Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JT25_RS07430 Genome accession   NZ_CP014476
Coordinates   1603439..1605154 (-) Length   571 a.a.
NCBI ID   WP_036272828.1    Uniprot ID   -
Organism   Methylomonas denitrificans strain FJG1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1598439..1610154
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JT25_RS07400 (JT25_007400) - 1598733..1599677 (+) 945 WP_036272834.1 Nudix family hydrolase -
  JT25_RS07405 (JT25_007405) yacG 1599692..1599895 (-) 204 WP_036272832.1 DNA gyrase inhibitor YacG -
  JT25_RS07410 (JT25_007410) zapD 1599895..1600668 (-) 774 WP_052141965.1 cell division protein ZapD -
  JT25_RS07415 (JT25_007415) coaE 1600665..1601276 (-) 612 WP_036272830.1 dephospho-CoA kinase -
  JT25_RS07420 (JT25_007420) pilD 1601280..1602146 (-) 867 WP_062328142.1 prepilin peptidase Machinery gene
  JT25_RS07425 (JT25_007425) pilC 1602219..1603436 (-) 1218 WP_036272829.1 type II secretion system F family protein Machinery gene
  JT25_RS07430 (JT25_007430) pilB 1603439..1605154 (-) 1716 WP_036272828.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JT25_RS07435 (JT25_007435) thiC 1605355..1607223 (-) 1869 WP_036272826.1 phosphomethylpyrimidine synthase ThiC -
  JT25_RS07440 (JT25_007440) - 1607621..1608025 (+) 405 WP_036272825.1 hypothetical protein -
  JT25_RS07445 (JT25_007445) ispB 1608030..1609034 (-) 1005 WP_052141964.1 octaprenyl diphosphate synthase -
  JT25_RS07450 (JT25_007450) rplU 1609241..1609552 (+) 312 WP_036272927.1 50S ribosomal protein L21 -
  JT25_RS07455 (JT25_007455) rpmA 1609570..1609827 (+) 258 WP_020484554.1 50S ribosomal protein L27 -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63274.93 Da        Isoelectric Point: 6.1194

