Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   VN23_RS17655 Genome accession   NZ_CP014222
Coordinates   3911955..3912368 (-) Length   137 a.a.
NCBI ID   WP_046353748.1    Uniprot ID   A0A0X8GSE7
Organism   Janthinobacterium sp. B9-8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3906955..3917368
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VN23_RS17635 (VN23_17635) cysN 3907449..3908885 (+) 1437 WP_046353744.1 sulfate adenylyltransferase subunit CysN -
  VN23_RS17640 (VN23_17640) - 3909043..3909468 (-) 426 WP_046353745.1 pilin -
  VN23_RS17645 (VN23_17645) - 3909704..3910153 (-) 450 WP_269465457.1 pilin -
  VN23_RS17650 (VN23_17650) - 3910276..3911889 (-) 1614 WP_046353747.1 tetratricopeptide repeat protein -
  VN23_RS17655 (VN23_17655) pilA2 3911955..3912368 (-) 414 WP_046353748.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  VN23_RS17660 (VN23_17660) pilA2 3912426..3912839 (-) 414 WP_046353749.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  VN23_RS17670 (VN23_17670) - 3913358..3913954 (-) 597 WP_046353750.1 Maf family protein -
  VN23_RS17675 (VN23_17675) rlmH 3914070..3914540 (-) 471 WP_046353751.1 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH -
  VN23_RS17680 (VN23_17680) - 3914537..3916087 (-) 1551 WP_197432948.1 EAL domain-containing protein -
  VN23_RS17685 (VN23_17685) - 3916155..3916841 (+) 687 WP_046353752.1 TIGR01621 family pseudouridine synthase -

Sequence

Protein


Download         Length: 137 a.a.        Molecular weight: 14136.33 Da        Isoelectric Point: 8.1053

>NTDB_id=169557 VN23_RS17655 WP_046353748.1 3911955..3912368(-) (pilA2) [Janthinobacterium sp. B9-8]
MQYIQKGFTLIELMIVVAIIGILAAVAIPSYQDYTKKAKFSEVLSISDSYKQAVALCHADLNTFTGCNAGTQGIPATPTV
ATTGLATGGTVTNGVITFTGTAAAGGYTFVLTPTLNAQGTAITWAQTGTCLGVKYCK

Nucleotide


Download         Length: 414 bp        

>NTDB_id=169557 VN23_RS17655 WP_046353748.1 3911955..3912368(-) (pilA2) [Janthinobacterium sp. B9-8]
ATGCAATACATCCAAAAAGGTTTTACATTAATTGAATTGATGATTGTTGTGGCAATTATAGGAATTTTAGCTGCCGTAGC
AATTCCCTCTTATCAAGATTATACAAAAAAAGCGAAATTTTCTGAAGTTCTCAGTATTTCTGATTCATATAAACAAGCGG
TTGCATTGTGCCATGCTGACTTGAATACATTTACAGGTTGTAATGCAGGTACTCAAGGTATTCCAGCAACTCCCACTGTA
GCTACAACAGGCTTAGCAACAGGGGGAACTGTAACAAATGGCGTGATTACATTTACTGGTACAGCAGCAGCGGGTGGCTA
CACTTTTGTTTTGACACCAACTTTAAATGCACAGGGGACAGCTATTACTTGGGCACAAACAGGGACGTGTTTGGGTGTTA
AATATTGTAAGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-27)

Pilin (bacterial filament) (Pilin)

(37-128)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0X8GSE7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

55.639

97.08

0.54
  pilA2 Legionella pneumophila strain ERS1305867

52.672

95.62

0.504
  pilA Haemophilus influenzae 86-028NP

49.618

95.62

0.474
  pilA Haemophilus influenzae Rd KW20

46.212

96.35

0.445
  pilA Ralstonia pseudosolanacearum GMI1000

50.413

88.321

0.445
  comP Acinetobacter baylyi ADP1

45.736

94.161

0.431
  pilA Glaesserella parasuis strain SC1401

42.188

93.431

0.394
  pilA Vibrio campbellii strain DS40M4

62.5

58.394

0.365

Multiple sequence alignment