Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ATSB10_RS10350 Genome accession   NZ_CP014841
Coordinates   2258874..2260601 (+) Length   575 a.a.
NCBI ID   WP_063672585.1    Uniprot ID   A0A160N222
Organism   Dyella thiooxydans strain ATSB10     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2253874..2265601
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ATSB10_RS10330 (ATSB10_21180) - 2255025..2255579 (+) 555 WP_236886400.1 class I SAM-dependent methyltransferase -
  ATSB10_RS10335 - 2255576..2256613 (-) 1038 WP_063672579.1 glycosyltransferase -
  ATSB10_RS10340 (ATSB10_21200) - 2256610..2257776 (-) 1167 WP_063672581.1 glycosyltransferase family 4 protein -
  ATSB10_RS10345 (ATSB10_21210) - 2257825..2258712 (-) 888 WP_063672583.1 glycosyltransferase family 2 protein -
  ATSB10_RS10350 (ATSB10_21230) pilB 2258874..2260601 (+) 1728 WP_063672585.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ATSB10_RS10355 (ATSB10_21240) pilC 2260735..2261997 (+) 1263 WP_063672587.1 type II secretion system F family protein Machinery gene
  ATSB10_RS10360 (ATSB10_21250) - 2262071..2262931 (+) 861 WP_063672589.1 A24 family peptidase -
  ATSB10_RS10365 (ATSB10_21260) coaE 2262939..2263544 (+) 606 WP_063672591.1 dephospho-CoA kinase -
  ATSB10_RS10370 (ATSB10_21270) - 2263549..2263947 (-) 399 WP_063672593.1 NUDIX domain-containing protein -
  ATSB10_RS10375 (ATSB10_21280) - 2263947..2264435 (-) 489 WP_063672595.1 dihydrofolate reductase -
  ATSB10_RS10380 (ATSB10_21290) - 2264574..2265368 (-) 795 WP_063672597.1 thymidylate synthase -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62647.15 Da        Isoelectric Point: 6.2662

