Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AYM39_RS00790 Genome accession   NZ_CP014360
Coordinates   179772..181487 (-) Length   571 a.a.
NCBI ID   WP_064020099.1    Uniprot ID   -
Organism   Methylomonas sp. DH-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 174772..186487
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AYM39_RS00760 (AYM39_00755) - 175217..176002 (-) 786 WP_064020093.1 class I SAM-dependent methyltransferase -
  AYM39_RS00765 (AYM39_00760) - 176025..176240 (-) 216 WP_064020094.1 DNA gyrase inhibitor YacG -
  AYM39_RS00770 (AYM39_00765) zapD 176240..177013 (-) 774 WP_064020095.1 cell division protein ZapD -
  AYM39_RS00775 (AYM39_00770) coaE 177010..177621 (-) 612 WP_064020096.1 dephospho-CoA kinase -
  AYM39_RS00780 (AYM39_00775) pilD 177625..178491 (-) 867 WP_064020097.1 A24 family peptidase Machinery gene
  AYM39_RS00785 (AYM39_00780) pilC 178552..179769 (-) 1218 WP_064020098.1 type II secretion system F family protein Machinery gene
  AYM39_RS00790 (AYM39_00785) pilB 179772..181487 (-) 1716 WP_064020099.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AYM39_RS00795 (AYM39_00790) thiC 181650..183518 (-) 1869 WP_064020100.1 phosphomethylpyrimidine synthase ThiC -
  AYM39_RS00800 (AYM39_00795) recD 183813..185453 (+) 1641 WP_064020101.1 exodeoxyribonuclease V subunit alpha -
  AYM39_RS00805 (AYM39_00800) - 185450..185863 (+) 414 WP_064020102.1 hypothetical protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63213.85 Da        Isoelectric Point: 6.1220

