Detailed information NTG0169697

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AXE82_RS05675
• Genome accession: NZ_CP014234
• Coordinates: 1300635..1301510 (-)
• Length: 291 a.a.
• NCBI ID: WP_062332387.1
• Uniprot ID: A0A378Q6Z4
• Organism: Moraxella osloensis strain CCUG 350
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1295635..1306510

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AXE82_RS05655 (AXE82_05655)	nfuA	1296431..1297048 (-)	618	WP_062332384.1	Fe-S biogenesis protein NfuA	-
AXE82_RS05660 (AXE82_05660)	-	1297346..1298449 (+)	1104	WP_082741424.1	bifunctional nicotinamide-nucleotide adenylyltransferase/Nudix hydroxylase	-
AXE82_RS05665 (AXE82_05665)	-	1298518..1299924 (+)	1407	WP_062332386.1	nicotinate phosphoribosyltransferase	-
AXE82_RS05670 (AXE82_05670)	coaE	1299982..1300638 (-)	657	WP_227713376.1	dephospho-CoA kinase	-
AXE82_RS05675 (AXE82_05675)	pilD	1300635..1301510 (-)	876	WP_062332387.1	prepilin peptidase	Machinery gene
AXE82_RS05680 (AXE82_05680)	pilC	1301516..1302739 (-)	1224	WP_062332389.1	type II secretion system F family protein	Machinery gene
AXE82_RS05685 (AXE82_05685)	pilB	1302796..1304502 (-)	1707	WP_062332391.1	type IV-A pilus assembly ATPase PilB	Machinery gene
AXE82_RS05690 (AXE82_05690)	tpiA	1304936..1305790 (+)	855	WP_062332394.1	triose-phosphate isomerase	-
AXE82_RS05695 (AXE82_05695)	secG	1305881..1306201 (+)	321	WP_062332397.1	preprotein translocase subunit SecG	-

Sequence

Protein

• Length: 291 a.a.
• Molecular weight: 32289.78 Da
• Isoelectric Point: 8.1744

>NTDB_id=169697 AXE82_RS05675 WP_062332387.1 1300635..1301510(-) (pilD) [Moraxella osloensis strain CCUG 350]
MGIFSDFILLLQQSQWLTLIIVALLGLCVGSFLNVVIYRTPLMMEQQWKNECQLLLHPETIVDHHESITLSRPVSRCPHC
GHSIRWFENMPVISWLLLKGKCSQCHHAISYRYPLVEIITAILSVLVVMKFGVTWQALAGLVLTWTLVALTGIDFDTQLL
PDRFTLPLAGLGLFVNAFGLFVSPTAAIFGYVFGFLCLWIVYQLFLLITGKHGMGHGDFKLLAALGAWLGPLMLPLIVLL
SAFVGSVVGVILMKRSGESKPFAFGPYIAIAGMIALLYGQSIMSWYLGKMS

Nucleotide

• Length: 876 bp

>NTDB_id=169697 AXE82_RS05675 WP_062332387.1 1300635..1301510(-) (pilD) [Moraxella osloensis strain CCUG 350]
ATGGGGATTTTTTCAGATTTTATTTTGTTGTTACAACAGTCACAATGGCTGACGTTGATTATCGTAGCTTTATTGGGATT
ATGCGTGGGCAGTTTTCTCAATGTGGTGATTTATCGCACGCCTTTGATGATGGAGCAGCAGTGGAAGAACGAATGCCAGC
TATTGCTTCATCCAGAAACCATTGTTGACCATCATGAATCCATTACCTTAAGCCGTCCTGTTTCTCGTTGTCCGCATTGT
GGGCATTCAATACGTTGGTTTGAAAATATGCCTGTTATTAGCTGGCTACTGCTAAAAGGAAAATGTAGTCAGTGTCATCA
CGCCATTAGTTATCGTTATCCGCTTGTAGAAATCATCACCGCTATTTTGTCTGTGCTGGTGGTGATGAAATTTGGTGTTA
CTTGGCAAGCGCTGGCAGGATTGGTACTCACATGGACATTAGTGGCTTTGACAGGCATTGATTTTGATACCCAATTGTTG
CCTGACCGATTTACATTGCCGTTAGCGGGATTAGGCTTATTTGTCAATGCCTTTGGCTTGTTTGTCAGTCCCACAGCGGC
TATTTTTGGCTATGTGTTTGGGTTTTTATGCTTGTGGATTGTCTACCAACTGTTTTTGCTGATTACGGGCAAGCACGGGA
TGGGGCATGGCGATTTTAAATTATTAGCGGCTTTGGGGGCTTGGCTAGGGCCTTTGATGCTACCGTTAATTGTGCTTTTA
TCTGCATTTGTCGGCTCGGTTGTGGGTGTAATATTGATGAAGCGCTCAGGTGAGAGTAAACCTTTTGCTTTTGGACCCTA
TATCGCCATTGCTGGCATGATCGCACTTTTGTATGGGCAATCAATAATGTCGTGGTATCTAGGCAAAATGTCATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (24-131)

• Type IV leader peptidase family (Peptidase_A24) (141-250)

3D structure

• AlphaFold DB: A0A378Q6Z4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter baumannii D1279779	63.028	97.595	0.615
pilD	Acinetobacter nosocomialis M2	63.028	97.595	0.615
pilD	Vibrio campbellii strain DS40M4	48.754	96.564	0.471
pilD	Vibrio cholerae strain A1552	49.27	94.158	0.464
pilD	Neisseria gonorrhoeae MS11	45.185	92.784	0.419