Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AZF00_RS14490 Genome accession   NZ_CP014544
Coordinates   3277426..3279150 (+) Length   574 a.a.
NCBI ID   WP_062384106.1    Uniprot ID   A0A127M869
Organism   Zhongshania aliphaticivorans strain SM-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3272426..3284150
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AZF00_RS14465 (AZF00_14465) ampD 3272484..3273032 (-) 549 WP_008251548.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AZF00_RS14470 (AZF00_14470) - 3273147..3275432 (-) 2286 WP_008251549.1 DUF1631 domain-containing protein -
  AZF00_RS14475 (AZF00_14475) nadC 3275686..3276525 (+) 840 WP_062384097.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AZF00_RS14480 (AZF00_14480) - 3276574..3277068 (-) 495 WP_062384100.1 pilin -
  AZF00_RS14490 (AZF00_14490) pilB 3277426..3279150 (+) 1725 WP_062384106.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AZF00_RS14495 (AZF00_14495) pilC 3279153..3280364 (+) 1212 WP_062384108.1 type II secretion system F family protein Machinery gene
  AZF00_RS14500 (AZF00_14500) pilD 3280401..3281285 (+) 885 WP_062384111.1 prepilin peptidase Machinery gene
  AZF00_RS14505 (AZF00_14505) coaE 3281312..3281920 (+) 609 WP_062384114.1 dephospho-CoA kinase -
  AZF00_RS14510 (AZF00_14510) - 3281954..3282157 (+) 204 WP_062384116.1 DNA gyrase inhibitor YacG -
  AZF00_RS14515 (AZF00_14515) mutT 3282185..3282607 (-) 423 WP_062384119.1 8-oxo-dGTP diphosphatase MutT -
  AZF00_RS14520 (AZF00_14520) argJ 3282588..3283808 (-) 1221 WP_062384121.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63182.58 Da        Isoelectric Point: 5.5802

