Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AXE82_RS05685 Genome accession   NZ_CP014234
Coordinates   1302796..1304502 (-) Length   568 a.a.
NCBI ID   WP_062332391.1    Uniprot ID   -
Organism   Moraxella osloensis strain CCUG 350     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1297796..1309502
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AXE82_RS05665 (AXE82_05665) - 1298518..1299924 (+) 1407 WP_062332386.1 nicotinate phosphoribosyltransferase -
  AXE82_RS05670 (AXE82_05670) coaE 1299982..1300638 (-) 657 WP_227713376.1 dephospho-CoA kinase -
  AXE82_RS05675 (AXE82_05675) pilD 1300635..1301510 (-) 876 WP_062332387.1 prepilin peptidase Machinery gene
  AXE82_RS05680 (AXE82_05680) pilC 1301516..1302739 (-) 1224 WP_062332389.1 type II secretion system F family protein Machinery gene
  AXE82_RS05685 (AXE82_05685) pilB 1302796..1304502 (-) 1707 WP_062332391.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AXE82_RS05690 (AXE82_05690) tpiA 1304936..1305790 (+) 855 WP_062332394.1 triose-phosphate isomerase -
  AXE82_RS05695 (AXE82_05695) secG 1305881..1306201 (+) 321 WP_062332397.1 preprotein translocase subunit SecG -
  AXE82_RS05710 (AXE82_05710) rimP 1306765..1307274 (+) 510 WP_062332400.1 ribosome maturation factor RimP -
  AXE82_RS05715 (AXE82_05715) nusA 1307392..1308873 (+) 1482 WP_062332403.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62612.12 Da        Isoelectric Point: 5.2322

>NTDB_id=169699 AXE82_RS05685 WP_062332391.1 1302796..1304502(-) (pilB) [Moraxella osloensis strain CCUG 350]
MAAPLPSFGGIASRLVHEGLISEAAMQKVLAESQQQKVNLIAYVVDNKLAQADAVAWVISREFGDPLIDLDAINVNYIPK
EAVDEKIIREFNILPIFQRGSRLFVAMSDPTRVDALDALRFGTRFTVETVVAEHHKIKALIEKIFTTTNLADFDDMDAVE
FDETRADEDSSATSHLDINDEAPVVKFVNGMLIKAITMGASDLHFEPYEKSYRVRFRIDGVLQKMANPPTQLAGKIAARL
KVMSQMDISERRVPQDGRIKLKISKDKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPDQKQLFMDALAKPQG
MLLITGPTGSGKTVSLYTGLNILNTVETNISTAEDPVEINLEGINQVNVNNKVGLTFASALKSFLRQDPDIVMVGEIRDL
ETAEIAVKAAQTGHMVLSTLHTNSAPETLTRLRNMGVASFNIATSVNLVIAQRLARRLCKACKKPTDVPKNSLLEMGFTE
EDLADPSNVIYEPVGCAECRDGYKGRVGIYEVMKVTPEIARIIMEDGNAIEIKDAALKQGFRDLRRSGILKVLQGTTSIQ
EMYRVTSE

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=169699 AXE82_RS05685 WP_062332391.1 1302796..1304502(-) (pilB) [Moraxella osloensis strain CCUG 350]
ATGGCGGCACCCCTTCCTAGTTTTGGTGGCATTGCCTCGCGATTAGTGCATGAAGGACTAATTAGCGAAGCGGCAATGCA
AAAAGTATTGGCAGAATCGCAGCAGCAAAAAGTAAATTTAATCGCCTATGTGGTTGATAATAAGCTTGCACAAGCTGACG
CGGTAGCGTGGGTGATTTCTCGAGAATTTGGCGACCCACTCATTGATTTGGATGCCATCAATGTCAACTATATTCCAAAA
GAAGCGGTTGATGAAAAAATTATTCGCGAATTTAATATTCTGCCGATTTTCCAACGAGGTAGTCGCTTATTTGTTGCCAT
GAGTGACCCAACGCGGGTAGATGCTTTAGACGCGCTGCGGTTTGGTACAAGGTTTACGGTAGAAACCGTGGTTGCCGAAC
ACCACAAAATCAAAGCACTCATCGAAAAAATCTTTACTACCACTAATTTAGCTGATTTTGATGATATGGATGCGGTGGAG
TTTGATGAAACCCGCGCCGATGAAGACAGTAGTGCCACCAGTCATTTAGATATCAATGATGAAGCCCCTGTCGTCAAGTT
CGTTAATGGGATGCTCATCAAAGCCATTACCATGGGCGCCTCGGACTTACACTTTGAACCCTATGAAAAATCTTATCGCG
TGCGTTTTCGTATTGATGGGGTGTTACAAAAAATGGCAAATCCACCGACGCAGCTAGCGGGTAAAATTGCTGCCCGCCTC
AAAGTCATGTCACAAATGGACATCTCTGAGCGCCGCGTGCCGCAAGATGGTCGTATCAAGTTAAAAATTTCCAAGGATAA
GGCGATTGATTTTCGTGTCAACTCATTGCCAACCTTATTTGGTGAAAAAATTGTACTGCGTATTTTAGACCCCTCATCAG
CGATGCTAGGCATTGATGCACTGGGCTATGAGCCTGACCAAAAGCAGCTATTTATGGATGCGCTGGCAAAACCGCAAGGG
ATGTTATTGATTACCGGTCCTACTGGTTCGGGTAAAACCGTATCCTTGTATACGGGGCTTAATATCTTAAACACAGTTGA
GACCAATATTTCCACCGCAGAAGATCCCGTTGAAATTAACCTTGAAGGCATCAATCAGGTCAACGTGAATAATAAAGTTG
GCTTGACATTTGCCAGTGCGCTCAAATCATTCTTGCGTCAAGACCCTGATATTGTGATGGTCGGTGAGATTCGTGACTTA
GAAACGGCGGAAATTGCTGTCAAAGCTGCCCAAACAGGGCATATGGTACTGTCAACCTTGCATACCAACTCAGCGCCAGA
AACCTTAACGCGTCTACGTAATATGGGGGTGGCATCCTTTAACATCGCCACCTCGGTCAATTTGGTAATCGCACAGCGCT
TAGCCCGTCGCCTGTGTAAAGCTTGTAAGAAACCGACAGATGTACCAAAAAATAGCTTGCTTGAAATGGGCTTTACGGAA
GAAGATTTGGCAGATCCCAGCAATGTCATTTATGAGCCTGTTGGCTGCGCTGAATGCCGCGATGGCTATAAAGGACGTGT
TGGTATTTATGAAGTGATGAAAGTAACACCAGAGATTGCCCGAATTATTATGGAAGACGGCAACGCTATTGAGATTAAAG
ACGCTGCCTTAAAGCAAGGGTTTAGGGATTTAAGGCGCTCTGGCATTTTAAAGGTTCTACAAGGCACGACCAGTATCCAA
GAGATGTATCGTGTTACTAGCGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(15-145)

Type II/IV secretion system protein (T2SSE)

(191-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

68.021

99.648

0.678
  pilB Acinetobacter baylyi ADP1

65.608

99.824

0.655
  pilB Legionella pneumophila strain ERS1305867

51.15

99.472

0.509
  pilF Neisseria gonorrhoeae MS11

49.195

98.415

0.484
  pilB Vibrio cholerae strain A1552

49.016

98.415

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

47.1

100

0.472
  pilB Vibrio campbellii strain DS40M4

46.691

98.415

0.46
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.563

100

0.396
  pilF Thermus thermophilus HB27

36.888

100

0.371

Multiple sequence alignment