Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   AT746_RS16135 Genome accession   NZ_CP013650
Coordinates   3572010..3573227 (+) Length   405 a.a.
NCBI ID   WP_062482367.1    Uniprot ID   A0A0U2ZAV3
Organism   Lacimicrobium alkaliphilum strain YelD216     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3567010..3578227
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AT746_RS16105 (AT746_16090) pdhR 3567392..3568153 (-) 762 WP_062482352.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  AT746_RS16110 (AT746_16095) ampE 3568395..3569219 (-) 825 WP_062482355.1 beta-lactamase regulator AmpE -
  AT746_RS16115 (AT746_16100) ampD 3569216..3569764 (-) 549 WP_062482358.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AT746_RS16120 (AT746_16105) - 3569836..3570369 (+) 534 WP_231730956.1 retropepsin-like aspartic protease family protein -
  AT746_RS16125 (AT746_16110) nadC 3570454..3571308 (+) 855 WP_062482361.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AT746_RS16130 (AT746_16115) - 3571506..3571919 (+) 414 WP_062482364.1 pilin -
  AT746_RS16135 (AT746_16120) pilC 3572010..3573227 (+) 1218 WP_062482367.1 type II secretion system F family protein Machinery gene
  AT746_RS16140 (AT746_16125) - 3573275..3573859 (+) 585 WP_062482370.1 hypothetical protein -
  AT746_RS16145 (AT746_16130) pilD 3573882..3574754 (+) 873 WP_062482373.1 prepilin peptidase Machinery gene
  AT746_RS16150 (AT746_16135) coaE 3574751..3575365 (+) 615 WP_062482376.1 dephospho-CoA kinase -
  AT746_RS16155 (AT746_16140) zapD 3575420..3576172 (+) 753 WP_062482379.1 cell division protein ZapD -
  AT746_RS16160 (AT746_16145) yacG 3576198..3576428 (+) 231 WP_062482382.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44022.00 Da        Isoelectric Point: 9.9745

>NTDB_id=163376 AT746_RS16135 WP_062482367.1 3572010..3573227(+) (pilC) [Lacimicrobium alkaliphilum strain YelD216]
MAKAKSLDIFVWQGKNRKGQQVKGEISAKSMTEARNLLRRQGVSATKVKKFSKPLFGKGSAKINAEDICIVSRQIATMLG
AGVTLIQTIDMIAEGHSKASMRKMLTDIGNEVKAGNPLSYALRKHPDHFEDLYCDLVETGEQSGALETIYDRIATYKEKA
EALKSKIKKAMFYPIAVIVVAFIVTTVLLIFVVPQFEEIFSSFGAELPAFTQFVLGISRFVQNNGLYIAAAMFVGAILFA
RAYKKSRPLRDKIDKKILKVPVIGEILKKASIARFTRTLATTFAAGVPLIGALESAAGAAGNAVFRDAILHIRKEVAGGM
PMNVAMRATAVFPDMVTQMIAIGEESGAVDEMLSKIATIYEREVDDMVDGLTSLLEPMIMAVLGVVIGGLIVAMYLPIFQ
MGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=163376 AT746_RS16135 WP_062482367.1 3572010..3573227(+) (pilC) [Lacimicrobium alkaliphilum strain YelD216]
ATGGCAAAAGCAAAATCGCTGGATATTTTTGTCTGGCAGGGTAAAAACCGTAAAGGTCAGCAGGTAAAAGGCGAGATCAG
CGCCAAGTCTATGACCGAGGCCCGCAATCTGCTAAGACGACAGGGGGTTTCCGCCACTAAGGTAAAAAAATTTTCCAAAC
CCTTATTTGGTAAAGGTTCGGCCAAAATCAATGCCGAAGATATCTGTATCGTTTCCCGTCAGATTGCCACCATGCTCGGC
GCCGGTGTCACTCTGATCCAGACCATAGATATGATCGCCGAAGGCCACTCAAAGGCCAGTATGCGCAAGATGCTGACCGA
TATCGGCAATGAGGTCAAAGCCGGGAATCCGCTCTCTTATGCCCTGCGCAAACACCCGGATCATTTTGAGGATCTCTACT
GCGACCTGGTGGAAACCGGTGAGCAGTCCGGTGCCCTGGAAACCATTTATGATCGTATCGCCACCTATAAAGAAAAAGCC
GAGGCATTAAAATCCAAGATTAAAAAGGCCATGTTCTACCCTATCGCAGTCATTGTGGTGGCCTTTATCGTCACCACCGT
ACTATTGATATTTGTGGTACCCCAATTCGAGGAGATCTTCAGCAGCTTTGGCGCCGAGCTGCCGGCCTTTACTCAGTTTG
TATTAGGTATCTCCCGTTTTGTACAGAACAACGGGTTGTATATTGCCGCCGCCATGTTTGTGGGCGCCATACTCTTTGCC
CGGGCCTATAAAAAATCGCGGCCACTGAGAGATAAGATTGACAAGAAAATCCTTAAGGTTCCGGTTATCGGCGAGATCCT
GAAAAAAGCCTCCATTGCCCGCTTTACCCGCACCCTGGCTACCACTTTTGCTGCCGGTGTGCCTTTGATTGGTGCGCTGG
AGTCCGCCGCCGGTGCGGCCGGTAATGCGGTATTCAGAGATGCTATATTGCATATCCGCAAGGAAGTGGCCGGTGGTATG
CCGATGAACGTGGCCATGCGTGCCACTGCGGTATTCCCGGATATGGTGACGCAGATGATTGCCATCGGCGAAGAATCCGG
CGCCGTCGACGAGATGCTAAGCAAAATTGCCACTATCTATGAGCGTGAAGTGGACGATATGGTAGATGGCCTCACCAGCC
TGCTCGAACCCATGATTATGGCAGTGCTCGGGGTGGTTATCGGTGGCCTGATCGTGGCCATGTACCTGCCTATCTTCCAG
ATGGGTAATGTGGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0U2ZAV3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

57.921

99.753

0.578
  pilC Acinetobacter baumannii D1279779

52.228

99.753

0.521
  pilC Acinetobacter baylyi ADP1

50.493

100

0.506
  pilC Legionella pneumophila strain ERS1305867

50.251

98.272

0.494
  pilC Vibrio cholerae strain A1552

46.366

98.519

0.457
  pilC Vibrio campbellii strain DS40M4

44.417

99.506

0.442
  pilG Neisseria meningitidis 44/76-A

43.35

100

0.435
  pilG Neisseria gonorrhoeae MS11

43.103

100

0.432
  pilC Thermus thermophilus HB27

37.129

99.753

0.37

Multiple sequence alignment