Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACA40_RS04015 Genome accession   NZ_CP013183
Coordinates   902456..903673 (-) Length   405 a.a.
NCBI ID   WP_057405957.1    Uniprot ID   A0AB74A3R3
Organism   Pseudomonas syringae pv. lapsa strain ATCC 10859     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 897456..908673
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACA40_RS03985 (ACA40_03985) - 897828..898295 (+) 468 WP_057405993.1 hypothetical protein -
  ACA40_RS03990 (ACA40_03990) - 898586..900058 (+) 1473 WP_057405960.1 tetratricopeptide repeat protein -
  ACA40_RS03995 (ACA40_03995) - 900165..900623 (+) 459 WP_010415567.1 hypothetical protein -
  ACA40_RS04000 (ACA40_04000) yacG 900756..900965 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  ACA40_RS04005 (ACA40_04005) coaE 900962..901585 (-) 624 WP_057405959.1 dephospho-CoA kinase -
  ACA40_RS04010 (ACA40_04010) pilD 901582..902454 (-) 873 WP_057405958.1 prepilin peptidase Machinery gene
  ACA40_RS04015 (ACA40_04015) pilC 902456..903673 (-) 1218 WP_057405957.1 type II secretion system F family protein Machinery gene
  ACA40_RS04020 (ACA40_04020) pilB 903676..905370 (-) 1695 WP_057405956.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACA40_RS25745 - 905597..906031 (+) 435 WP_080391914.1 pilin -
  ACA40_RS04030 (ACA40_04030) - 906805..907392 (+) 588 WP_161806257.1 DUF433 domain-containing protein -
  ACA40_RS04035 (ACA40_04035) - 907440..907985 (-) 546 WP_010415546.1 DUF1523 family protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43993.03 Da        Isoelectric Point: 10.2885

>NTDB_id=160432 ACA40_RS04015 WP_057405957.1 902456..903673(-) (pilC) [Pseudomonas syringae pv. lapsa strain ATCC 10859]
MASRAVKVIVYTWEGVDKKGTKASGELSGHNMALVKAQLRKQGINPTKVRKKSASIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIISEGAENPNMRTLVSSLKQEVSAGNSFATALRQKPEYFDDLFCNLVDAGEQAGALESLLDRVASYKEKT
EKLKAKIKKAMTYPIAVLIVAIIVSGILLIKVVPQFQSVFAGFGADLPAFTLMVIGLSNVVQKWWLIIVGLLFAGFFLFK
RAYKKSQKFRDGLDRLLLKAPLIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVVFKNAVTKVKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDTMLDKVATYYEDEVDNMVDNLTSLMEPMIMAFLGVIVGGLVIAMYLPIFK
LGSIV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=160432 ACA40_RS04015 WP_057405957.1 902456..903673(-) (pilC) [Pseudomonas syringae pv. lapsa strain ATCC 10859]
ATGGCCAGCAGGGCAGTCAAAGTCATCGTTTACACGTGGGAAGGTGTGGACAAGAAGGGCACTAAAGCCAGTGGCGAACT
GAGCGGCCATAATATGGCACTCGTAAAAGCCCAATTGCGCAAGCAAGGCATCAACCCCACCAAGGTGCGCAAGAAGTCCG
CATCGATCTTCGGCAAAGGCAAGAAGATCAAGCCATTGGACATCGCGTTTTTTTCTCGGCAGATGGCAACGATGATGAAG
GCTGGCGTGCCGTTGCTGCAGTCGTTTGACATCATCAGCGAGGGCGCTGAAAACCCAAACATGCGTACACTTGTGAGCTC
GTTAAAGCAGGAAGTGTCGGCAGGCAACAGCTTTGCGACGGCGTTGCGACAGAAACCGGAATATTTCGATGACCTGTTTT
GCAATCTGGTAGATGCCGGCGAACAAGCGGGGGCACTTGAAAGTCTTCTGGACCGGGTAGCTAGCTACAAGGAGAAGACC
GAAAAGCTAAAAGCCAAAATTAAAAAAGCCATGACATACCCAATCGCCGTGTTGATCGTAGCGATTATTGTGTCAGGTAT
TTTGTTGATCAAGGTGGTACCGCAGTTCCAATCCGTATTTGCTGGCTTTGGTGCTGACTTACCTGCGTTTACTCTGATGG
TTATTGGTCTATCAAACGTTGTTCAGAAATGGTGGCTGATAATTGTCGGTTTATTGTTTGCAGGGTTTTTTTTATTTAAG
CGGGCCTACAAAAAATCTCAAAAATTTCGCGACGGCCTTGATCGTCTTTTGCTAAAAGCCCCCCTCATCGGGCCTCTTAT
TTTCAAATCTTCGGTAGCTCGCTATGCGCGCACACTTGCTACGACTTTTGCCGCTGGCGTCCCGCTTGTGGAGGCTTTGG
ACTCAGTCGCCGGAGCGACTGGCAATGTAGTATTTAAAAATGCAGTCACCAAGGTAAAACAGGACGTCTCCACGGGCATG
CAACTGAATTTTTCGATGCGTTCAACAGGAGTATTTCCGAGCCTTGCGATACAGATGACCGCGATTGGCGAAGAGTCCGG
CGCCTTGGATACGATGCTGGACAAGGTCGCTACCTACTATGAGGACGAAGTCGATAACATGGTCGACAATCTGACCAGCC
TTATGGAACCGATGATCATGGCCTTTTTGGGAGTGATCGTCGGAGGCCTTGTCATTGCCATGTATCTCCCCATCTTCAAA
CTTGGAAGCATTGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.333

100

0.733
  pilC Acinetobacter baylyi ADP1

60.539

100

0.61
  pilC Acinetobacter baumannii D1279779

61.209

98.025

0.6
  pilC Legionella pneumophila strain ERS1305867

52.217

100

0.523
  pilC Vibrio campbellii strain DS40M4

42.75

98.765

0.422
  pilG Neisseria gonorrhoeae MS11

42.714

98.272

0.42
  pilG Neisseria meningitidis 44/76-A

42.714

98.272

0.42
  pilC Vibrio cholerae strain A1552

41.728

100

0.417
  pilC Thermus thermophilus HB27

38.404

99.012

0.38

Multiple sequence alignment