>NTDB_id=171801 JT25_RS07430 WP_036272828.1 1603439..1605154(-) (pilB) [Methylomonas denitrificans strain FJG1]
MATAPSNIHFSGLVKCLIQKGLLTEESAATCLQEAQKRKQAFISYLVEHKYADSKTVATLASAEFGVPFFDLDAIDPSFL
PIKLVSEKLIRQHHALPLFKRGNRLFVATADPTNFQALDEIKFHTRHNTENILVEEDKLIRMIDTALEAADTSMKDLLDD
DLDNIDISAGDDSKPADETMGSDIDDAPIVRFVNKILLDSIKRGVSDIHMEPFEKKFRIRFRADGILSEVASPPVGIANR
IISRLKVMSRMDIAERRVPQDGRIKMQISKNRAIDFRVNTCPTLFGEKVVMRILDPTSAQIGIEKLGFEPEQQKNFLEAI
HKPYGMVLVTGPTGSGKTVSLYTGLNILNSIETNISTAEDPVEITVEGINQVNVNPKAGLTFAEALRAFLRQDPDIIMVG
EIRDLETASIAVKAAQTGHMVLSTLHTNDAPQTINRLVQMGIEPFNIVSAVVLIMAQRLARRLCEHCKQEVDYPESALLS
AGFKQEEIGTFKVYGPVGCEHCNNGYKGRVGIYQVMPITEKMRALILNGGNSMQMAELSKSEGINDLRMSGLLKIKQGIT
SLQEIDRVTKE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=171801 JT25_RS07430 WP_036272828.1 1603439..1605154(-) (pilB) [Methylomonas denitrificans strain FJG1]
ATGGCGACAGCACCTTCTAACATCCATTTCAGCGGCTTGGTCAAATGCCTGATTCAGAAAGGTTTGTTGACCGAGGAATC
GGCGGCAACCTGCCTGCAGGAGGCGCAGAAACGCAAACAAGCGTTCATAAGCTATTTGGTTGAGCATAAGTATGCGGACA
GCAAAACCGTGGCTACGTTGGCTTCGGCGGAGTTTGGCGTGCCGTTTTTCGATCTGGATGCGATTGATCCGTCGTTCTTG
CCGATCAAGTTGGTTAGCGAAAAATTGATTCGTCAACATCACGCCTTGCCCTTGTTCAAGCGCGGCAACCGTCTGTTCGT
CGCAACTGCGGATCCGACCAATTTTCAGGCGCTGGACGAGATCAAGTTCCATACCCGGCACAATACCGAAAATATTCTGG
TCGAAGAAGACAAACTGATCAGAATGATAGACACGGCGCTGGAAGCCGCCGATACGTCGATGAAGGATCTGCTGGATGAC
GATCTGGATAATATCGATATTTCCGCGGGCGACGACAGCAAGCCCGCCGACGAAACAATGGGCTCGGATATCGACGACGC
GCCTATCGTTCGCTTTGTGAATAAGATTTTGTTGGATTCGATTAAGCGTGGTGTGTCGGATATACACATGGAACCCTTCG
AGAAAAAATTCCGCATCCGCTTTCGGGCCGACGGTATCTTGTCGGAGGTGGCCAGTCCGCCGGTGGGTATTGCCAACCGG
ATTATTTCCAGGCTGAAAGTCATGTCGAGAATGGACATCGCCGAACGGCGGGTACCGCAGGATGGCCGGATCAAGATGCA
GATTTCCAAGAATCGGGCCATCGATTTTCGGGTTAACACCTGTCCGACGCTGTTCGGTGAAAAAGTGGTGATGCGGATTC
TCGATCCAACCAGTGCCCAGATCGGTATCGAGAAGCTCGGTTTCGAACCCGAGCAACAGAAAAACTTTTTGGAAGCGATT
CACAAGCCTTACGGCATGGTGTTGGTCACCGGGCCGACGGGTAGTGGTAAAACCGTATCGCTGTATACCGGGCTGAATAT
CCTAAACAGCATCGAAACCAATATTTCCACAGCCGAAGATCCCGTCGAGATCACCGTGGAAGGCATCAATCAGGTTAACG
TCAATCCCAAGGCCGGCCTGACCTTTGCCGAGGCGTTACGGGCGTTTTTGCGGCAAGACCCGGACATCATCATGGTCGGC
GAGATTCGCGACCTGGAAACCGCCAGCATAGCGGTCAAGGCCGCGCAAACCGGTCACATGGTGTTATCCACGCTGCACAC
CAATGACGCGCCGCAAACCATCAACCGTCTGGTGCAGATGGGCATAGAGCCCTTCAATATCGTTTCCGCTGTGGTTTTGA
TCATGGCGCAGCGGCTGGCTCGGCGCTTGTGTGAGCATTGCAAGCAGGAAGTCGATTACCCTGAAAGCGCCTTGCTGTCG
GCCGGGTTTAAACAGGAGGAAATCGGTACGTTTAAAGTATACGGTCCGGTGGGTTGCGAACATTGCAATAACGGTTACAA
GGGCAGGGTAGGTATCTATCAGGTGATGCCTATTACCGAAAAAATGCGCGCGCTGATTTTGAACGGCGGCAACTCCATGC
AAATGGCGGAATTGTCGAAAAGTGAAGGGATTAACGATTTAAGAATGTCCGGGCTGTTAAAAATAAAGCAAGGCATTACC
TCGCTGCAAGAAATTGACCGGGTCACCAAGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

55.497

100

0.557
  pilB Acinetobacter baumannii D1279779

53.229

100

0.534
  pilB Acinetobacter baylyi ADP1

52.837

98.774

0.522
  pilF Neisseria gonorrhoeae MS11

48.925

97.723

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

46.181

98.599

0.455
  pilB Vibrio campbellii strain DS40M4

45.471

98.599

0.448
  pilB Vibrio cholerae strain A1552

49.803

88.967

0.443
  pilF Thermus thermophilus HB27

39.568

97.373

0.385
  pilB/pilB1 Synechocystis sp. PCC 6803

39.344

96.147

0.378
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.462

97.898

0.377

Multiple sequence alignment