Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   A4W73_RS03655 Genome accession   NZ_CP015493
Coordinates   705681..706556 (+) Length   291 a.a.
NCBI ID   WP_076786756.1    Uniprot ID   -
Organism   Latilactobacillus curvatus strain TMW 1.1381     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 700681..711556
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4W73_RS03615 (A4W73_03605) - 700787..701614 (-) 828 WP_076789371.1 LysR family transcriptional regulator -
  A4W73_RS03620 (A4W73_03610) - 701723..702736 (+) 1014 WP_081394496.1 YeiH family protein -
  A4W73_RS03625 (A4W73_03615) - 702827..703066 (-) 240 WP_004270892.1 cytochrome b5 domain-containing protein -
  A4W73_RS03630 (A4W73_03620) - 703260..703595 (+) 336 WP_076786752.1 hypothetical protein -
  A4W73_RS03640 (A4W73_03630) - 703776..704153 (-) 378 WP_039099519.1 hypothetical protein -
  A4W73_RS03645 (A4W73_03635) - 704256..704756 (+) 501 WP_076789374.1 VanZ family protein -
  A4W73_RS03650 (A4W73_03640) - 704846..705577 (+) 732 WP_004270897.1 YebC/PmpR family DNA-binding transcriptional regulator -
  A4W73_RS03655 (A4W73_03645) comGA 705681..706556 (+) 876 WP_076786756.1 competence type IV pilus ATPase ComGA Machinery gene
  A4W73_RS03660 (A4W73_03650) comGB 706546..707547 (+) 1002 WP_076786758.1 type II secretion system F family protein Machinery gene
  A4W73_RS03665 (A4W73_03655) comGC 707544..707852 (+) 309 WP_004270901.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  A4W73_RS03670 (A4W73_03660) - 707884..708276 (+) 393 WP_004270899.1 hypothetical protein -
  A4W73_RS03675 (A4W73_03665) - 708341..708568 (+) 228 WP_158070299.1 hypothetical protein -
  A4W73_RS10180 - 708534..708617 (+) 84 Protein_707 type II secretion system protein -
  A4W73_RS03680 (A4W73_03670) - 708639..709019 (+) 381 WP_004270914.1 ComGF family competence protein -
  A4W73_RS03685 (A4W73_03675) - 708997..709320 (+) 324 WP_039099486.1 hypothetical protein -
  A4W73_RS03690 (A4W73_03680) - 709436..710455 (+) 1020 WP_311318493.1 class I SAM-dependent methyltransferase -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32047.86 Da        Isoelectric Point: 5.3292

>NTDB_id=180014 A4W73_RS03655 WP_076786756.1 705681..706556(+) (comGA) [Latilactobacillus curvatus strain TMW 1.1381]
MTEVLTTLLQKCATEKAQDLYFRPLSTGWAIIERLATGLKPLQKVTLSEGNQWLNQMKYQAGMDISETRRPQTGRSAIEV
AGELIYLRLATVGDFLNRESLVVRFIYPIGQTYHCDDSQILSRLMQLIQQPGLLLFAGPTGSGKTTSLYYLAEQVMQDKM
VVAIEDPIEIQQPAFLQLQVNEAAGLTYAALLKISLRLRPDTLIIGEIRDLQTAQHAVSAALSGHLVLSTIHARSTTGVM
DRLLDLGISHSQLTACLAGVAYQSLVVEGQNVAAHYELATAQQLEEAVHED

Nucleotide


Download         Length: 876 bp        

>NTDB_id=180014 A4W73_RS03655 WP_076786756.1 705681..706556(+) (comGA) [Latilactobacillus curvatus strain TMW 1.1381]
ATGACCGAAGTATTAACAACCCTTTTGCAAAAGTGCGCAACGGAAAAAGCGCAGGATCTTTATTTTCGACCACTATCGAC
GGGTTGGGCAATTATTGAACGCTTAGCGACAGGACTAAAGCCGTTACAAAAAGTTACTCTGTCAGAAGGCAATCAATGGC
TCAATCAAATGAAGTATCAAGCGGGGATGGATATCAGTGAAACTCGGCGTCCACAGACCGGCCGTTCAGCGATCGAGGTG
GCAGGCGAATTGATTTATTTACGGTTGGCAACCGTGGGGGATTTTTTGAATCGGGAAAGTTTAGTTGTTCGTTTCATTTA
TCCCATTGGGCAGACTTATCATTGCGACGACTCGCAGATTTTAAGTCGTTTAATGCAGTTAATTCAGCAACCAGGATTAT
TGCTATTTGCCGGTCCAACTGGTTCAGGGAAGACAACAAGTTTGTACTATCTTGCGGAACAAGTCATGCAAGATAAGATG
GTGGTTGCGATTGAAGATCCGATAGAGATTCAACAGCCAGCGTTCTTACAGTTACAGGTGAATGAAGCCGCAGGGTTAAC
CTATGCAGCATTGTTGAAAATCAGTCTGCGATTACGGCCTGATACCTTAATTATTGGTGAGATTCGCGACTTACAGACGG
CCCAACACGCGGTTTCAGCGGCGTTGAGCGGTCACTTGGTACTAAGTACGATTCACGCGCGAAGTACAACGGGGGTGATG
GACCGTCTATTAGATCTTGGAATTAGCCACTCACAATTAACCGCTTGTTTAGCGGGTGTTGCCTATCAATCACTAGTGGT
CGAAGGCCAAAATGTAGCGGCCCACTATGAGTTAGCAACTGCACAACAATTGGAGGAGGCTGTTCATGAAGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Latilactobacillus sakei subsp. sakei 23K

69.892

95.876

0.67
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

43.015

93.471

0.402
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

42.593

92.784

0.395
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

41.573

91.753

0.381
  comGA/cglA/cilD Streptococcus pneumoniae R6

41.573

91.753

0.381
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

41.573

91.753

0.381
  comGA/cglA/cilD Streptococcus pneumoniae D39

41.573

91.753

0.381
  comGA Lactococcus lactis subsp. cremoris KW2

41.603

90.034

0.375
  comYA Streptococcus mutans UA140

37.113

100

0.371
  comYA Streptococcus mutans UA159

37.113

100

0.371
  comYA Streptococcus gordonii str. Challis substr. CH1

39.194

93.814

0.368

Multiple sequence alignment