Detailed information NTG0181276

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: A7J50_RS04030
• Genome accession: NZ_CP015600
• Coordinates: 944708..945574 (-)
• Length: 288 a.a.
• NCBI ID: WP_064450653.1
• Uniprot ID: A0A172YVU7
• Organism: Pseudomonas antarctica strain PAMC 27494
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 939708..950574

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A7J50_RS03995 (A7J50_0789)	-	940482..941780 (-)	1299	WP_064450647.1	NAD(P)/FAD-dependent oxidoreductase	-
A7J50_RS04000 (A7J50_0790)	-	941841..942014 (-)	174	WP_064450648.1	DUF3094 family protein	-
A7J50_RS04005 (A7J50_0791)	-	942104..942733 (-)	630	WP_053254334.1	DUF1780 domain-containing protein	-
A7J50_RS04010 (A7J50_0792)	-	942925..943614 (+)	690	WP_064450649.1	energy-coupling factor ABC transporter permease	-
A7J50_RS04015 (A7J50_0793)	-	943679..943894 (+)	216	WP_064450650.1	hypothetical protein	-
A7J50_RS04020 (A7J50_0794)	yacG	943891..944091 (-)	201	WP_064450651.1	DNA gyrase inhibitor YacG	-
A7J50_RS04025 (A7J50_0795)	coaE	944088..944711 (-)	624	WP_064450652.1	dephospho-CoA kinase	-
A7J50_RS04030 (A7J50_0796)	pilD	944708..945574 (-)	867	WP_064450653.1	prepilin peptidase	Machinery gene
A7J50_RS04035 (A7J50_0797)	pilC	945571..946779 (-)	1209	WP_064450654.1	type II secretion system F family protein	Machinery gene
A7J50_RS04040 (A7J50_0798)	-	946982..947395 (+)	414	WP_064450655.1	pilin	-
A7J50_RS04050 (A7J50_0799)	-	947687..947992 (+)	306	WP_064450656.1	DUF6388 family protein	-
A7J50_RS04055 (A7J50_0800)	nadC	948053..948901 (-)	849	WP_064450657.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 31925.03 Da
• Isoelectric Point: 7.6034

>NTDB_id=181276 A7J50_RS04030 WP_064450653.1 944708..945574(-) (pilD) [Pseudomonas antarctica strain PAMC 27494]
MSLLPSEQSWVFIGMALALGLIVGSFINVLVWRLPKMLEREWRAQAQEVLGLPADRANPTYNLIHPNSCCPHCDQPIRAW
ENIPLLSYLILRGRCAHCREPISSRYPITELACALGSALVAWHFGFGWQAGAVMVLSWGLLAMSLIDVDHQLLPDVLVLP
LLWLGLILNSFDVLTTLPNALWGAVIGYMSLWTVFWLFKLITGKDGMGYGDFKLLALLGAWGGWQILPMTLLISSLLGVL
AGLILMRLRRSQASIPMPFGPCLAIAGWIALLWGGQITDFYLQSVGFR

Nucleotide

• Length: 867 bp

>NTDB_id=181276 A7J50_RS04030 WP_064450653.1 944708..945574(-) (pilD) [Pseudomonas antarctica strain PAMC 27494]
TTGAGCCTGCTGCCCAGCGAACAATCATGGGTCTTTATCGGGATGGCGCTCGCTCTGGGCCTGATCGTCGGCAGCTTCAT
CAACGTGCTGGTGTGGCGTCTGCCAAAGATGCTCGAGCGCGAATGGCGTGCCCAAGCCCAGGAAGTGCTGGGCTTGCCTG
CCGATCGCGCGAACCCCACTTACAACCTGATACACCCCAACTCCTGCTGCCCGCATTGCGACCAGCCGATTCGTGCCTGG
GAAAATATCCCGCTGCTCAGCTACCTGATACTCAGGGGCCGTTGCGCCCATTGTCGTGAACCTATCAGTAGCCGCTACCC
CATCACCGAACTGGCCTGCGCGCTGGGCTCAGCTCTGGTTGCCTGGCATTTTGGTTTCGGTTGGCAAGCTGGCGCGGTGA
TGGTGTTGAGCTGGGGGTTGCTGGCCATGAGCCTGATCGACGTGGATCATCAACTGTTGCCGGACGTTCTGGTGTTGCCG
CTGCTATGGCTCGGGCTGATCCTCAATAGCTTCGACGTGTTGACGACGCTGCCCAATGCGCTGTGGGGCGCAGTCATAGG
CTATATGAGCCTGTGGACGGTGTTCTGGCTGTTCAAGCTGATCACCGGCAAGGACGGCATGGGCTATGGCGACTTCAAGT
TATTGGCCCTGCTCGGTGCCTGGGGTGGTTGGCAGATTCTGCCGATGACGCTACTGATCTCATCGCTGCTAGGCGTGCTC
GCCGGCTTGATCCTGATGCGCTTGCGCAGGTCCCAGGCTTCGATACCGATGCCGTTTGGCCCGTGCCTGGCAATTGCCGG
CTGGATTGCATTGCTCTGGGGTGGTCAAATAACCGACTTCTATTTGCAGTCTGTCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (134-243)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (19-124)

3D structure

• AlphaFold DB: A0A172YVU7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	52.33	96.875	0.507
pilD	Acinetobacter baumannii D1279779	49.818	95.486	0.476
pilD	Acinetobacter nosocomialis M2	49.635	95.139	0.472
pilD	Vibrio campbellii strain DS40M4	48.571	97.222	0.472
pilD	Neisseria gonorrhoeae MS11	48.45	89.583	0.434