Detailed information NTG0163377

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AT746_RS16145
• Genome accession: NZ_CP013650
• Coordinates: 3573882..3574754 (+)
• Length: 290 a.a.
• NCBI ID: WP_062482373.1
• Uniprot ID: A0A0U2JJI5
• Organism: Lacimicrobium alkaliphilum strain YelD216
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3568882..3579754

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AT746_RS16115 (AT746_16100)	ampD	3569216..3569764 (-)	549	WP_062482358.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
AT746_RS16120 (AT746_16105)	-	3569836..3570369 (+)	534	WP_231730956.1	retropepsin-like aspartic protease family protein	-
AT746_RS16125 (AT746_16110)	nadC	3570454..3571308 (+)	855	WP_062482361.1	carboxylating nicotinate-nucleotide diphosphorylase	-
AT746_RS16130 (AT746_16115)	-	3571506..3571919 (+)	414	WP_062482364.1	pilin	-
AT746_RS16135 (AT746_16120)	pilC	3572010..3573227 (+)	1218	WP_062482367.1	type II secretion system F family protein	Machinery gene
AT746_RS16140 (AT746_16125)	-	3573275..3573859 (+)	585	WP_062482370.1	hypothetical protein	-
AT746_RS16145 (AT746_16130)	pilD	3573882..3574754 (+)	873	WP_062482373.1	prepilin peptidase	Machinery gene
AT746_RS16150 (AT746_16135)	coaE	3574751..3575365 (+)	615	WP_062482376.1	dephospho-CoA kinase	-
AT746_RS16155 (AT746_16140)	zapD	3575420..3576172 (+)	753	WP_062482379.1	cell division protein ZapD	-
AT746_RS16160 (AT746_16145)	yacG	3576198..3576428 (+)	231	WP_062482382.1	DNA gyrase inhibitor YacG	-
AT746_RS16165 (AT746_16150)	dxs	3577414..3579282 (-)	1869	WP_062482384.1	1-deoxy-D-xylulose-5-phosphate synthase	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32561.76 Da
• Isoelectric Point: 6.8443

>NTDB_id=163377 AT746_RS16145 WP_062482373.1 3573882..3574754(+) (pilD) [Lacimicrobium alkaliphilum strain YelD216]
MNDMMLLLGDSPAVLIGFTLVLGLMVGSFLNVVIHRLPIMMERSWKQEYQSYFDPEKSPEQSRFNLIKPDSQCPVCQHKI
RAWENIPLLSWIWLKGKCSQCKTPISARYPLVELLTGLISGLLAWHFGFGWPLLAALIFSWLLIAMTFIDLDRMLLPDQL
TLPLLWLGLLLSTQHVFVSPVEAIIGAAAGYLSLWSVYWGFKLLTGKEGMGYGDFKLLAALGAWVGWQYLPMIILLSSFV
GAILGLILLSVQGKDKGQAIPFGPYLAVAGWVTFLYGEQLSDIYWSWMLA

Nucleotide

• Length: 873 bp

>NTDB_id=163377 AT746_RS16145 WP_062482373.1 3573882..3574754(+) (pilD) [Lacimicrobium alkaliphilum strain YelD216]
ATGAACGATATGATGCTGTTGCTGGGCGATTCTCCCGCTGTTCTGATCGGTTTCACCCTGGTGCTGGGGTTGATGGTGGG
CAGTTTTTTAAATGTGGTGATCCACAGACTGCCGATTATGATGGAGCGCAGCTGGAAGCAGGAATATCAAAGCTATTTTG
ATCCTGAAAAAAGTCCTGAACAAAGCCGTTTTAACCTGATTAAACCGGATTCACAATGTCCGGTATGCCAGCATAAGATA
AGGGCCTGGGAAAATATCCCGCTGCTGAGCTGGATATGGCTTAAGGGTAAGTGCAGCCAGTGTAAAACGCCCATTTCTGC
CCGTTATCCCCTGGTGGAATTACTGACTGGCCTTATCAGCGGCTTACTGGCATGGCATTTTGGCTTTGGCTGGCCTTTGC
TGGCAGCACTGATATTCAGCTGGCTGCTTATAGCCATGACCTTTATCGATCTGGATCGCATGTTATTGCCGGATCAACTG
ACCCTGCCTCTGCTTTGGCTGGGGCTGCTATTAAGTACTCAGCATGTTTTTGTGTCCCCCGTTGAGGCGATTATCGGTGC
TGCCGCCGGTTACCTGAGTCTGTGGTCGGTTTACTGGGGGTTTAAATTACTTACCGGTAAGGAGGGCATGGGCTATGGCG
ATTTTAAATTACTGGCGGCGCTGGGTGCCTGGGTGGGCTGGCAATATCTGCCAATGATTATTCTGTTGTCCTCTTTTGTC
GGCGCGATTCTGGGTCTCATCCTGTTATCCGTGCAGGGCAAAGACAAAGGTCAGGCCATCCCCTTTGGTCCTTATCTGGC
CGTGGCCGGATGGGTGACTTTCCTGTATGGAGAACAACTAAGCGACATATACTGGTCCTGGATGCTGGCATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (138-246)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-127)

3D structure

• AlphaFold DB: A0A0U2JJI5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	60.886	93.448	0.569
pilD	Vibrio campbellii strain DS40M4	56.537	97.586	0.552
pilD	Acinetobacter baumannii D1279779	53.48	94.138	0.503
pilD	Acinetobacter nosocomialis M2	53.114	94.138	0.5
pilD	Neisseria gonorrhoeae MS11	51.341	90	0.462