Detailed information    

insolico Bioinformatically predicted

Overview

Name   cinA   Type   Machinery gene
Locus tag   A4265_RS08350 Genome accession   NZ_CP015238
Coordinates   1676127..1677398 (-) Length   423 a.a.
NCBI ID   WP_063632178.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain NS53     
Function   require for competence (predicted from homology)   
Unclear

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1666404..1697734 1676127..1677398 within 0


Gene organization within MGE regions


Location: 1666404..1697734
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4265_RS08300 (A4265_07885) nrdG 1666404..1667018 (-) 615 WP_002982219.1 anaerobic ribonucleoside-triphosphate reductase activating protein -
  A4265_RS08305 (A4265_07890) - 1667033..1667527 (-) 495 WP_063632176.1 GNAT family N-acetyltransferase -
  A4265_RS08310 (A4265_07895) - 1667536..1668471 (-) 936 WP_063632177.1 Gfo/Idh/MocA family protein -
  A4265_RS09040 - 1668500..1668646 (-) 147 WP_002982210.1 hypothetical protein -
  A4265_RS08315 (A4265_07900) nrdD 1668828..1671026 (-) 2199 WP_002992831.1 anaerobic ribonucleoside-triphosphate reductase -
  A4265_RS08320 (A4265_07905) - 1671123..1672682 (-) 1560 WP_011285264.1 membrane protein -
  A4265_RS08325 (A4265_07910) - 1673096..1673401 (-) 306 WP_002982199.1 DUF1292 domain-containing protein -
  A4265_RS08330 (A4265_07915) ruvX 1673413..1673832 (-) 420 WP_002982196.1 Holliday junction resolvase RuvX -
  A4265_RS08335 (A4265_07920) - 1673829..1674098 (-) 270 WP_002982194.1 IreB family regulatory phosphoprotein -
  A4265_RS08340 (A4265_07925) spx 1674213..1674611 (-) 399 WP_002982188.1 transcriptional regulator Spx -
  A4265_RS08345 (A4265_07930) recA 1674902..1676038 (-) 1137 WP_002992179.1 recombinase RecA Machinery gene
  A4265_RS08350 (A4265_07935) cinA 1676127..1677398 (-) 1272 WP_063632178.1 competence/damage-inducible protein A Machinery gene
  A4265_RS08355 (A4265_07940) - 1677467..1678037 (-) 571 Protein_1568 DNA-3-methyladenine glycosylase I -
  A4265_RS08360 (A4265_07945) ruvA 1678047..1678643 (-) 597 WP_002992186.1 Holliday junction branch migration protein RuvA -
  A4265_RS08365 (A4265_07950) - 1678645..1679865 (-) 1221 WP_002991361.1 MDR family MFS transporter -
  A4265_RS08370 (A4265_07955) mutL 1679876..1681858 (-) 1983 WP_023610727.1 DNA mismatch repair endonuclease MutL -
  A4265_RS08375 (A4265_07960) - 1681953..1683098 (-) 1146 WP_063629731.1 tyrosine-type recombinase/integrase -
  A4265_RS08380 (A4265_07965) dinD 1683354..1684208 (-) 855 WP_047236050.1 DNA damage-inducible protein D -
  A4265_RS08385 (A4265_07975) - 1685271..1686038 (-) 768 WP_003058809.1 helix-turn-helix transcriptional regulator -
  A4265_RS08390 (A4265_07980) - 1686192..1686398 (+) 207 WP_002992503.1 helix-turn-helix transcriptional regulator -
  A4265_RS08395 (A4265_07985) - 1686432..1687199 (+) 768 WP_076612478.1 BRO family protein -
  A4265_RS08400 (A4265_07990) - 1687209..1687832 (+) 624 WP_063632180.1 Bro-N domain-containing protein -
  A4265_RS08405 (A4265_07995) - 1687859..1688101 (+) 243 WP_000648623.1 hypothetical protein -
  A4265_RS09045 - 1688360..1688530 (+) 171 WP_021775540.1 hypothetical protein -
  A4265_RS08410 (A4265_08000) - 1688754..1688927 (+) 174 WP_000074663.1 plasmid mobilization protein -
  A4265_RS08415 (A4265_08005) - 1689013..1689519 (+) 507 WP_063629729.1 hypothetical protein -
  A4265_RS08420 (A4265_08010) - 1689685..1689963 (+) 279 WP_136282179.1 hypothetical protein -
  A4265_RS08425 (A4265_08015) - 1689950..1690171 (+) 222 WP_010922763.1 hypothetical protein -
  A4265_RS08430 (A4265_08020) - 1690174..1690365 (+) 192 WP_010922764.1 hypothetical protein -
  A4265_RS08435 (A4265_08025) - 1690377..1690706 (+) 330 WP_063629727.1 hypothetical protein -
  A4265_RS08440 (A4265_08030) - 1690709..1690981 (+) 273 WP_001100459.1 hypothetical protein -
  A4265_RS08445 (A4265_08035) - 1690982..1691848 (+) 867 WP_063632182.1 primase alpha helix C-terminal domain-containing protein -
  A4265_RS08450 (A4265_08040) - 1691860..1693254 (+) 1395 WP_063629725.1 VapE domain-containing protein -
  A4265_RS08455 (A4265_08045) - 1693548..1693802 (+) 255 WP_001069294.1 hypothetical protein -
  A4265_RS08460 (A4265_08900) - 1693799..1693972 (+) 174 WP_000694577.1 hypothetical protein -
  A4265_RS09050 - 1693978..1694151 (+) 174 WP_164972313.1 hypothetical protein -
  A4265_RS08465 (A4265_08050) - 1694153..1694662 (+) 510 WP_014407945.1 hypothetical protein -
  A4265_RS08470 (A4265_08055) - 1694737..1695225 (+) 489 WP_063629723.1 hypothetical protein -
  A4265_RS08480 (A4265_08060) - 1695629..1695991 (+) 363 WP_020905578.1 DUF1492 domain-containing protein -
  A4265_RS08485 (A4265_08065) - 1695966..1696334 (+) 369 WP_063632183.1 ArpU family phage packaging/lysis transcriptional regulator -
  A4265_RS08490 (A4265_08070) - 1696378..1696563 (+) 186 WP_009880486.1 hypothetical protein -
  A4265_RS08495 (A4265_08075) - 1696702..1696884 (+) 183 WP_012560491.1 hypothetical protein -
  A4265_RS08500 (A4265_08080) - 1697042..1697734 (+) 693 WP_012560490.1 DUF3800 domain-containing protein -

