Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   AYM39_RS00780 Genome accession   NZ_CP014360
Coordinates   177625..178491 (-) Length   288 a.a.
NCBI ID   WP_064020097.1    Uniprot ID   A0A172U3W5
Organism   Methylomonas sp. DH-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 172625..183491
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AYM39_RS00750 (AYM39_00745) argJ 173018..174232 (+) 1215 WP_064020091.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  AYM39_RS00755 (AYM39_00750) - 174240..175178 (+) 939 WP_064020092.1 Nudix family hydrolase -
  AYM39_RS00760 (AYM39_00755) - 175217..176002 (-) 786 WP_064020093.1 class I SAM-dependent methyltransferase -
  AYM39_RS00765 (AYM39_00760) - 176025..176240 (-) 216 WP_064020094.1 DNA gyrase inhibitor YacG -
  AYM39_RS00770 (AYM39_00765) zapD 176240..177013 (-) 774 WP_064020095.1 cell division protein ZapD -
  AYM39_RS00775 (AYM39_00770) coaE 177010..177621 (-) 612 WP_064020096.1 dephospho-CoA kinase -
  AYM39_RS00780 (AYM39_00775) pilD 177625..178491 (-) 867 WP_064020097.1 A24 family peptidase Machinery gene
  AYM39_RS00785 (AYM39_00780) pilC 178552..179769 (-) 1218 WP_064020098.1 type II secretion system F family protein Machinery gene
  AYM39_RS00790 (AYM39_00785) pilB 179772..181487 (-) 1716 WP_064020099.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 288 a.a.        Molecular weight: 31534.60 Da        Isoelectric Point: 7.4393

>NTDB_id=170649 AYM39_RS00780 WP_064020097.1 177625..178491(-) (pilD) [Methylomonas sp. DH-1]
MLFNALAQSAGLLTGSALVLGLLVGSFLNVVIYRLPLMMQAGWRRECREYLELPAEAPAERFDLLLPASHCPACKAEIKA
YQNIPVFSYLLLGGKCANCGAKIAWRYPLIEAFTGLCSALVAWQLGYGWALAFALPLTWCLIALSFIDIDQQLLPDSITL
PLVWLGLLLSVFGIFADSRDSIIGAVAGYLSLWCVFHGFKLLTGKEGMGYGDFKLLAVFGAWLGWQYLPLIVLLSSLVGA
TVGIAMIVFCRRDAAKPIPFGPYLAMAGWLALLWGEQMNAWYLRITGL

Nucleotide


Download         Length: 867 bp        

>NTDB_id=170649 AYM39_RS00780 WP_064020097.1 177625..178491(-) (pilD) [Methylomonas sp. DH-1]
ATGTTGTTTAACGCCTTAGCCCAATCCGCCGGTCTTCTGACCGGCAGCGCTTTAGTACTCGGCCTGTTGGTCGGCAGCTT
TCTGAACGTGGTGATTTACCGGCTGCCGCTGATGATGCAGGCCGGTTGGCGCCGAGAGTGCCGCGAGTACCTGGAATTGC
CGGCGGAAGCTCCGGCCGAACGTTTCGACTTGTTGCTGCCGGCTTCGCATTGCCCGGCTTGTAAAGCGGAAATCAAAGCC
TACCAAAACATCCCGGTCTTCAGTTACCTGCTGCTGGGCGGCAAATGCGCCAACTGCGGCGCGAAAATCGCTTGGCGCTA
CCCGCTGATCGAAGCCTTTACCGGCCTGTGTTCGGCGCTGGTGGCCTGGCAACTGGGTTACGGTTGGGCCTTGGCCTTTG
CCTTGCCTTTGACTTGGTGCCTGATTGCGTTGAGCTTTATCGACATCGACCAACAATTGTTGCCCGATTCCATCACGCTG
CCCTTGGTGTGGTTGGGCTTGCTGCTCAGCGTATTCGGCATTTTTGCCGACAGCCGCGACAGTATTATCGGTGCGGTAGC
CGGCTATTTGAGCCTCTGGTGCGTATTCCACGGCTTCAAATTGCTGACCGGCAAGGAAGGCATGGGCTACGGCGATTTCA
AATTGCTGGCCGTATTCGGCGCTTGGCTGGGCTGGCAATATCTACCTTTGATCGTATTGTTGTCTTCCCTGGTCGGCGCA
ACGGTCGGCATAGCAATGATCGTGTTCTGCCGGCGCGATGCGGCCAAGCCGATCCCGTTCGGGCCTTACTTGGCAATGGC
CGGCTGGCTGGCCTTGCTGTGGGGCGAGCAAATGAATGCCTGGTATCTGCGAATTACGGGCCTGTGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(137-244)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(19-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A172U3W5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio campbellii strain DS40M4

54.647

93.403

0.51
  pilD Acinetobacter baumannii D1279779

53.358

93.056

0.497
  pilD Acinetobacter nosocomialis M2

52.612

93.056

0.49
  pilD Vibrio cholerae strain A1552

51.673

93.403

0.483
  pilD Neisseria gonorrhoeae MS11

52.941

88.542

0.469

Multiple sequence alignment