Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   JT25_RS07420 Genome accession   NZ_CP014476
Coordinates   1601280..1602146 (-) Length   288 a.a.
NCBI ID   WP_062328142.1    Uniprot ID   A0A126T2M6
Organism   Methylomonas denitrificans strain FJG1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1596280..1607146
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JT25_RS07385 (JT25_007385) - 1596322..1597068 (+) 747 WP_132324783.1 hypothetical protein -
  JT25_RS07395 (JT25_007395) argJ 1597505..1598719 (+) 1215 WP_052141966.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  JT25_RS07400 (JT25_007400) - 1598733..1599677 (+) 945 WP_036272834.1 Nudix family hydrolase -
  JT25_RS07405 (JT25_007405) yacG 1599692..1599895 (-) 204 WP_036272832.1 DNA gyrase inhibitor YacG -
  JT25_RS07410 (JT25_007410) zapD 1599895..1600668 (-) 774 WP_052141965.1 cell division protein ZapD -
  JT25_RS07415 (JT25_007415) coaE 1600665..1601276 (-) 612 WP_036272830.1 dephospho-CoA kinase -
  JT25_RS07420 (JT25_007420) pilD 1601280..1602146 (-) 867 WP_062328142.1 prepilin peptidase Machinery gene
  JT25_RS07425 (JT25_007425) pilC 1602219..1603436 (-) 1218 WP_036272829.1 type II secretion system F family protein Machinery gene
  JT25_RS07430 (JT25_007430) pilB 1603439..1605154 (-) 1716 WP_036272828.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 288 a.a.        Molecular weight: 31837.11 Da        Isoelectric Point: 7.1948

>NTDB_id=171799 JT25_RS07420 WP_062328142.1 1601280..1602146(-) (pilD) [Methylomonas denitrificans strain FJG1]
MVFATLQQYPLFLIGVVSVLGLLVGSFLNVVIYRLPVMMQAGWRRECQEYLKLPRDEAAEVFNLMLPASHCPSCKTEIKA
YQNIPVFSYLLLGGKCAHCGVKIAIRYPLIEAFTGLCSAVVAWHFGYGWALAFALPLTWCLIALSFIDIDQQLLPDSITL
PMLWLGLLLSLFDIYTNSYDAIIGVIAGYMSLWSVYQLFKLLTGKEGMGYGDFKLLALFGAWLGWQYLPLIILLSSLVGA
VVGIAMIVFGKRDAGKPIPFGPYLAMAGWLAMLFGPELNALYLHTAGL

Nucleotide


Download         Length: 867 bp        

>NTDB_id=171799 JT25_RS07420 WP_062328142.1 1601280..1602146(-) (pilD) [Methylomonas denitrificans strain FJG1]
ATGGTTTTTGCCACTCTACAACAATATCCGCTGTTCCTGATCGGCGTCGTTAGCGTGCTGGGCCTATTGGTCGGTAGTTT
TCTAAACGTGGTGATCTACCGCTTGCCTGTAATGATGCAAGCCGGTTGGCGGCGTGAGTGTCAAGAATATCTAAAGTTGC
CGCGGGACGAAGCGGCTGAAGTGTTCAACTTGATGTTGCCGGCCTCACATTGTCCGTCTTGCAAAACCGAGATCAAAGCC
TATCAAAATATCCCGGTGTTCAGCTATTTGCTGTTGGGCGGTAAATGCGCACATTGCGGGGTGAAAATCGCGATCCGTTA
TCCGCTCATCGAGGCGTTTACCGGCCTGTGTTCTGCGGTCGTGGCTTGGCATTTCGGATACGGTTGGGCTTTAGCGTTTG
CATTGCCGTTGACTTGGTGTTTGATTGCATTGAGTTTTATCGACATCGATCAGCAATTGTTACCAGATTCGATCACTCTG
CCGATGCTGTGGCTGGGTTTGTTGCTAAGCCTGTTTGATATTTACACTAATAGCTATGACGCCATTATTGGCGTGATCGC
TGGTTATATGAGCCTATGGAGTGTTTACCAACTGTTCAAACTGCTGACAGGTAAGGAAGGCATGGGCTACGGCGATTTCA
AGCTGCTGGCGCTGTTCGGTGCCTGGCTGGGCTGGCAATATCTGCCGCTGATTATTTTATTGTCATCGCTGGTGGGGGCG
GTCGTCGGCATTGCGATGATCGTGTTCGGCAAGCGCGATGCCGGCAAACCGATACCGTTCGGGCCGTATCTGGCCATGGC
CGGCTGGCTGGCGATGTTGTTCGGACCGGAGCTGAATGCCTTATATCTGCATACTGCCGGCCTGTAG

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(137-244)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(19-125)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A126T2M6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter baumannii D1279779

54.275

93.403

0.507
  pilD Acinetobacter nosocomialis M2

53.903

93.403

0.503
  pilD Vibrio cholerae strain A1552

51.418

97.917

0.503
  pilD Vibrio campbellii strain DS40M4

51.246

97.569

0.5
  pilD Neisseria gonorrhoeae MS11

53.077

90.278

0.479

Multiple sequence alignment