Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   AZF00_RS14495 Genome accession   NZ_CP014544
Coordinates   3279153..3280364 (+) Length   403 a.a.
NCBI ID   WP_062384108.1    Uniprot ID   A0A127M8G0
Organism   Zhongshania aliphaticivorans strain SM-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3274153..3285364
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AZF00_RS14475 (AZF00_14475) nadC 3275686..3276525 (+) 840 WP_062384097.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AZF00_RS14480 (AZF00_14480) - 3276574..3277068 (-) 495 WP_062384100.1 pilin -
  AZF00_RS14490 (AZF00_14490) pilB 3277426..3279150 (+) 1725 WP_062384106.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AZF00_RS14495 (AZF00_14495) pilC 3279153..3280364 (+) 1212 WP_062384108.1 type II secretion system F family protein Machinery gene
  AZF00_RS14500 (AZF00_14500) pilD 3280401..3281285 (+) 885 WP_062384111.1 prepilin peptidase Machinery gene
  AZF00_RS14505 (AZF00_14505) coaE 3281312..3281920 (+) 609 WP_062384114.1 dephospho-CoA kinase -
  AZF00_RS14510 (AZF00_14510) - 3281954..3282157 (+) 204 WP_062384116.1 DNA gyrase inhibitor YacG -
  AZF00_RS14515 (AZF00_14515) mutT 3282185..3282607 (-) 423 WP_062384119.1 8-oxo-dGTP diphosphatase MutT -
  AZF00_RS14520 (AZF00_14520) argJ 3282588..3283808 (-) 1221 WP_062384121.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 403 a.a.        Molecular weight: 43632.23 Da        Isoelectric Point: 9.4851

>NTDB_id=172454 AZF00_RS14495 WP_062384108.1 3279153..3280364(+) (pilC) [Zhongshania aliphaticivorans strain SM-2]
MAAAKTETFVWQGVDKQGRKTRGELNAPSSAIVKAHLRKQGIAAKNVKKKTKPLFSNKKPIKPVDVAIFTRQMATMLKAG
VPLVQSFDIVAEGVENPTMQDLVTEIKNDVASGGGFAVACAKHPKYFDELFCSLIASGEASGTLEIMLDRVATYKEKTEA
LKAKIKKALTYPIAVLIVAAIVTSILLIKVVPQFASTFESFGSELPAFTQLVVAMSEWMQANWFIVLALTIISSFIFGKA
RQKYKRFSDWVDKMLLKAPIIGDIVYNSVIARFSRTLATTFGAGVPLVDALNSVAGAAGNAVYSKAIIQIRDDVTTGQTL
YSSIRFTNLFPSLLLQMVSIGEESGSLDDMLDKVATHYEEAVDNSVDALTSLLEPLIMSVLGVLVGGLMIAMYLPIFMLG
SAI

Nucleotide


Download         Length: 1212 bp        

>NTDB_id=172454 AZF00_RS14495 WP_062384108.1 3279153..3280364(+) (pilC) [Zhongshania aliphaticivorans strain SM-2]
ATGGCAGCAGCTAAAACCGAAACCTTTGTTTGGCAAGGTGTAGACAAGCAGGGTCGCAAAACTCGCGGCGAATTGAACGC
CCCGTCATCAGCTATTGTCAAAGCCCATTTGCGCAAACAAGGTATTGCTGCAAAAAATGTCAAGAAAAAGACAAAACCGC
TCTTTAGCAACAAAAAGCCCATCAAACCAGTTGACGTTGCCATTTTCACTCGACAAATGGCAACCATGCTTAAAGCCGGA
GTACCTCTTGTACAAAGCTTTGATATCGTTGCAGAAGGTGTAGAAAATCCCACCATGCAAGACCTCGTTACGGAAATCAA
AAATGACGTTGCTTCTGGCGGCGGATTTGCCGTAGCGTGCGCTAAACACCCAAAATACTTTGATGAACTTTTTTGTAGTT
TAATCGCATCAGGCGAAGCTTCAGGTACTTTAGAAATCATGCTAGATAGGGTTGCAACCTATAAAGAAAAGACTGAGGCA
CTAAAGGCAAAAATTAAGAAAGCACTCACCTACCCAATAGCTGTGCTAATAGTCGCCGCAATAGTCACTAGTATTCTTTT
AATAAAAGTCGTGCCTCAATTTGCATCTACATTTGAAAGTTTCGGATCAGAGCTGCCCGCATTCACTCAATTGGTTGTCG
CAATGTCCGAATGGATGCAAGCAAATTGGTTTATTGTTTTGGCACTTACAATCATTTCCTCATTTATCTTCGGAAAGGCT
AGACAAAAATACAAACGATTCTCCGACTGGGTTGACAAGATGTTACTCAAGGCGCCGATTATCGGCGACATCGTTTACAA
CTCAGTAATAGCAAGGTTTTCGCGCACCCTTGCAACGACCTTTGGTGCAGGGGTGCCCTTAGTAGACGCACTGAACTCTG
TCGCTGGCGCTGCTGGGAATGCTGTTTATTCCAAAGCAATTATTCAAATCCGAGACGATGTAACGACGGGACAAACTCTT
TACTCATCCATACGTTTCACAAATCTTTTTCCTAGCCTTTTATTACAAATGGTTTCAATTGGCGAAGAATCTGGCTCCCT
TGATGACATGCTCGACAAAGTAGCTACACACTATGAAGAAGCTGTAGACAACTCCGTTGATGCACTGACATCTCTTTTAG
AACCTCTAATCATGTCGGTTCTTGGAGTCTTAGTCGGCGGCCTAATGATCGCAATGTACCTCCCCATATTCATGCTTGGC
TCCGCCATTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(69-192)

Type II secretion system (T2SS), protein F (T2SSF)

(273-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A127M8G0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Acinetobacter baylyi ADP1

59.069

100

0.598
  pilC Pseudomonas stutzeri DSM 10701

60.1

99.504

0.598
  pilC Acinetobacter baumannii D1279779

58.291

98.759

0.576
  pilC Legionella pneumophila strain ERS1305867

55.639

99.007

0.551
  pilG Neisseria gonorrhoeae MS11

44.938

100

0.452
  pilG Neisseria meningitidis 44/76-A

44.938

100

0.452
  pilC Vibrio campbellii strain DS40M4

42.184

100

0.422
  pilC Vibrio cholerae strain A1552

41.032

100

0.414

Multiple sequence alignment