>NTDB_id=170651 AYM39_RS00790 WP_064020099.1 179772..181487(-) (pilB) [Methylomonas sp. DH-1]
MATAPSNIHFSGLVKCLIQKGLLTEESAATCLQEAQKRKQAFISYLVEHKYADSKTIATLASAEFGVPFLDLDAIDPSFL
PIKLVSEKLIRQHHALPLFKRGNRLFVATADPTNFQALDEIKFHTRHNTENILVEEDKLMKMIDTALEAADTSMKDLLDD
DLDNIDISAGDEAKPAEETSGSDIDDAPIVRFVNKILLDSIKRGVSDIHMEPFEKRFRIRFRADGILSEVASPPVGIANR
IISRLKVMSKMDIAERRVPQDGRIKMQISKNRAIDFRVNTCPTLFGEKVVLRILDPTSAQIGIEKLGFEPEQQKNFLDAI
HKPYGMVLVTGPTGSGKTVSLYTGLNILNSVETNISTAEDPVEITVEGINQVNVNPKAGLTFAEALKAFLRQDPDIIMVG
EIRDYETASIAVKAAQTGHLVLSTLHTNDAPQTINRLVQMGIEPFNIVSAVLLIMAQRLARRLCEHCKKEMDYPETALIS
AGFKKEDIGTFKIYGPVGCEHCNNGYKGRVGIYQVMPITEKMRALILRGGNAMEMAELSRSEGINDLRASGLLKVRQGIT
SLEEIDRVTTE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=170651 AYM39_RS00790 WP_064020099.1 179772..181487(-) (pilB) [Methylomonas sp. DH-1]
ATGGCGACAGCACCTTCCAACATTCATTTCAGCGGCCTGGTTAAGTGCCTGATTCAAAAGGGACTGCTGACCGAAGAGTC
GGCCGCGACCTGTTTGCAGGAGGCGCAAAAACGCAAACAGGCGTTTATTAGCTATCTGGTCGAACATAAATACGCGGATA
GTAAAACCATCGCCACGCTGGCCTCGGCCGAGTTCGGCGTGCCGTTTCTCGACCTGGACGCGATCGATCCGTCGTTCCTG
CCGATCAAACTGGTCAGCGAAAAATTGATTCGCCAGCACCACGCATTGCCCTTGTTCAAACGCGGCAACCGCTTGTTCGT
GGCGACGGCCGATCCGACCAATTTCCAGGCCCTGGACGAGATCAAGTTCCACACTCGGCACAACACCGAGAACATCCTGG
TCGAAGAAGACAAATTGATGAAGATGATCGATACCGCATTGGAAGCGGCCGATACATCGATGAAGGATTTGCTCGACGAC
GATCTGGACAACATCGACATTTCCGCCGGCGATGAAGCTAAGCCGGCCGAGGAAACTTCCGGTTCGGATATCGACGACGC
GCCTATCGTACGTTTCGTCAACAAGATTCTGCTGGACTCGATCAAGCGCGGCGTATCGGATATTCACATGGAACCGTTCG
AGAAGCGGTTCCGCATCCGCTTCCGCGCCGACGGCATTCTGTCGGAAGTAGCCAGTCCGCCGGTGGGCATCGCCAACCGG
ATCATTTCCCGGCTGAAAGTCATGTCGAAAATGGACATCGCCGAGCGGCGGGTACCGCAGGACGGCCGGATCAAGATGCA
GATCTCGAAAAACCGCGCCATCGATTTCCGGGTCAATACCTGTCCGACCTTGTTCGGCGAAAAAGTGGTGTTGCGGATTC
TCGATCCGACCAGCGCCCAAATCGGTATCGAAAAACTCGGGTTCGAACCGGAACAACAGAAAAATTTCCTCGATGCGATT
CATAAACCCTACGGCATGGTATTGGTCACCGGACCGACCGGTAGCGGTAAGACCGTGTCGCTGTACACCGGTTTGAACAT
ACTGAACAGCGTCGAGACCAATATTTCCACGGCCGAAGACCCGGTCGAGATCACCGTGGAAGGCATCAACCAGGTCAACG
TCAACCCCAAAGCCGGTTTGACCTTCGCCGAAGCCCTCAAGGCGTTCTTGCGGCAAGACCCGGATATCATCATGGTCGGC
GAGATTCGCGATTACGAAACCGCCAGTATCGCGGTCAAAGCGGCGCAAACCGGCCACTTGGTGTTGTCGACGCTGCACAC
CAACGACGCGCCGCAGACTATTAACCGCTTGGTGCAAATGGGTATCGAGCCGTTCAACATCGTGTCGGCGGTTTTGCTGA
TCATGGCGCAACGGCTGGCACGGCGCTTGTGCGAACATTGTAAAAAGGAAATGGACTATCCGGAAACTGCGCTAATCTCG
GCTGGCTTCAAAAAAGAGGACATCGGTACCTTCAAAATATATGGGCCGGTGGGCTGCGAACATTGCAATAACGGCTATAA
AGGGCGGGTCGGTATTTACCAAGTGATGCCGATCACCGAAAAAATGCGGGCCTTGATCCTGCGCGGCGGCAATGCGATGG
AAATGGCCGAATTGTCACGCAGCGAAGGCATCAACGATTTGCGCGCCTCCGGTTTGTTGAAAGTCCGCCAGGGCATTACC
TCGCTGGAAGAAATTGACCGCGTCACCACGGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-147)

Type II/IV secretion system protein (T2SSE)

(195-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

55.478

100

0.559
  pilB Acinetobacter baumannii D1279779

53.054

100

0.532
  pilB Acinetobacter baylyi ADP1

53.191

98.774

0.525
  pilF Neisseria gonorrhoeae MS11

47.849

97.723

0.468
  pilB Vibrio parahaemolyticus RIMD 2210633

46.085

98.424

0.454
  pilB Vibrio campbellii strain DS40M4

45.133

98.949

0.447
  pilB Vibrio cholerae strain A1552

49.409

88.967

0.44
  pilF Thermus thermophilus HB27

39.68

98.424

0.391
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.104

97.898

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

39.011

95.622

0.373

Multiple sequence alignment