Detailed information NTG0174035

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: A0T30_RS18670
• Genome accession: NZ_CP014784
• Coordinates: 4049749..4051452 (+)
• Length: 567 a.a.
• NCBI ID: WP_061905374.1
• Uniprot ID: -
• Organism: Aquipseudomonas alcaligenes strain NEB 585
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	4043987..4056249	4049749..4051452	within	0

Gene organization within MGE regions

Location: 4043987..4056249

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A0T30_RS18630 (A0T30_18630)	-	4043987..4044235 (+)	249	WP_061905368.1	hypothetical protein	-
A0T30_RS21190 (A0T30_18635)	-	4044228..4044617 (+)	390	WP_061905369.1	hypothetical protein	-
A0T30_RS18640 (A0T30_18640)	-	4045003..4046283 (+)	1281	WP_061905370.1	hypothetical protein	-
A0T30_RS18645 (A0T30_18645)	-	4046280..4047272 (+)	993	WP_061905745.1	tyrosine-type recombinase/integrase	-
A0T30_RS21040	-	4047331..4047810 (+)	480	WP_156480592.1	hypothetical protein	-
A0T30_RS18660 (A0T30_18660)	tfpZ	4048040..4048789 (-)	750	WP_061905372.1	TfpX/TfpZ family type IV pilin accessory protein	-
A0T30_RS18665 (A0T30_18665)	-	4049081..4049557 (+)	477	WP_061905373.1	pilin	-
A0T30_RS18670 (A0T30_18670)	pilB	4049749..4051452 (+)	1704	WP_061905374.1	type IV-A pilus assembly ATPase PilB	Machinery gene
A0T30_RS18675 (A0T30_18675)	pilC	4051459..4052676 (+)	1218	WP_061905375.1	type II secretion system F family protein	Machinery gene
A0T30_RS18680 (A0T30_18680)	pilD	4052680..4053552 (+)	873	WP_156480593.1	A24 family peptidase	Machinery gene
A0T30_RS18685 (A0T30_18685)	coaE	4053549..4054160 (+)	612	WP_061905376.1	dephospho-CoA kinase	-
A0T30_RS18690 (A0T30_18690)	yacG	4054157..4054351 (+)	195	WP_021702222.1	DNA gyrase inhibitor YacG	-
A0T30_RS18695 (A0T30_18695)	-	4054355..4054570 (-)	216	WP_061905747.1	hypothetical protein	-
A0T30_RS18700 (A0T30_18700)	-	4054661..4055356 (-)	696	WP_061905377.1	energy-coupling factor ABC transporter permease	-
A0T30_RS18705 (A0T30_18705)	-	4055353..4055829 (-)	477	WP_061905378.1	hypothetical protein	-
A0T30_RS18710 (A0T30_18710)	-	4055833..4056249 (-)	417	WP_061905379.1	GNAT family N-acetyltransferase	-

Sequence

Protein

• Length: 567 a.a.
• Molecular weight: 62445.47 Da
• Isoelectric Point: 5.3337

>NTDB_id=174035 A0T30_RS18670 WP_061905374.1 4049749..4051452(+) (pilB) [Aquipseudomonas alcaligenes strain NEB 585]
MNELSPLTGLAKQLVMNNLLDETSAQQAQLQAQRNKLPLVTYLAQNKLVKSRVLAELISDQFGIAMLDLGSIDKESMPRD
TVTEKLARQHRVLPLFKRGNKLFIATSDPSNHQAVTDVQFSTGLSTEALLVEDDKLGDAIEKFFDNASAGLADLGDVDLD
NVDIESVDDDKQDSGSGGDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYERAYRVRFRTDGILHEVARPPIQLAPRISAR
LKVMASLDISERRKPQDGRIKMKISKTKAIDFRVNTLPTLWGEKIVMRILDPTSAQMGIDALGYEDDQKELYMNALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPKQGMDFSQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCSTCKKPVDVPHEVLLKEGFP
EDKIGTFKIYGPVGCENCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARRDGFNDLRMSGLQKAMQGVTSLEE
INRVTKD