Sequence

Protein


Download         Length: 423 a.a.        Molecular weight: 45950.67 Da        Isoelectric Point: 4.8322

>NTDB_id=177923 A4265_RS08350 WP_063632178.1 1676127..1677398(-) (cinA) [Streptococcus pyogenes strain NS53]
MKAELIAVGTEILTGQIVNTNAQFLSEKMAELGIDVYFQTAVGDNEERLLSVITTASQRSDLVILCGGLGPTKDDLTKQT
LAKYLRKDLVYDEQACQKLDDFFAKRKPSSRTPNNERQAQVIEGSIPLPNKTGLAVGGFITVDGISYVVLPGPPSELKSM
VNEELVPLLSKQYSTLYSKVLRFFGIGESQLVTVLSDFIENQTDPTIAPYAKTGEVTLRLSTKTENQALADKKLGQLEAQ
LLSRKTLEGQPLADVFYGYGEDNSLARETFELLVKYDKTITAAESLTAGLFQSTLASFPGASQVFNGGFVAYSMEEKAKM
LGLPLEELKSHGVVSAYTAEGMAEQARLLTGADIGVSLTGVAGPDMLEEQPAGTVFIGLATQNKVESIKVLISGRSRLDV
RYIATLHAFNMVRKTLLKLENLL

