Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   JT25_RS07425 Genome accession   NZ_CP014476
Coordinates   1602219..1603436 (-) Length   405 a.a.
NCBI ID   WP_036272829.1    Uniprot ID   -
Organism   Methylomonas denitrificans strain FJG1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1597219..1608436
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JT25_RS07395 (JT25_007395) argJ 1597505..1598719 (+) 1215 WP_052141966.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  JT25_RS07400 (JT25_007400) - 1598733..1599677 (+) 945 WP_036272834.1 Nudix family hydrolase -
  JT25_RS07405 (JT25_007405) yacG 1599692..1599895 (-) 204 WP_036272832.1 DNA gyrase inhibitor YacG -
  JT25_RS07410 (JT25_007410) zapD 1599895..1600668 (-) 774 WP_052141965.1 cell division protein ZapD -
  JT25_RS07415 (JT25_007415) coaE 1600665..1601276 (-) 612 WP_036272830.1 dephospho-CoA kinase -
  JT25_RS07420 (JT25_007420) pilD 1601280..1602146 (-) 867 WP_062328142.1 prepilin peptidase Machinery gene
  JT25_RS07425 (JT25_007425) pilC 1602219..1603436 (-) 1218 WP_036272829.1 type II secretion system F family protein Machinery gene
  JT25_RS07430 (JT25_007430) pilB 1603439..1605154 (-) 1716 WP_036272828.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JT25_RS07435 (JT25_007435) thiC 1605355..1607223 (-) 1869 WP_036272826.1 phosphomethylpyrimidine synthase ThiC -
  JT25_RS07440 (JT25_007440) - 1607621..1608025 (+) 405 WP_036272825.1 hypothetical protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44308.28 Da        Isoelectric Point: 6.7366

>NTDB_id=171800 JT25_RS07425 WP_036272829.1 1602219..1603436(-) (pilC) [Methylomonas denitrificans strain FJG1]
MAKQEEQIEFNWDGFDRQNKKTKGTISAKSEVIARAELRRMGIRVIGIKAKSKPLFGARVQKITPGDIAVFARQLATMLA
AGVPLVQSFDIIGKGHDNASMSELLLSIKADVEAGDTLAQALNRKPLYFDALFCNLVEAGEHAGVLETLLDKIATYKEKT
ESIKKKVKKALTYPIAVLVVAFIVTAILLIFVVPVFEDLFKGFGADLPAFTQFVIELSAWVQEWWWAVVGVIAIAAYVFN
YFKKRSKAFNHFLDKTLLKIPVVGMILEKSAIARFARTLSTMSAAGVPLVEALVSVAGACGNILFYEAVMKVRDDVSTGQ
QLKFALEATGMFPNMVVQMVAIGEESGSIDAMLDKVADFYEEEVDNLVDNLSSLMEPIIMVILGILVGGLIVAMYLPIFK
LGAAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=171800 JT25_RS07425 WP_036272829.1 1602219..1603436(-) (pilC) [Methylomonas denitrificans strain FJG1]
ATGGCAAAGCAAGAAGAGCAAATCGAGTTCAATTGGGACGGTTTCGATAGGCAAAACAAGAAAACCAAAGGCACCATCAG
CGCCAAGAGCGAGGTGATTGCCCGCGCCGAATTAAGGCGGATGGGCATCCGGGTAATCGGTATCAAAGCCAAGTCCAAGC
CCTTGTTCGGCGCCAGAGTGCAGAAAATAACGCCCGGCGACATCGCGGTATTCGCCAGGCAATTGGCGACGATGTTGGCC
GCTGGTGTGCCCTTGGTGCAATCTTTCGACATTATCGGCAAGGGGCACGACAATGCCAGCATGTCAGAGTTATTGCTATC
GATAAAAGCCGATGTGGAGGCTGGCGATACGTTGGCGCAGGCCCTAAACCGCAAACCACTGTATTTCGATGCCTTGTTTT
GCAATCTGGTCGAGGCCGGCGAACATGCTGGGGTATTGGAAACCTTGTTGGACAAGATTGCTACTTACAAGGAAAAAACC
GAATCGATCAAAAAGAAAGTTAAAAAAGCGTTGACCTACCCGATTGCGGTGTTGGTCGTGGCGTTTATCGTGACCGCCAT
TTTGCTGATTTTTGTGGTGCCGGTATTCGAGGATTTGTTTAAAGGTTTCGGTGCTGATTTGCCGGCCTTTACTCAGTTCG
TCATCGAGTTGTCGGCTTGGGTGCAGGAATGGTGGTGGGCAGTGGTCGGCGTTATCGCTATTGCTGCTTATGTATTCAAT
TATTTTAAAAAGCGTTCCAAGGCGTTTAACCATTTTCTCGATAAAACGCTACTGAAGATCCCGGTGGTCGGGATGATTCT
GGAAAAGTCGGCGATCGCCCGTTTCGCCAGAACCCTGTCCACGATGTCTGCGGCCGGTGTGCCCTTGGTCGAAGCGCTGG
TATCGGTTGCTGGAGCCTGCGGCAATATTTTATTCTATGAAGCGGTGATGAAGGTGCGCGACGACGTCTCCACCGGCCAA
CAATTGAAGTTTGCTTTGGAGGCTACCGGCATGTTTCCGAATATGGTGGTGCAGATGGTGGCGATCGGCGAAGAATCAGG
TTCTATAGACGCAATGCTGGATAAAGTTGCCGATTTTTACGAAGAAGAAGTCGATAACCTGGTCGATAACTTGAGTAGCT
TGATGGAGCCTATCATCATGGTAATCCTGGGCATCCTGGTCGGTGGCTTGATCGTCGCGATGTATCTGCCTATCTTCAAG
TTGGGCGCGGCTATTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

59.799

98.272

0.588
  pilC Pseudomonas stutzeri DSM 10701

58.333

97.778

0.57
  pilC Acinetobacter baylyi ADP1

52.696

100

0.531
  pilC Acinetobacter baumannii D1279779

54.156

98.025

0.531
  pilG Neisseria gonorrhoeae MS11

47.059

100

0.474
  pilG Neisseria meningitidis 44/76-A

47.059

100

0.474
  pilC Vibrio cholerae strain A1552

43.257

97.037

0.42
  pilC Vibrio campbellii strain DS40M4

40.863

97.284

0.398

Multiple sequence alignment