Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   A0T30_RS18675 Genome accession   NZ_CP014784
Coordinates   4051459..4052676 (+) Length   405 a.a.
NCBI ID   WP_061905375.1    Uniprot ID   -
Organism   Aquipseudomonas alcaligenes strain NEB 585     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 4043987..4056249 4051459..4052676 within 0


Gene organization within MGE regions


Location: 4043987..4056249
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A0T30_RS18630 (A0T30_18630) - 4043987..4044235 (+) 249 WP_061905368.1 hypothetical protein -
  A0T30_RS21190 (A0T30_18635) - 4044228..4044617 (+) 390 WP_061905369.1 hypothetical protein -
  A0T30_RS18640 (A0T30_18640) - 4045003..4046283 (+) 1281 WP_061905370.1 hypothetical protein -
  A0T30_RS18645 (A0T30_18645) - 4046280..4047272 (+) 993 WP_061905745.1 tyrosine-type recombinase/integrase -
  A0T30_RS21040 - 4047331..4047810 (+) 480 WP_156480592.1 hypothetical protein -
  A0T30_RS18660 (A0T30_18660) tfpZ 4048040..4048789 (-) 750 WP_061905372.1 TfpX/TfpZ family type IV pilin accessory protein -
  A0T30_RS18665 (A0T30_18665) - 4049081..4049557 (+) 477 WP_061905373.1 pilin -
  A0T30_RS18670 (A0T30_18670) pilB 4049749..4051452 (+) 1704 WP_061905374.1 type IV-A pilus assembly ATPase PilB Machinery gene
  A0T30_RS18675 (A0T30_18675) pilC 4051459..4052676 (+) 1218 WP_061905375.1 type II secretion system F family protein Machinery gene
  A0T30_RS18680 (A0T30_18680) pilD 4052680..4053552 (+) 873 WP_156480593.1 A24 family peptidase Machinery gene
  A0T30_RS18685 (A0T30_18685) coaE 4053549..4054160 (+) 612 WP_061905376.1 dephospho-CoA kinase -
  A0T30_RS18690 (A0T30_18690) yacG 4054157..4054351 (+) 195 WP_021702222.1 DNA gyrase inhibitor YacG -
  A0T30_RS18695 (A0T30_18695) - 4054355..4054570 (-) 216 WP_061905747.1 hypothetical protein -
  A0T30_RS18700 (A0T30_18700) - 4054661..4055356 (-) 696 WP_061905377.1 energy-coupling factor ABC transporter permease -
  A0T30_RS18705 (A0T30_18705) - 4055353..4055829 (-) 477 WP_061905378.1 hypothetical protein -
  A0T30_RS18710 (A0T30_18710) - 4055833..4056249 (-) 417 WP_061905379.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44233.98 Da        Isoelectric Point: 9.6717

>NTDB_id=174036 A0T30_RS18675 WP_061905375.1 4051459..4052676(+) (pilC) [Aquipseudomonas alcaligenes strain NEB 585]
MAEKALKTSTFSWEGTDRKGAKVKGEMGGQSPALVKAQLRKQGINPIKVRKKSVSLFSAGKSIKPMDIALFTRQMATMMK
AGVPLLSSFDIIGEGFDNPNMRKMVDELKQEVAAGNSFASSLRKKPIQFDDLYCNLVEAGEQAGALETLLDRVATYKEKT
EALKAKIKKAMTYPIAVLVVAVIVSAILLIKVVPQFEDVFKNFGAELPAFTQMVIGISQALQEWWFVVLIGLFVVAFGVR
EIHRRSENFRNKVDQLTLKVPIVGDIIYKSSVARYARTLSTTFAAGVPLVEALDSVAGATGNVVFRNAVHKIKQDVSTGM
QLNFSMRTTGVFPSMALQMTAIGEESGALDDMLDKVASFYETEVDNMVDNLTALMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGQVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=174036 A0T30_RS18675 WP_061905375.1 4051459..4052676(+) (pilC) [Aquipseudomonas alcaligenes strain NEB 585]
ATGGCGGAAAAAGCTCTCAAAACCAGCACTTTCAGCTGGGAAGGCACAGACAGAAAAGGCGCCAAAGTCAAAGGTGAGAT
GGGCGGACAAAGCCCGGCACTGGTAAAGGCCCAGCTGCGCAAGCAGGGCATCAACCCGATCAAGGTCCGCAAGAAGTCCG
TCTCGCTATTCAGCGCCGGCAAATCGATCAAGCCGATGGACATTGCGCTGTTCACCCGGCAGATGGCGACCATGATGAAG
GCGGGCGTTCCGCTGCTCAGCTCCTTCGACATTATTGGGGAAGGCTTTGACAACCCCAACATGCGCAAGATGGTGGACGA
GCTCAAGCAGGAGGTGGCTGCGGGTAACAGCTTTGCCTCCTCTTTGCGCAAGAAGCCGATCCAGTTCGATGATCTCTATT
GCAACCTGGTCGAAGCAGGTGAGCAGGCCGGTGCCCTGGAAACCCTGCTGGATCGGGTCGCCACCTATAAAGAGAAAACC
GAAGCACTGAAAGCCAAGATCAAGAAGGCCATGACTTACCCAATTGCAGTACTGGTGGTGGCGGTCATCGTCTCGGCCAT
TCTGCTGATCAAGGTCGTACCCCAGTTCGAAGATGTCTTCAAAAACTTTGGTGCAGAGCTACCAGCCTTTACTCAGATGG
TCATCGGCATCTCGCAGGCTCTGCAGGAGTGGTGGTTCGTCGTACTGATTGGACTATTCGTCGTGGCTTTCGGAGTTCGT
GAAATCCATCGACGCTCCGAGAACTTCCGCAACAAAGTCGATCAGCTGACTCTCAAAGTGCCGATTGTCGGCGACATCAT
CTACAAGTCGTCCGTCGCCCGTTATGCACGCACCCTGTCCACCACCTTCGCCGCCGGCGTGCCACTGGTCGAAGCACTGG
ACTCGGTAGCCGGGGCCACCGGCAACGTGGTATTCCGCAACGCCGTGCACAAGATCAAGCAGGATGTATCGACCGGCATG
CAGCTGAACTTCTCCATGCGCACCACCGGCGTATTCCCCAGCATGGCGCTGCAAATGACCGCCATTGGCGAGGAGTCGGG
CGCTCTCGACGACATGTTGGACAAGGTGGCCAGTTTCTACGAAACCGAAGTCGACAACATGGTGGACAACCTCACCGCGC
TGATGGAGCCCATGATCATGGCGGTGCTGGGCGTCCTGGTGGGCGGCTTGATCATCGCCATGTACCTGCCCATTTTCCAG
CTCGGCCAAGTCGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

82.963

100

0.83
  pilC Acinetobacter baumannii D1279779

59.012

100

0.59
  pilC Acinetobacter baylyi ADP1

58.374

100

0.585
  pilC Legionella pneumophila strain ERS1305867

51.385

98.025

0.504
  pilG Neisseria gonorrhoeae MS11

44.279

99.259

0.44
  pilG Neisseria meningitidis 44/76-A

44.03

99.259

0.437
  pilC Vibrio cholerae strain A1552

42.469

100

0.425
  pilC Vibrio campbellii strain DS40M4

40.704

98.272

0.4
  pilC Thermus thermophilus HB27

36.75

98.765

0.363

Multiple sequence alignment