Nucleotide

• Length: 1704 bp

>NTDB_id=174035 A0T30_RS18670 WP_061905374.1 4049749..4051452(+) (pilB) [Aquipseudomonas alcaligenes strain NEB 585]
ATGAACGAACTCTCCCCCCTGACCGGCCTGGCCAAGCAACTGGTCATGAACAACCTCCTGGACGAGACCTCGGCTCAGCA
GGCTCAGCTCCAGGCGCAACGCAACAAGCTCCCCCTCGTCACCTACCTGGCCCAGAACAAGCTGGTGAAGAGCCGTGTAC
TGGCAGAGCTGATCAGCGACCAGTTCGGCATCGCCATGCTCGACCTCGGCAGCATCGACAAAGAGTCCATGCCGCGCGAT
ACGGTGACCGAGAAACTGGCACGACAGCATCGCGTACTGCCGCTGTTCAAGCGCGGCAACAAGCTGTTCATTGCCACCTC
GGATCCCTCCAACCACCAGGCCGTAACCGATGTGCAGTTCAGCACCGGGCTTAGCACCGAAGCCCTGCTGGTCGAGGATG
ACAAGCTCGGCGATGCCATCGAGAAGTTCTTCGACAACGCCTCGGCTGGCCTCGCAGACCTTGGCGATGTCGACCTGGAC
AATGTCGATATCGAGAGCGTGGATGACGACAAACAGGACTCCGGCAGCGGCGGCGACGCCGACGATGCGCCGGTAGTGCG
CTTCGTCAACAAGATGCTGCTGGACGCCATCAAGGGTGGCTCATCCGACTTGCACTTCGAACCTTACGAGCGCGCCTACC
GTGTGCGCTTCCGCACCGACGGCATCCTGCATGAAGTAGCCAGGCCGCCGATACAGCTGGCCCCGCGCATCTCTGCACGC
CTGAAGGTGATGGCCAGCCTGGATATCTCCGAGCGGCGCAAGCCGCAGGATGGCCGGATCAAGATGAAGATCTCCAAAAC
CAAGGCCATCGACTTCCGCGTCAACACCCTACCCACCCTGTGGGGCGAGAAGATCGTGATGCGAATCCTCGACCCCACCA
GCGCGCAGATGGGCATCGACGCCCTCGGCTACGAGGATGATCAGAAAGAACTGTATATGAACGCCCTGAAACAGCCCCAG
GGCATGATTCTGGTCACCGGCCCCACCGGGTCCGGCAAGACGGTCTCCCTTTATACCGGTCTGAACATCCTCAATACGGT
GGACGTGAACATCTCCACCGCCGAAGACCCCGTGGAGATCAACCTGGAAGGTATCAACCAGGTCAACGTCAATCCCAAGC
AGGGCATGGACTTCTCCCAGGCCCTGCGCGCCTTCCTGCGCCAGGACCCGGACATCATCATGGTGGGTGAGATCCGCGAC
CTGGAAACCGCCGAAATCGCCATCAAGGCCGCGCAGACCGGCCACATGGTGATGTCCACCCTGCACACCAACAGCGCGGC
CGAAACCCTGACGCGCCTGCGCAACATGGGCGTACCGGCCTTCAACATCGCCACCTCGGTCAACCTGATCATTGCCCAGC
GCCTCGCGCGCAAGCTCTGCAGCACCTGCAAGAAGCCGGTAGACGTGCCCCATGAAGTGCTGCTGAAAGAAGGCTTCCCG
GAAGACAAGATCGGCACCTTCAAGATCTACGGGCCGGTCGGCTGCGAGAACTGCAAGGGTGGCTACAAGGGCCGGGTTGG
TATTTATGAAGTGGTTAAAAACACTCCCGCCCTGCAACGGATTATCATGGAGGAAGGTAACTCCATTGAAATCGCCGAGC
AGGCTCGCCGGGATGGTTTCAATGATCTGCGCATGTCCGGCCTGCAAAAGGCCATGCAAGGCGTAACCAGCCTTGAGGAA
ATCAACCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (191-458)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-148)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baylyi ADP1	60.106	99.471	0.598
pilB	Acinetobacter baumannii D1279779	59.752	99.471	0.594
pilB	Legionella pneumophila strain ERS1305867	55.575	99.647	0.554
pilF	Neisseria gonorrhoeae MS11	49.293	99.824	0.492
pilB	Vibrio cholerae strain A1552	49.821	98.765	0.492
pilB	Vibrio campbellii strain DS40M4	48.246	100	0.485
pilB	Vibrio parahaemolyticus RIMD 2210633	50.564	93.827	0.474
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.434	93.474	0.369
pilB/pilB1	Synechocystis sp. PCC 6803	38.306	95.767	0.367