>NTDB_id=172453 AZF00_RS14490 WP_062384106.1 3277426..3279150(+) (pilB) [Zhongshania aliphaticivorans strain SM-2]
MNSATIPLNGLARRLVYDAILDEETIRKAIEAAQKEKKSLVTHLVGKNLADGVRIAEIAADEFGIPFLDLRSYTTELAPQ
KIIDNKLIKAHRALPLWKRGKRLFIGMSDPTNLHALDQIKFHTGLTTEPIVVEESALNSAIEKYLSAQEESLADSLGDMD
DISLENLDIEAVDEDGGNGEVDLRGVDEAPVVKFVNKVLLDAIRGGASDIHFEPYEKIYRVRYRTDGILQEVARPPSTLG
YRLAARLKVMSQMDISERRVPQDGRIKMKLSKTRAIDFRVNTLPTLWGEKVVLRILDPSSAQMGIDALGYEPIQKDLYMK
ALNQPQGMILVTGPTGSGKTVSLYTGLNILNTPERNISTAEDPVEINLEGINQVHVNAKVGLGFPEALRSFLRQDPDIIM
VGEIRDLETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLLNMGVPAFNVATSVSLIIAQRLARRLCKECAIPADDVPKQT
LLDEGFSEELLKTAKIMHPVGCDHCNNGYKGRVGIYEVIRITPDISRIIMENGNSLAIASQAQAEGFNNLRQSALMKCAQ
GVTSLEEVNRVTKD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=172453 AZF00_RS14490 WP_062384106.1 3277426..3279150(+) (pilB) [Zhongshania aliphaticivorans strain SM-2]
ATGAATAGCGCAACGATCCCCTTAAACGGCCTAGCTCGACGACTGGTTTACGACGCAATACTTGATGAAGAAACCATTCG
CAAAGCAATTGAGGCTGCCCAGAAAGAAAAAAAGAGCCTAGTCACCCATCTTGTAGGGAAGAATTTGGCTGATGGGGTAC
GCATAGCGGAAATTGCCGCTGACGAGTTCGGCATTCCGTTTTTAGATCTGCGCTCCTACACCACAGAGCTAGCGCCCCAG
AAAATAATAGACAACAAACTAATTAAAGCCCACAGGGCTCTTCCTTTATGGAAACGGGGTAAGCGCTTATTTATCGGCAT
GTCGGATCCCACGAATCTACATGCCCTAGACCAAATTAAATTCCATACTGGACTAACCACGGAACCTATTGTTGTTGAAG
AGTCAGCGCTTAATAGCGCGATCGAAAAATATCTATCTGCCCAAGAGGAATCTCTTGCCGACAGCCTTGGCGACATGGAC
GATATCAGCCTTGAGAACTTAGACATTGAGGCGGTCGACGAAGACGGAGGCAATGGAGAGGTTGACCTTAGGGGTGTGGA
TGAGGCGCCCGTAGTTAAGTTTGTCAATAAGGTACTGCTTGACGCCATACGAGGTGGTGCTTCGGACATTCATTTCGAAC
CGTACGAAAAAATTTACCGCGTCCGTTACCGCACAGACGGTATTTTACAAGAGGTAGCGCGGCCGCCTTCTACACTCGGC
TATAGACTCGCGGCACGTCTAAAAGTTATGTCGCAAATGGACATCTCAGAGCGGCGCGTACCGCAGGATGGCCGAATTAA
GATGAAACTGTCTAAAACGCGGGCCATCGACTTCCGAGTTAACACTTTACCCACCCTGTGGGGCGAGAAGGTAGTACTAC
GGATACTCGATCCATCATCAGCGCAGATGGGCATTGACGCCTTGGGTTATGAGCCAATACAGAAAGATCTGTATATGAAA
GCACTAAACCAGCCCCAAGGTATGATTTTGGTCACGGGGCCAACTGGCTCCGGTAAAACTGTATCGCTTTATACCGGCTT
GAACATTCTCAACACCCCAGAAAGAAATATTTCCACTGCGGAAGACCCAGTCGAAATCAACCTCGAGGGCATTAACCAAG
TTCACGTCAACGCCAAGGTCGGTCTCGGCTTCCCCGAAGCGCTCCGCTCCTTTTTGCGACAAGACCCCGACATTATTATG
GTCGGTGAGATTCGCGACTTAGAAACTGCCGAAATAGCGATTAAAGCTGCGCAAACCGGTCACTTAGTACTATCTACACT
GCACACAAATAGTGCCCCAGAGACCTTAACACGCCTGCTCAATATGGGTGTACCGGCATTTAACGTCGCCACCTCTGTCA
GCTTAATTATTGCCCAGCGCTTAGCAAGACGACTGTGTAAGGAATGCGCCATTCCCGCCGACGATGTGCCAAAACAAACG
CTACTCGACGAAGGCTTTTCGGAAGAGCTGTTAAAAACCGCCAAAATAATGCACCCAGTAGGCTGCGACCATTGCAATAA
CGGCTACAAAGGCCGTGTTGGTATTTATGAAGTAATTCGCATCACCCCCGATATATCTCGTATTATTATGGAAAACGGTA
ATTCCTTAGCTATTGCCTCTCAAGCACAAGCAGAAGGTTTTAATAATTTACGTCAGTCTGCGCTAATGAAATGCGCACAG
GGCGTTACCAGCCTTGAAGAAGTCAACCGGGTTACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(197-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A127M869

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

60.208

100

0.606
  pilB Acinetobacter baumannii D1279779

60.702

99.303

0.603
  pilB Legionella pneumophila strain ERS1305867

56.567

99.477

0.563
  pilB Vibrio cholerae strain A1552

55.273

89.199

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

51.193

94.948

0.486
  pilB Vibrio campbellii strain DS40M4

50.459

94.948

0.479
  pilF Neisseria gonorrhoeae MS11

47.535

98.955

0.47
  pilB/pilB1 Synechocystis sp. PCC 6803

41.833

87.456

0.366

Multiple sequence alignment