Detailed information NTG0172479

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AYR47_RS11270
• Genome accession: NZ_CP014546
• Coordinates: 2431503..2432369 (-)
• Length: 288 a.a.
• NCBI ID: WP_061435287.1
• Uniprot ID: A0A127HWE2
• Organism: Pseudomonas azotoformans strain S4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2426503..2437369

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AYR47_RS11240 (AYR47_11240)	-	2426728..2427357 (-)	630	WP_033903339.1	DUF1780 domain-containing protein	-
AYR47_RS11245 (AYR47_11245)	-	2427611..2429665 (+)	2055	WP_082781488.1	autotransporter outer membrane beta-barrel domain-containing protein	-
AYR47_RS11250 (AYR47_11250)	-	2429718..2430407 (+)	690	WP_061435285.1	energy-coupling factor ABC transporter permease	-
AYR47_RS11255 (AYR47_11255)	-	2430474..2430689 (+)	216	WP_003171680.1	hypothetical protein	-
AYR47_RS11260 (AYR47_11260)	yacG	2430686..2430886 (-)	201	WP_003188437.1	DNA gyrase inhibitor YacG	-
AYR47_RS11265 (AYR47_11265)	coaE	2430883..2431506 (-)	624	WP_033903342.1	dephospho-CoA kinase	-
AYR47_RS11270 (AYR47_11270)	pilD	2431503..2432369 (-)	867	WP_061435287.1	prepilin peptidase	Machinery gene
AYR47_RS11275 (AYR47_11275)	pilC	2432366..2433574 (-)	1209	WP_061435288.1	type II secretion system F family protein	Machinery gene
AYR47_RS11280 (AYR47_11280)	-	2433769..2434191 (+)	423	WP_082462009.1	pilin	-
AYR47_RS11285 (AYR47_11285)	-	2434227..2435627 (+)	1401	WP_061435290.1	O-antigen ligase family protein	-
AYR47_RS11295 (AYR47_11295)	-	2435894..2436199 (+)	306	WP_033903347.1	DUF6388 family protein	-
AYR47_RS11300 (AYR47_11300)	nadC	2436288..2437137 (-)	850	Protein_2274	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 31651.81 Da
• Isoelectric Point: 8.0050

>NTDB_id=172479 AYR47_RS11270 WP_061435287.1 2431503..2432369(-) (pilD) [Pseudomonas azotoformans strain S4]
MSLLLVEHPGAFVVMAGILGLIVGSFLNVLVWRLPKMLEREWRAQAHEVLGLPADPAGPTYNLMRPNSCCPHCNHPIRPW
ENIPLLSYLFLRGRCAHCKGAISPRYPLTELACGLISAWVAWHFGFGWQAGAVLVLSWGLLAMSLIDADHQLLPDVLVMP
LLWLGLIVNGFGLLTTLADALWGAVIGYMSLWSVFWLFKLVTGKDGMGHGDFKLLALLGAWGGWQILPMTLLMSSLVGVF
VGLILMRLHKAQASAPMPFGPYLAIAGWIALLWGGQITDFYLQSVGFR

Nucleotide

• Length: 867 bp

>NTDB_id=172479 AYR47_RS11270 WP_061435287.1 2431503..2432369(-) (pilD) [Pseudomonas azotoformans strain S4]
TTGAGCCTGTTACTGGTTGAACACCCGGGGGCCTTTGTGGTGATGGCAGGGATACTGGGGCTGATCGTCGGCAGTTTTCT
CAACGTGCTGGTGTGGCGCCTGCCAAAAATGCTCGAACGGGAATGGCGCGCCCAAGCCCATGAAGTGCTCGGTCTACCCG
CCGACCCAGCCGGGCCGACCTATAACCTGATGCGCCCCAATTCCTGCTGCCCGCATTGCAACCATCCGATACGCCCATGG
GAAAATATCCCACTGCTCAGTTACTTGTTTCTGCGAGGTCGTTGCGCCCATTGCAAAGGAGCAATCAGCCCTCGCTATCC
GCTTACTGAACTGGCGTGTGGACTGATCTCGGCCTGGGTGGCCTGGCATTTCGGCTTTGGCTGGCAGGCGGGCGCAGTGC
TTGTACTGAGCTGGGGCTTGCTGGCGATGAGCCTGATTGATGCCGATCATCAATTGTTGCCGGATGTGCTGGTGATGCCG
TTGCTGTGGCTGGGGCTGATCGTCAACGGGTTCGGTCTGCTGACGACATTGGCCGACGCACTGTGGGGCGCAGTGATCGG
GTATATGAGCCTGTGGAGCGTGTTCTGGCTGTTCAAGCTGGTCACTGGCAAGGACGGCATGGGCCACGGCGACTTCAAGT
TACTCGCGCTGCTCGGTGCCTGGGGCGGTTGGCAGATTCTGCCGATGACGCTGTTGATGTCGTCGTTGGTCGGTGTATTT
GTCGGCTTGATCCTGATGCGCCTGCACAAGGCCCAAGCGTCGGCGCCGATGCCATTTGGTCCGTATCTGGCCATTGCCGG
CTGGATTGCATTGCTCTGGGGTGGTCAAATAACCGACTTCTATTTGCAGTCTGTCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (18-124)

• Type IV leader peptidase family (Peptidase_A24) (134-243)

3D structure

• AlphaFold DB: A0A127HWE2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.48	96.875	0.528
pilD	Vibrio campbellii strain DS40M4	50.357	97.222	0.49
pilD	Neisseria gonorrhoeae MS11	51.111	93.75	0.479
pilD	Acinetobacter baumannii D1279779	46.429	97.222	0.451
pilD	Acinetobacter nosocomialis M2	46.429	97.222	0.451