>NTDB_id=174608 ATSB10_RS10350 WP_063672585.1 2258874..2260601(+) (pilB) [Dyella thiooxydans strain ATSB10]
MSSQVQASLAGLSGMARRLVSEGVVPEADVRKAVADSTQAKATLATWLLDHQLVDSTRFTQVASVEFGMPMMDVTSLAPA
SMPLNLISEALVTKHRALPLFRRGKRLYVGIADPMQSHALDEIKFHSNCMVEPILVERGQLQRAIDGAMSAMNANISDMG
GSDLDDLELETSGGDEEADQTGIDATGGDDAPVVKFVNKVLVDAIRRGASDIHFEPFESQYRVRFRMDGMLRAVASPPMK
LASRISSRLKVMAQLDIAEKRVPQDGRIKLNLSKSRAVDFRVSTLPTLFGEKIVLRILDGSAAKIGIEKLGYEPEQQKLY
VDAIHKPYGMVLVTGPTGSGKTVSLYTALNMLNTAERNISTVEDPVEIRVEGINQVQQNTKRGMTFAAALRSFLRQDPDV
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDASQTISRLMNMGIAPYNITSSVTLIVAQRLARRLHDCKKPIELPPET
LLAAGFTQKDIDEGMTIYEAVGCDGCNEGYKGRVGIYQVMPMLEEIQKIILKGGNALQIAETAKEAGINDLRASALLKVK
NGLTSLTEIDRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=174608 ATSB10_RS10350 WP_063672585.1 2258874..2260601(+) (pilB) [Dyella thiooxydans strain ATSB10]
ATGTCATCTCAAGTGCAGGCATCCCTTGCCGGTCTCTCGGGCATGGCCCGCAGGCTGGTCTCGGAGGGCGTGGTGCCGGA
GGCTGATGTCCGCAAGGCCGTGGCAGATTCGACCCAGGCCAAGGCCACGCTGGCCACCTGGCTGCTCGACCACCAGCTGG
TCGACAGCACGCGCTTCACCCAGGTGGCGTCGGTCGAGTTCGGCATGCCGATGATGGACGTGACCTCCCTTGCTCCGGCC
AGCATGCCGCTGAACCTGATCAGCGAGGCGCTGGTCACCAAGCATCGCGCCCTGCCGCTGTTCCGTCGCGGCAAGCGCCT
GTACGTCGGCATCGCCGACCCGATGCAGTCGCACGCGCTGGACGAGATCAAGTTCCACTCCAACTGCATGGTGGAGCCGA
TCCTGGTCGAACGCGGGCAGTTGCAGCGGGCGATCGACGGCGCGATGAGCGCCATGAACGCCAACATCTCCGACATGGGC
GGCAGCGATCTCGACGACCTCGAGCTCGAGACTTCCGGTGGCGACGAGGAGGCCGACCAGACAGGCATCGACGCGACCGG
GGGCGATGACGCCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTCGACGCGATCCGCCGGGGCGCCTCGGACATCCACT
TCGAGCCATTCGAGAGCCAGTACCGCGTCCGCTTCCGCATGGACGGCATGCTGCGCGCCGTGGCCAGCCCGCCGATGAAG
CTGGCCAGCCGGATCTCCTCGCGCCTGAAGGTGATGGCCCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGACGGGCG
CATCAAGCTCAACCTGTCCAAGAGTCGCGCGGTCGACTTCCGTGTCAGCACCCTGCCCACGCTGTTCGGCGAGAAGATCG
TGCTGCGTATCCTGGACGGCTCGGCCGCCAAGATCGGTATCGAGAAGCTCGGCTACGAGCCGGAACAGCAGAAACTCTAC
GTGGACGCGATCCACAAGCCCTACGGCATGGTGCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTGTCGCTGTATAC
CGCGCTCAACATGCTCAACACCGCCGAGCGCAATATCTCCACCGTGGAAGACCCGGTCGAAATCCGCGTCGAGGGCATCA
ACCAGGTGCAGCAGAACACCAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGCTCCTTCCTGCGCCAGGATCCGGACGTG
ATCATGGTCGGCGAAATCCGCGACCTGGAAACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGTCACATGGTGCTGTC
GACACTGCACACCAACGACGCCTCCCAGACCATCTCGCGACTGATGAACATGGGCATCGCGCCCTACAACATCACCTCGT
CGGTCACCCTGATCGTGGCGCAGCGCCTGGCGCGACGCCTGCACGACTGCAAGAAGCCGATCGAGTTGCCGCCCGAGACC
TTGCTGGCTGCCGGTTTCACGCAAAAGGACATCGACGAGGGCATGACCATCTACGAAGCGGTCGGCTGCGATGGCTGCAA
CGAGGGCTACAAGGGGCGCGTGGGTATCTACCAGGTGATGCCGATGCTCGAGGAGATCCAGAAGATCATCCTCAAGGGCG
GCAATGCGCTGCAGATCGCTGAGACCGCCAAGGAGGCCGGCATCAACGACCTCCGCGCCTCGGCCCTGCTCAAGGTGAAG
AACGGCCTGACCAGCCTGACCGAAATCGACCGCGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-152)

Type II/IV secretion system protein (T2SSE)

(199-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A160N222

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.674

98.609

0.539
  pilB Acinetobacter baumannii D1279779

53.792

98.609

0.53
  pilB Legionella pneumophila strain ERS1305867

50.88

98.783

0.503
  pilF Neisseria gonorrhoeae MS11

47.527

98.435

0.468
  pilB Vibrio cholerae strain A1552

48.694

93.217

0.454
  pilB Vibrio parahaemolyticus RIMD 2210633

47.932

92.522

0.443
  pilB Vibrio campbellii strain DS40M4

47.826

92

0.44
  pilF Thermus thermophilus HB27

36.824

99.652

0.367
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.095

94.957

0.362

Multiple sequence alignment