Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AL536_RS44810 Genome accession   NZ_CP014035
Coordinates   3054496..3056232 (+) Length   578 a.a.
NCBI ID   WP_061057156.1    Uniprot ID   A0AAX2LN48
Organism   Vibrio fluvialis strain ATCC 33809     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3049496..3061232
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL536_RS44790 (AL536_21810) pilM 3051802..3052806 (+) 1005 WP_061057154.1 type IV pilus assembly protein PilM Machinery gene
  AL536_RS44795 (AL536_21815) pilN 3052796..3053386 (+) 591 WP_061057155.1 PilN domain-containing protein Machinery gene
  AL536_RS44800 (AL536_21820) pilO 3053379..3053969 (+) 591 WP_020429291.1 type 4a pilus biogenesis protein PilO Machinery gene
  AL536_RS44805 (AL536_21825) pilP 3053959..3054471 (+) 513 WP_020429290.1 pilus assembly protein PilP Machinery gene
  AL536_RS44810 (AL536_21830) pilQ 3054496..3056232 (+) 1737 WP_061057156.1 type IV pilus secretin PilQ Machinery gene
  AL536_RS44815 (AL536_21835) aroK 3056420..3056938 (+) 519 WP_004728569.1 shikimate kinase AroK -
  AL536_RS44820 (AL536_21840) aroB 3056966..3058051 (+) 1086 WP_024374091.1 3-dehydroquinate synthase -
  AL536_RS44825 (AL536_21845) - 3058097..3059605 (+) 1509 WP_061057157.1 AAA family ATPase -
  AL536_RS44830 (AL536_21850) - 3059700..3060530 (+) 831 WP_020329450.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62453.38 Da        Isoelectric Point: 4.5714

