAGCTC
AAGGCGCGAATCAAAAAGGCCATGACCTACCCCTGCGCAGTCATCGTGGTCGCCTGCGTGGTCAGTGCAATCCTGCTCAT
CCACGTGGTACCGCAGTTTCAGAGCCTTTTTAGCGGGGTCGACGGCGAGCTGCCAGGCTTTACCTTGAAGGTCATTGCCC
TGTCCGAATTCCTGCAACACGCCTGGTGGATGGTCGCGCTGGGCCTGGGCGCTACCATTGCCGGGCTGCGCCAGGCTTAT
CGGGCTTCCCCCGGTTTTCGTCACGGCCTCGATAGCGGTTTGTTGAAGGTGCCCCTGGCAGGCACACTGCTGAGAAAATC
CGCCGTTGCCCGCTACGCCCGTACACTTTCAACGACTTTTGCAGCGGGCGTGCCGTTGGTGCAGGCCCTGGACTCCGTGG
CGGGTGCGACAGGCAATGGTTTGTTCAAAAAGGCGATCGAACAGATGCGTCAAGACGTATCCACAGGCATGCAGTTGAAT
CACTCCATGGCGCATAGCGGCCTGTTCCCCGGCATGGCGATCCAGATGACAGCGATCGGTGAAGAATCGGGCACCCTGGA
CCGCATGCTGGAAAAAGTTGCCCAGCATTTTGAGGCCGACGTGGAGAATCTGGTCGACAACCTCACCAGCCTGATGGAGC
CGCTCATCATGGTGATATTGGGGGGCATTGTCGGTGCATTGGTGATCGCCATGTACCTGCCGGTCTTCCAGCTGGGGTCG
GCATTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(273-394)

Type II secretion system (T2SS), protein F (T2SSF)

(68-191)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A172YWS9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

60.705

98.756

0.599
  pilC Acinetobacter baylyi ADP1

53.788

98.507

0.53
  pilC Acinetobacter baumannii D1279779

52.658

98.259

0.517
  pilC Legionella pneumophila strain ERS1305867

49.622

98.756

0.49
  pilC Vibrio cholerae strain A1552

39.798

98.756

0.393
  pilG Neisseria gonorrhoeae MS11

40.838

95.025

0.388
  pilG Neisseria meningitidis 44/76-A

40.052

95.025

0.381
  pilC Vibrio campbellii strain DS40M4

38.636

98.507

0.381
  pilC Thermus thermophilus HB27

37.343

99.254

0.371

Multiple sequence alignment