Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   VN23_RS17660 Genome accession   NZ_CP014222
Coordinates   3912426..3912839 (-) Length   137 a.a.
NCBI ID   WP_046353749.1    Uniprot ID   -
Organism   Janthinobacterium sp. B9-8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3907426..3917839
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VN23_RS17635 (VN23_17635) cysN 3907449..3908885 (+) 1437 WP_046353744.1 sulfate adenylyltransferase subunit CysN -
  VN23_RS17640 (VN23_17640) - 3909043..3909468 (-) 426 WP_046353745.1 pilin -
  VN23_RS17645 (VN23_17645) - 3909704..3910153 (-) 450 WP_269465457.1 pilin -
  VN23_RS17650 (VN23_17650) - 3910276..3911889 (-) 1614 WP_046353747.1 tetratricopeptide repeat protein -
  VN23_RS17655 (VN23_17655) pilA2 3911955..3912368 (-) 414 WP_046353748.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  VN23_RS17660 (VN23_17660) pilA2 3912426..3912839 (-) 414 WP_046353749.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  VN23_RS17670 (VN23_17670) - 3913358..3913954 (-) 597 WP_046353750.1 Maf family protein -
  VN23_RS17675 (VN23_17675) rlmH 3914070..3914540 (-) 471 WP_046353751.1 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH -
  VN23_RS17680 (VN23_17680) - 3914537..3916087 (-) 1551 WP_197432948.1 EAL domain-containing protein -
  VN23_RS17685 (VN23_17685) - 3916155..3916841 (+) 687 WP_046353752.1 TIGR01621 family pseudouridine synthase -
  VN23_RS17690 (VN23_17690) - 3916935..3917702 (-) 768 WP_046353753.1 LytTR family DNA-binding domain-containing protein -

Sequence

Protein


Download         Length: 137 a.a.        Molecular weight: 14127.34 Da        Isoelectric Point: 8.0987

>NTDB_id=169558 VN23_RS17660 WP_046353749.1 3912426..3912839(-) (pilA2) [Janthinobacterium sp. B9-8]
MKNLQKGFTLIELMIVVAIIGILAAVAIPSYQDYTKKAKFSEVLSISDSYKQAVAMCQSDLNTFTGCNAGTQGIPATPTV
ATTGLATGGTVTNGVISFTGTAAAGAYTFILTPALNAEGTAITWTQSGTCLAVKYCK

Nucleotide


Download         Length: 414 bp        

>NTDB_id=169558 VN23_RS17660 WP_046353749.1 3912426..3912839(-) (pilA2) [Janthinobacterium sp. B9-8]
ATGAAAAACTTACAAAAAGGTTTTACCCTGATCGAACTGATGATTGTGGTCGCCATTATCGGTATCCTGGCCGCCGTTGC
GATTCCGTCTTATCAGGACTATACGAAGAAAGCGAAGTTCTCTGAAGTGCTGAGTATTTCGGATTCATACAAACAAGCTG
TGGCGATGTGCCAGTCAGATTTAAATACGTTTACGGGATGTAATGCAGGTACTCAAGGTATTCCGGCTACCCCAACTGTA
GCTACAACAGGCTTAGCAACAGGGGGTACGGTAACAAATGGCGTTATTTCCTTTACAGGTACAGCGGCAGCAGGTGCATA
TACCTTTATTTTAACTCCTGCTTTAAATGCAGAAGGGACTGCAATTACGTGGACACAATCTGGTACATGCTTAGCTGTTA
AGTACTGCAAGTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

52.632

97.08

0.511
  pilA2 Legionella pneumophila strain ERS1305867

51.145

95.62

0.489
  pilA Ralstonia pseudosolanacearum GMI1000

49.6

91.241

0.453
  pilA Haemophilus influenzae 86-028NP

48

91.241

0.438
  comP Acinetobacter baylyi ADP1

43.182

96.35

0.416
  pilA Haemophilus influenzae Rd KW20

45.6

91.241

0.416
  pilA Glaesserella parasuis strain SC1401

40.769

94.891

0.387
  pilV Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.594

97.08

0.365

Multiple sequence alignment