>NTDB_id=167716 AL536_RS44810 WP_061057156.1 3054496..3056232(+) (pilQ) [Vibrio fluvialis strain ATCC 33809]
MIKGLNASTAWGIRCLFVGACLLLSAATLAQEAAANQLKNIDFRINKDKAAVLIVELASASAVVDVQKTEQGLNIELLKT
DVSDDKLYLLDVRDFATAVENIEVFRQQPSTLLVATVQGEFNYDYSLKGNYLEVVIRKLKAEEKAKPKSVLEKEGKRISI
NFQDIPVRNVLQLIADYNEFNLVVSDSVRGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKEELDLREKQQLE
KARMAEELGDLQSDILIINFAKASDIAQMIGGDGAVNMLSERGSISIDERTNALLIRELPENIAVIRDIVASLDIPVKQV
QIEARIVTVNEGNLDELGVRWGFSSTNGSTTTGGSIESNLGAIGLYDGGNNSGGSGDGVAIDDFLNVNLAATSANASSIA
FQVAKLGADTLLDLELSAMQRESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGATSVAFKKAVLSLKVTPQITPD
NRLVLDLSVTQDRPGEVVKTGTGEAVAINTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPVLGALFRRSYE
QMGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=167716 AL536_RS44810 WP_061057156.1 3054496..3056232(+) (pilQ) [Vibrio fluvialis strain ATCC 33809]
ATGATTAAAGGACTAAATGCCTCCACGGCGTGGGGAATACGTTGTCTGTTTGTGGGCGCATGCCTGCTGTTATCTGCCGC
CACACTTGCTCAGGAGGCGGCGGCCAATCAGCTGAAGAATATCGATTTTCGCATCAACAAAGACAAAGCGGCGGTACTGA
TTGTCGAACTGGCTTCCGCCTCGGCCGTGGTGGATGTGCAGAAAACCGAGCAAGGCCTGAATATCGAACTGCTGAAAACC
GATGTCAGTGACGACAAACTCTACCTGCTCGATGTGCGCGACTTTGCCACCGCAGTGGAAAACATCGAAGTGTTCCGCCA
GCAGCCCAGCACCTTGTTGGTCGCAACGGTGCAGGGGGAGTTCAATTATGATTACAGCCTGAAGGGTAACTATCTGGAAG
TGGTGATCCGCAAGCTAAAAGCCGAGGAGAAAGCCAAACCAAAAAGCGTACTAGAAAAAGAAGGCAAGCGGATTTCGATT
AACTTTCAGGATATTCCGGTGCGCAATGTGCTGCAGTTGATCGCAGACTACAATGAGTTCAATCTGGTGGTTTCCGATTC
AGTGCGCGGCAACCTGACGCTGCGCCTTGATGGCGTGCCCTGGCAGCAGGTGCTGGACATTATTCTGCAAGTCAAAGGTC
TGGATAAGCGCGTCGACGGCAATGTGATTCTGGTGGCGCCCAAAGAAGAGCTGGATCTGCGTGAAAAGCAGCAGTTGGAA
AAAGCCCGCATGGCGGAAGAACTCGGCGATCTGCAATCGGATATTCTCATCATCAACTTTGCCAAAGCGTCTGACATTGC
GCAGATGATTGGCGGCGATGGGGCGGTCAATATGTTGTCGGAGCGTGGCTCGATCAGCATTGATGAACGCACCAATGCGC
TGCTGATTCGTGAACTGCCGGAGAACATTGCTGTGATTCGCGATATCGTGGCGTCGCTCGATATTCCGGTCAAGCAAGTA
CAGATTGAAGCGCGTATTGTGACGGTCAATGAAGGTAACTTGGATGAACTGGGTGTGCGCTGGGGTTTCAGCTCCACCAA
TGGCAGCACGACCACGGGTGGCTCGATTGAAAGCAATCTGGGGGCAATTGGTTTGTACGATGGCGGTAATAATAGTGGCG
GTTCTGGTGATGGCGTTGCGATTGATGATTTCCTCAACGTTAACCTCGCAGCCACCTCAGCCAATGCGTCCAGCATTGCG
TTTCAGGTCGCCAAGCTTGGCGCAGATACCTTGCTCGATCTTGAACTGTCAGCCATGCAACGCGAGTCAAAAGCGGAAAT
CATCTCCAGTCCGAGACTGATCACGACCAACAAGCAGCCGGCGTATATTGAGCAGGGTACTGAGATTCCGTACTTGGAAT
CCTCATCCAGTGGCGCCACCAGTGTGGCATTCAAGAAGGCAGTACTCAGCCTCAAAGTGACGCCACAAATCACCCCCGAT
AACCGTCTGGTGCTGGATTTGAGCGTTACTCAGGATCGGCCGGGTGAAGTGGTGAAAACCGGTACTGGCGAGGCGGTGGC
GATCAACACGCAGCGCATTGGTACACAGGTGCTGGTCAACAACGGTGAAACCGTGGTTCTGGGCGGCATTTTTCAGCACA
GTATTACGAATTCAGTCGACAAGGTGCCGCTGCTGGGTGACTTGCCTGTGTTGGGCGCGCTGTTCCGTCGCAGCTACGAA
CAAATGGGCAAAAGTGAGCTGCTGATCTTCGTAACACCGAAAGTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(418-576)

Bacterial type II/III secretion system short domain (Secretin_N)

(258-319)

Secretin and TonB N terminus short domain (STN)

(180-228)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

81.52

100

0.817
  pilQ Vibrio cholerae strain A1552

81.52

100

0.817
  pilQ Vibrio campbellii strain DS40M4

79.053

94.983

0.751
  pilQ Legionella pneumophila strain Lp02

42.545

95.156

0.405
  pilQ Legionella pneumophila strain ERS1305867

42.545

95.156

0.405
  pilQ Pseudomonas aeruginosa PAK

40.721

96.021

0.391
  comQ Acinetobacter baylyi ADP1

37.973

100

0.382

Multiple sequence alignment