Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ATSB10_RS10355 Genome accession   NZ_CP014841
Coordinates   2260735..2261997 (+) Length   420 a.a.
NCBI ID   WP_063672587.1    Uniprot ID   A0A160N2F2
Organism   Dyella thiooxydans strain ATSB10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2255735..2266997
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ATSB10_RS10340 (ATSB10_21200) - 2256610..2257776 (-) 1167 WP_063672581.1 glycosyltransferase family 4 protein -
  ATSB10_RS10345 (ATSB10_21210) - 2257825..2258712 (-) 888 WP_063672583.1 glycosyltransferase family 2 protein -
  ATSB10_RS10350 (ATSB10_21230) pilB 2258874..2260601 (+) 1728 WP_063672585.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ATSB10_RS10355 (ATSB10_21240) pilC 2260735..2261997 (+) 1263 WP_063672587.1 type II secretion system F family protein Machinery gene
  ATSB10_RS10360 (ATSB10_21250) - 2262071..2262931 (+) 861 WP_063672589.1 A24 family peptidase -
  ATSB10_RS10365 (ATSB10_21260) coaE 2262939..2263544 (+) 606 WP_063672591.1 dephospho-CoA kinase -
  ATSB10_RS10370 (ATSB10_21270) - 2263549..2263947 (-) 399 WP_063672593.1 NUDIX domain-containing protein -
  ATSB10_RS10375 (ATSB10_21280) - 2263947..2264435 (-) 489 WP_063672595.1 dihydrofolate reductase -
  ATSB10_RS10380 (ATSB10_21290) - 2264574..2265368 (-) 795 WP_063672597.1 thymidylate synthase -
  ATSB10_RS10385 (ATSB10_21310) - 2265522..2266538 (+) 1017 WP_063672599.1 TIGR00266 family protein -

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 45587.80 Da        Isoelectric Point: 10.1768

>NTDB_id=174609 ATSB10_RS10355 WP_063672587.1 2260735..2261997(+) (pilC) [Dyella thiooxydans strain ATSB10]
MATATAKNPRDLGAQRAQVSQATVYDWVALDKRGKRMKGEMPAKNASLVKAELRRQGMNPQTVKERGKPLFGATGSTVKP
RDVAIFSRQIATMMASGVPMVQSFEIIADGQKNVRFKNILTDVKQGIEGGASLNEALAKYPIQFDELYRNLVRAGESAGV
LDTVLDTVATYKERMEGIKAKIKKALFYPVMVLAVALLVSMIMLLFVVPVFAQTFRDAGAELPAPTQIVINASEFMKAYW
WLVIGSIVGGVVALVMGKRRSVKFAHFLDRLMLKLPVIGNILRQSAIARFARTLGVTFKAGVPLVEALDAVAGATGSVVY
SDAVKAMRDDIAVGHQLQLAMRQTGLFPNMVVQMTAIGEESGALDSMLFKVAEFYEEEVNNAVDTLSTLLEPLIMVILGV
LVGGMVISLYLPIFKLAATV

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=174609 ATSB10_RS10355 WP_063672587.1 2260735..2261997(+) (pilC) [Dyella thiooxydans strain ATSB10]
ATGGCTACGGCGACGGCAAAGAATCCGCGGGATCTGGGCGCCCAGCGGGCCCAGGTCAGTCAGGCGACCGTGTACGACTG
GGTGGCCCTGGACAAGCGCGGCAAGCGCATGAAGGGCGAGATGCCCGCCAAGAACGCCTCGCTGGTCAAGGCGGAACTGC
GCCGCCAGGGCATGAACCCGCAGACGGTGAAGGAACGGGGCAAGCCGCTGTTCGGCGCCACCGGCAGCACGGTCAAGCCG
CGCGACGTGGCCATCTTCAGCCGCCAGATCGCCACCATGATGGCTTCGGGCGTGCCGATGGTGCAGTCCTTCGAAATCAT
CGCCGACGGCCAGAAGAACGTCCGCTTCAAGAACATCCTGACGGACGTGAAGCAGGGTATCGAAGGCGGTGCCTCGCTGA
ACGAGGCCCTGGCCAAATACCCCATCCAGTTCGACGAGCTCTACCGCAACCTGGTCCGTGCCGGCGAATCGGCCGGTGTG
CTGGATACCGTGCTGGACACGGTGGCCACCTACAAGGAGCGGATGGAGGGCATCAAGGCCAAGATCAAGAAGGCCCTGTT
CTACCCGGTGATGGTGCTGGCCGTGGCGCTGCTGGTCAGCATGATCATGCTGCTGTTCGTGGTGCCGGTGTTTGCCCAGA
CGTTCCGCGACGCCGGAGCGGAGCTCCCCGCCCCCACCCAGATCGTGATCAACGCCTCGGAGTTCATGAAAGCGTACTGG
TGGCTGGTGATCGGCAGCATCGTCGGCGGCGTCGTGGCGCTGGTGATGGGCAAGAGGCGTTCGGTCAAGTTTGCCCACTT
CCTCGACCGCCTGATGCTGAAGCTGCCGGTGATCGGCAACATCCTGCGCCAGTCGGCCATCGCCCGCTTCGCCCGCACGC
TGGGTGTGACCTTCAAGGCGGGCGTACCGCTGGTGGAAGCACTCGATGCGGTGGCCGGCGCGACCGGCAGCGTGGTCTAC
AGCGACGCCGTGAAGGCCATGCGTGATGACATCGCCGTCGGCCACCAGCTGCAGCTGGCGATGCGCCAGACCGGCCTGTT
TCCCAACATGGTGGTGCAGATGACTGCCATCGGCGAGGAGTCCGGTGCCCTGGACAGCATGCTGTTCAAGGTGGCCGAGT
TCTACGAGGAAGAGGTCAACAACGCCGTCGATACCCTTTCCACCCTGCTCGAGCCGCTGATCATGGTGATCCTGGGTGTG
CTGGTGGGCGGCATGGTGATCTCGCTCTACCTGCCGATCTTCAAGCTGGCGGCAACCGTCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(86-209)

Type II secretion system (T2SS), protein F (T2SSF)

(290-412)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A160N2F2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

54.04

94.286

0.51
  pilC Pseudomonas stutzeri DSM 10701

53.266

94.762

0.505
  pilC Acinetobacter baumannii D1279779

49.268

97.619

0.481
  pilC Acinetobacter baylyi ADP1

48.049

97.619

0.469
  pilC Thermus thermophilus HB27

38.995

99.524

0.388
  pilC Vibrio campbellii strain DS40M4

40.302

94.524

0.381
  pilC Vibrio cholerae strain A1552

40.816

93.333

0.381
  pilG Neisseria gonorrhoeae MS11

40.933

91.905

0.376
  pilG Neisseria meningitidis 44/76-A

40.933

91.905

0.376

Multiple sequence alignment