Nucleotide


Download         Length: 1272 bp        

>NTDB_id=177923 A4265_RS08350 WP_063632178.1 1676127..1677398(-) (cinA) [Streptococcus pyogenes strain NS53]
ATGAAAGCTGAACTGATTGCAGTAGGTACCGAAATTTTGACTGGTCAAATTGTGAATACCAATGCTCAATTTCTGTCGGA
AAAAATGGCAGAGCTAGGTATTGATGTCTATTTTCAAACGGCTGTTGGGGACAACGAGGAGCGTTTACTTTCAGTGATTA
CAACTGCTAGTCAGCGTAGTGACTTGGTGATTTTATGTGGTGGCCTTGGTCCAACGAAAGATGATTTAACCAAACAAACT
TTAGCAAAGTACCTTAGGAAAGACTTGGTTTATGATGAGCAAGCTTGTCAGAAACTAGATGACTTTTTTGCTAAGCGCAA
GCCTTCATCACGGACACCAAATAATGAGCGACAGGCACAAGTGATTGAAGGGTCAATCCCTTTGCCAAATAAAACTGGTC
TTGCGGTTGGTGGGTTCATCACAGTCGATGGTATTAGTTATGTTGTCTTACCGGGTCCTCCAAGTGAATTGAAGTCGATG
GTAAATGAAGAATTGGTACCACTTCTGTCAAAACAATACAGTACATTGTATTCAAAGGTACTACGCTTTTTTGGTATTGG
GGAAAGTCAGTTGGTAACGGTCTTGTCAGATTTTATTGAGAATCAAACTGATCCAACCATTGCTCCGTATGCTAAGACTG
GCGAAGTGACTCTTCGCTTATCAACAAAAACTGAAAACCAAGCTCTGGCAGATAAAAAGTTAGGTCAGCTAGAAGCGCAG
CTACTATCCCGAAAAACTCTTGAAGGTCAACCCTTAGCTGATGTCTTTTATGGCTATGGGGAGGATAATTCCTTAGCGCG
TGAGACATTTGAGCTCTTAGTAAAATATGATAAGACAATTACAGCAGCAGAAAGTCTAACCGCGGGATTATTTCAGTCAA
CTTTGGCGAGTTTTCCAGGAGCTTCTCAAGTATTCAATGGAGGCTTTGTGGCTTATAGCATGGAAGAAAAAGCGAAAATG
CTAGGCCTTCCTTTAGAGGAGTTGAAATCGCATGGCGTTGTTAGTGCTTATACAGCCGAGGGGATGGCGGAGCAAGCAAG
GCTATTGACTGGTGCTGATATTGGGGTAAGTTTAACAGGTGTTGCCGGACCAGATATGTTGGAGGAACAGCCTGCAGGTA
CAGTTTTCATTGGTCTTGCCACTCAAAATAAGGTAGAATCAATAAAGGTTTTAATTAGCGGGCGAAGTCGTTTGGATGTG
CGCTATATCGCTACTTTACATGCCTTTAATATGGTCCGTAAAACTTTATTAAAACTTGAGAATTTGCTATAA

Domains

Predicted by InterproScan.

Probable molybdopterin binding domain (MoCF_biosynth)

(5-170)

Damage-inducible protein CinA KH domain (CinA_KH)

(178-243)

Competence-damaged protein (CinA)

(263-416)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  cinA Streptococcus mutans UA159

69.267

100

0.693
  cinA Streptococcus mitis SK321

69.544

98.582

0.686
  cinA Streptococcus mitis NCTC 12261

69.065

98.582

0.681
  cinA Streptococcus pneumoniae TIGR4

68.106

98.582

0.671
  cinA Streptococcus pneumoniae Rx1

68.106

98.582

0.671
  cinA Streptococcus pneumoniae R6

68.106

98.582

0.671
  cinA Streptococcus pneumoniae D39

67.866

98.582

0.669
  cinA Streptococcus suis isolate S10

53.125

98.345

0.522
  cinA Bacillus subtilis subsp. subtilis str. 168

46.411

98.818

0.459

Multiple sequence alignment