Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   AYR47_RS11275 Genome accession   NZ_CP014546
Coordinates   2432366..2433574 (-) Length   402 a.a.
NCBI ID   WP_061435288.1    Uniprot ID   A0A127HWF7
Organism   Pseudomonas azotoformans strain S4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2427366..2438574
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AYR47_RS11245 (AYR47_11245) - 2427611..2429665 (+) 2055 WP_082781488.1 autotransporter outer membrane beta-barrel domain-containing protein -
  AYR47_RS11250 (AYR47_11250) - 2429718..2430407 (+) 690 WP_061435285.1 energy-coupling factor ABC transporter permease -
  AYR47_RS11255 (AYR47_11255) - 2430474..2430689 (+) 216 WP_003171680.1 hypothetical protein -
  AYR47_RS11260 (AYR47_11260) yacG 2430686..2430886 (-) 201 WP_003188437.1 DNA gyrase inhibitor YacG -
  AYR47_RS11265 (AYR47_11265) coaE 2430883..2431506 (-) 624 WP_033903342.1 dephospho-CoA kinase -
  AYR47_RS11270 (AYR47_11270) pilD 2431503..2432369 (-) 867 WP_061435287.1 prepilin peptidase Machinery gene
  AYR47_RS11275 (AYR47_11275) pilC 2432366..2433574 (-) 1209 WP_061435288.1 type II secretion system F family protein Machinery gene
  AYR47_RS11280 (AYR47_11280) - 2433769..2434191 (+) 423 WP_082462009.1 pilin -
  AYR47_RS11285 (AYR47_11285) - 2434227..2435627 (+) 1401 WP_061435290.1 O-antigen ligase family protein -
  AYR47_RS11295 (AYR47_11295) - 2435894..2436199 (+) 306 WP_033903347.1 DUF6388 family protein -
  AYR47_RS11300 (AYR47_11300) nadC 2436288..2437137 (-) 850 Protein_2274 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43195.57 Da        Isoelectric Point: 9.8437

>NTDB_id=172480 AYR47_RS11275 WP_061435288.1 2432366..2433574(-) (pilC) [Pseudomonas azotoformans strain S4]
MDDALTLYAWEGINRKGRRVCGQTTGHNLAWVRAQLRQQGISPGHVRKKSPVLRGFTPSIKSTDITLFTRQLATLLKAGI
PLLQAFAVIGDGFDNRAVRELVQGLKQAIAAGSSLAEALRKQPRCFDELYCNLVAAGEQAGALETLLERVAIHREKSEQL
RARIKKAMTYPIAVLVVASLVTGVLLIHVVPQFQNLFAGVDGTLPGFTLQVIALSEFVQKAWWIVALGILAGGMGLRHAY
NAAPAFRLWLDAGLLKAPLAGKLLTKSAVARYARTLSTTFAAGVPLVQALDSVAGAVGSGPFKQAIEHMRHDVSTGLPLN
QSMLASGLFPPMAIQMTAIGEESGTLDRMLEKVANHYEADVDNLVDNLTSLMEPLIMVVLGSIVGALVIAMYLPVFQLGS
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=172480 AYR47_RS11275 WP_061435288.1 2432366..2433574(-) (pilC) [Pseudomonas azotoformans strain S4]
ATGGACGACGCTTTAACGCTGTACGCCTGGGAAGGCATCAATCGCAAAGGACGCAGGGTGTGCGGGCAAACCACGGGGCA
CAATCTTGCCTGGGTCAGGGCCCAGTTGCGCCAACAGGGCATCAGCCCAGGGCATGTCCGCAAGAAATCCCCCGTTCTAC
GAGGATTCACACCGTCGATCAAATCGACGGATATCACCCTGTTCACTCGCCAACTGGCGACGCTGCTAAAGGCCGGGATT
CCCCTGCTGCAAGCTTTCGCCGTCATCGGCGATGGCTTCGACAACCGCGCTGTTCGCGAGTTGGTGCAGGGGCTGAAACA
AGCGATCGCCGCAGGTTCCAGCCTGGCCGAAGCCTTGCGCAAACAACCGCGCTGCTTCGATGAGCTGTATTGCAATCTGG
TGGCGGCCGGCGAGCAGGCCGGAGCGCTGGAAACCCTGCTGGAGCGTGTGGCGATCCATCGGGAAAAGAGCGAGCAATTA
AGGGCCAGGATCAAAAAAGCCATGACCTATCCGATCGCGGTGCTGGTGGTGGCCAGCCTGGTCACCGGTGTTCTGCTGAT
CCACGTCGTGCCGCAGTTCCAGAACCTGTTTGCCGGCGTCGACGGCACGCTACCCGGTTTCACCTTGCAGGTGATCGCCT
TGTCCGAGTTTGTGCAGAAAGCCTGGTGGATCGTAGCGCTGGGCATTCTCGCGGGAGGCATGGGGTTGCGCCACGCCTAT
AACGCCGCCCCTGCTTTTCGACTCTGGCTGGATGCCGGTTTGTTGAAAGCGCCGCTGGCAGGCAAACTGCTGACAAAATC
GGCCGTGGCCCGCTACGCCCGCACACTGTCCACGACCTTTGCGGCGGGTGTCCCATTGGTTCAGGCATTGGATTCAGTAG
CCGGCGCGGTCGGTTCAGGGCCGTTCAAACAGGCAATCGAACATATGCGACACGATGTATCCACCGGCCTGCCATTGAAT
CAATCCATGCTCGCCAGCGGCCTGTTCCCTCCCATGGCGATCCAGATGACTGCCATCGGCGAAGAGTCGGGCACACTGGA
CCGTATGCTGGAAAAGGTCGCCAATCATTACGAGGCCGATGTGGACAACCTGGTCGATAACCTCACCAGCCTGATGGAGC
CGCTGATCATGGTGGTCCTGGGCAGCATCGTCGGCGCGCTGGTCATCGCCATGTACTTGCCGGTCTTCCAGCTGGGGAGC
GCGTTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(273-394)

Type II secretion system (T2SS), protein F (T2SSF)

(68-191)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A127HWF7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

57.975

98.259

0.57
  pilC Acinetobacter baumannii D1279779

51.139

98.259

0.502
  pilC Acinetobacter baylyi ADP1

50

99.005

0.495
  pilC Legionella pneumophila strain ERS1305867

48.622

99.254

0.483
  pilC Vibrio cholerae strain A1552

41.667

98.507

0.41
  pilG Neisseria meningitidis 44/76-A

40.885

95.522

0.391
  pilG Neisseria gonorrhoeae MS11

41.099

95.025

0.391
  pilC Vibrio campbellii strain DS40M4

38.579

98.01

0.378
  pilC Thermus thermophilus HB27

37.037

100

0.373

Multiple sequence alignment