Detailed information NTG0172455

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AZF00_RS14500
• Genome accession: NZ_CP014544
• Coordinates: 3280401..3281285 (+)
• Length: 294 a.a.
• NCBI ID: WP_062384111.1
• Uniprot ID: A0A127M871
• Organism: Zhongshania aliphaticivorans strain SM-2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3275401..3286285

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AZF00_RS14475 (AZF00_14475)	nadC	3275686..3276525 (+)	840	WP_062384097.1	carboxylating nicotinate-nucleotide diphosphorylase	-
AZF00_RS14480 (AZF00_14480)	-	3276574..3277068 (-)	495	WP_062384100.1	pilin	-
AZF00_RS14490 (AZF00_14490)	pilB	3277426..3279150 (+)	1725	WP_062384106.1	type IV-A pilus assembly ATPase PilB	Machinery gene
AZF00_RS14495 (AZF00_14495)	pilC	3279153..3280364 (+)	1212	WP_062384108.1	type II secretion system F family protein	Machinery gene
AZF00_RS14500 (AZF00_14500)	pilD	3280401..3281285 (+)	885	WP_062384111.1	prepilin peptidase	Machinery gene
AZF00_RS14505 (AZF00_14505)	coaE	3281312..3281920 (+)	609	WP_062384114.1	dephospho-CoA kinase	-
AZF00_RS14510 (AZF00_14510)	-	3281954..3282157 (+)	204	WP_062384116.1	DNA gyrase inhibitor YacG	-
AZF00_RS14515 (AZF00_14515)	mutT	3282185..3282607 (-)	423	WP_062384119.1	8-oxo-dGTP diphosphatase MutT	-
AZF00_RS14520 (AZF00_14520)	argJ	3282588..3283808 (-)	1221	WP_062384121.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-

Sequence

Protein

• Length: 294 a.a.
• Molecular weight: 32490.60 Da
• Isoelectric Point: 4.9303

>NTDB_id=172455 AZF00_RS14500 WP_062384111.1 3280401..3281285(+) (pilD) [Zhongshania aliphaticivorans strain SM-2]
MSFFEAAANYPLLLITLLGFFGLLFGSFFNVVIYRLPKMMESTWKYECEILLSDDDAAAEEITPPQFNLAFPNSHCPKCN
APVKAWQNIPVVSYVLLRGRCASCKAPIGLRYPTVEVITALLTVFPLLMFGFTTYALAAILFSWILLILTVIDIDHQLLP
DQLTLPLIWLGLLFNSVTGSIPLQDAVWGAIGGYLSLWSVFWLFKLLTGKEGMGYGDFKLLAALGAWLGWQYLPLIILLS
SVVGAVFGGLVMLLRRSDSSMPMPFGPYLAGAGWIALFWGEALIDGYLQLAGIR

Nucleotide

• Length: 885 bp

>NTDB_id=172455 AZF00_RS14500 WP_062384111.1 3280401..3281285(+) (pilD) [Zhongshania aliphaticivorans strain SM-2]
ATGTCGTTCTTCGAAGCCGCCGCGAACTACCCGCTGCTTCTCATTACCTTATTAGGTTTCTTTGGCCTTCTCTTTGGCAG
CTTTTTCAATGTAGTGATCTACCGCTTACCCAAGATGATGGAATCTACGTGGAAATATGAGTGCGAAATTTTGCTCAGCG
ATGACGACGCAGCCGCTGAAGAAATCACGCCACCTCAGTTTAATTTGGCTTTTCCCAACTCCCACTGCCCAAAGTGTAAT
GCGCCGGTAAAGGCGTGGCAGAATATTCCTGTTGTTAGCTACGTGCTGCTGCGCGGGCGCTGCGCTTCTTGTAAGGCACC
GATCGGCCTGCGCTACCCTACGGTTGAGGTCATTACCGCGCTGCTTACCGTCTTCCCGCTGTTAATGTTTGGTTTCACCA
CCTATGCCCTTGCCGCCATTTTGTTTAGCTGGATCTTGCTGATTCTTACCGTTATTGATATTGACCACCAGTTGCTACCA
GACCAACTGACCTTACCCCTCATATGGCTGGGCCTTTTATTTAACAGCGTTACCGGCAGCATTCCATTACAGGATGCGGT
GTGGGGCGCCATTGGCGGCTATTTGTCGCTATGGTCCGTATTTTGGTTGTTTAAATTACTGACCGGTAAAGAGGGCATGG
GTTACGGCGATTTTAAATTACTCGCCGCACTGGGCGCTTGGCTGGGCTGGCAGTATTTGCCGCTAATTATTTTATTGTCT
TCTGTTGTCGGTGCCGTATTTGGCGGTTTAGTAATGCTGCTACGTCGCAGTGACAGTTCCATGCCCATGCCATTTGGCCC
CTATTTGGCCGGTGCTGGCTGGATCGCCCTGTTCTGGGGTGAAGCTCTGATTGACGGCTACCTTCAACTCGCTGGCATTC
GCTAG

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (141-248)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-128)

3D structure

• AlphaFold DB: A0A127M871

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.093	95.578	0.517
pilD	Vibrio campbellii strain DS40M4	51.049	97.279	0.497
pilD	Neisseria gonorrhoeae MS11	49.434	90.136	0.446
pilD	Acinetobacter baumannii D1279779	47.601	92.177	0.439
pilD	Acinetobacter nosocomialis M2	46.863	92.177	0.432