Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   A4W74_RS03685 Genome accession   NZ_CP015494
Coordinates   715514..716389 (+) Length   291 a.a.
NCBI ID   WP_111447555.1    Uniprot ID   A0AAJ5UP63
Organism   Latilactobacillus curvatus strain TMW 1.1390     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 710514..721389
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4W74_RS03645 (A4W74_03625) - 710619..711446 (-) 828 WP_054644033.1 LysR family transcriptional regulator -
  A4W74_RS03650 (A4W74_03630) - 711555..712568 (+) 1014 WP_253733849.1 YeiH family protein -
  A4W74_RS03655 (A4W74_03635) - 712659..712898 (-) 240 WP_004270892.1 cytochrome b5 domain-containing protein -
  A4W74_RS03660 (A4W74_03640) - 713093..713428 (+) 336 WP_136903898.1 hypothetical protein -
  A4W74_RS03670 (A4W74_03650) - 713609..713986 (-) 378 WP_111447553.1 hypothetical protein -
  A4W74_RS03675 (A4W74_03655) - 714089..714589 (+) 501 WP_004270893.1 VanZ family protein -
  A4W74_RS03680 (A4W74_03660) - 714679..715410 (+) 732 WP_004270897.1 YebC/PmpR family DNA-binding transcriptional regulator -
  A4W74_RS03685 (A4W74_03665) comGA 715514..716389 (+) 876 WP_111447555.1 competence type IV pilus ATPase ComGA Machinery gene
  A4W74_RS03690 (A4W74_03670) comGB 716379..717380 (+) 1002 WP_004270906.1 type II secretion system F family protein Machinery gene
  A4W74_RS03695 (A4W74_03675) comGC 717377..717685 (+) 309 WP_004270901.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  A4W74_RS03700 (A4W74_03680) - 717717..718109 (+) 393 WP_004270899.1 hypothetical protein -
  A4W74_RS03705 (A4W74_03685) - 718096..718401 (+) 306 WP_035186736.1 hypothetical protein -
  A4W74_RS10420 - 718367..718450 (+) 84 Protein_712 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  A4W74_RS03710 (A4W74_03690) - 718472..718852 (+) 381 WP_004270914.1 ComGF family competence protein -
  A4W74_RS03715 (A4W74_03695) - 718830..719153 (+) 324 WP_039099486.1 hypothetical protein -
  A4W74_RS03720 (A4W74_03700) - 719269..720288 (+) 1020 WP_253733850.1 class I SAM-dependent methyltransferase -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32042.84 Da        Isoelectric Point: 5.5686

>NTDB_id=180046 A4W74_RS03685 WP_111447555.1 715514..716389(+) (comGA) [Latilactobacillus curvatus strain TMW 1.1390]
MTEVLTTLLQKCATEKAQDLYFRPLSTGWAIIERLATGLKPLQKVTLSEGNQWLNQMKYQGGMDISETRRPQTGRSAIEV
AGELIYLRLATVGDFLNRESLVVRFIYPIGQTYHCDDSQILSRLMQLIQQPGLLLFAGPTGSGKTTSLYYLAEQVMQDKM
VVAIEDPIEIQQPAFLQLQVNEAAGLTYAALLKISLRLRPDTLIIGEIRDLQTAQHAVSAALSGHLVLSTIHARSTTGVM
DRLLDLGISHSQLTACLAGVAYQSLVVEGHNVAAHYELATAQQLEEAVHED

Nucleotide


Download         Length: 876 bp        

>NTDB_id=180046 A4W74_RS03685 WP_111447555.1 715514..716389(+) (comGA) [Latilactobacillus curvatus strain TMW 1.1390]
ATGACCGAAGTATTAACAACCCTTTTGCAAAAGTGCGCAACGGAAAAAGCGCAGGATCTTTATTTTCGACCACTATCGAC
GGGTTGGGCAATTATTGAACGCTTAGCGACAGGACTAAAGCCGTTACAAAAAGTTACTCTGTCAGAAGGCAATCAATGGC
TCAATCAAATGAAGTATCAAGGGGGGATGGATATCAGTGAAACTCGGCGTCCACAGACCGGCCGTTCAGCGATCGAGGTG
GCAGGCGAATTGATTTATTTACGGTTGGCAACCGTGGGGGATTTTTTGAATCGGGAAAGTTTAGTTGTTCGTTTCATTTA
TCCCATTGGGCAGACTTATCATTGCGACGACTCGCAGATTTTAAGTCGTTTAATGCAGTTAATTCAGCAACCAGGATTAT
TGCTATTTGCCGGTCCAACTGGTTCAGGGAAGACAACAAGTTTGTACTATCTTGCGGAACAAGTCATGCAAGATAAGATG
GTGGTTGCGATTGAAGATCCGATAGAGATTCAACAGCCAGCTTTCTTACAGTTACAGGTGAATGAAGCCGCAGGGTTAAC
CTATGCAGCATTGTTGAAAATCAGTTTGCGATTACGGCCTGATACCTTAATTATTGGTGAGATTCGCGACTTACAGACGG
CCCAACACGCGGTTTCTGCGGCGTTGAGCGGTCACTTGGTACTAAGTACGATTCACGCGCGAAGTACAACGGGGGTGATG
GACCGTCTATTAGATCTTGGAATTAGCCACTCACAATTAACCGCTTGTTTAGCGGGTGTTGCCTATCAATCACTAGTGGT
CGAAGGCCACAATGTAGCGGCCCACTATGAGTTAGCAACTGCACAACAATTGGAGGAGGCTGTTCATGAAGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Latilactobacillus sakei subsp. sakei 23K

69.534

95.876

0.667
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

42.647

93.471

0.399
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

42.222

92.784

0.392
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

41.199

91.753

0.378
  comGA/cglA/cilD Streptococcus pneumoniae R6

41.199

91.753

0.378
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

41.199

91.753

0.378
  comGA/cglA/cilD Streptococcus pneumoniae D39

41.199

91.753

0.378
  comGA Lactococcus lactis subsp. cremoris KW2

41.221

90.034

0.371
  comYA Streptococcus mutans UA140

36.77

100

0.368
  comYA Streptococcus mutans UA159

36.77

100

0.368
  comYA Streptococcus gordonii str. Challis substr. CH1

38.828

93.814

0.364

Multiple sequence alignment