Detailed information NTG0160431

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ACA40_RS04010
• Genome accession: NZ_CP013183
• Coordinates: 901582..902454 (-)
• Length: 290 a.a.
• NCBI ID: WP_057405958.1
• Uniprot ID: A0AB74A415
• Organism: Pseudomonas syringae pv. lapsa strain ATCC 10859
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 896582..907454

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACA40_RS03980 (ACA40_03980)	-	897071..897700 (-)	630	WP_003345130.1	DUF1780 domain-containing protein	-
ACA40_RS03985 (ACA40_03985)	-	897828..898295 (+)	468	WP_057405993.1	hypothetical protein	-
ACA40_RS03990 (ACA40_03990)	-	898586..900058 (+)	1473	WP_057405960.1	tetratricopeptide repeat protein	-
ACA40_RS03995 (ACA40_03995)	-	900165..900623 (+)	459	WP_010415567.1	hypothetical protein	-
ACA40_RS04000 (ACA40_04000)	yacG	900756..900965 (-)	210	WP_002552020.1	DNA gyrase inhibitor YacG	-
ACA40_RS04005 (ACA40_04005)	coaE	900962..901585 (-)	624	WP_057405959.1	dephospho-CoA kinase	-
ACA40_RS04010 (ACA40_04010)	pilD	901582..902454 (-)	873	WP_057405958.1	prepilin peptidase	Machinery gene
ACA40_RS04015 (ACA40_04015)	pilC	902456..903673 (-)	1218	WP_057405957.1	type II secretion system F family protein	Machinery gene
ACA40_RS04020 (ACA40_04020)	pilB	903676..905370 (-)	1695	WP_057405956.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ACA40_RS25745	-	905597..906031 (+)	435	WP_080391914.1	pilin	-
ACA40_RS04030 (ACA40_04030)	-	906805..907392 (+)	588	WP_161806257.1	DUF433 domain-containing protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32039.27 Da
• Isoelectric Point: 8.3734

>NTDB_id=160431 ACA40_RS04010 WP_057405958.1 901582..902454(-) (pilD) [Pseudomonas syringae pv. lapsa strain ATCC 10859]
MPLLDLLASSPLAFVITCCILGLIIGSFLNVVVYRLPIMMERDWKAQSRELLGLPADPDQPVFNLNRPRSSCPHCAHKIR
PWENLPVISYLLLRGKCSQCKAPISKRYPLVELTCAMLSAYVAWHFGFGWQAAAMLVLSWGLLAMSLIDADHQLLPDSLV
LPLLWLGLIVNAFGLFTSLNDALWGAVAGYLALWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AVLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=160431 ACA40_RS04010 WP_057405958.1 901582..902454(-) (pilD) [Pseudomonas syringae pv. lapsa strain ATCC 10859]
ATGCCCCTCCTAGACCTCCTGGCAAGCTCCCCCCTGGCCTTCGTCATCACCTGCTGCATCCTCGGCCTGATCATCGGAAG
CTTCCTCAACGTAGTCGTCTATCGCCTGCCAATAATGATGGAGCGCGACTGGAAAGCCCAATCCCGTGAACTGCTCGGCC
TGCCCGCCGACCCCGATCAACCCGTCTTCAACCTGAATCGTCCCCGCTCCAGTTGCCCGCATTGCGCACACAAAATCCGC
CCATGGGAAAACCTGCCGGTTATCAGCTATCTGCTGCTGCGCGGTAAATGTTCGCAGTGCAAAGCGCCGATCAGCAAACG
CTACCCGCTGGTCGAGCTGACCTGCGCAATGTTGTCGGCCTACGTAGCCTGGCATTTCGGCTTTGGCTGGCAGGCGGCGG
CAATGCTGGTACTGAGCTGGGGGCTGCTGGCGATGAGTCTGATCGATGCTGATCACCAGCTGTTACCTGATTCTCTGGTG
CTGCCACTGCTGTGGCTGGGCCTGATCGTCAACGCCTTCGGGCTGTTCACCTCGCTGAATGATGCGCTGTGGGGTGCAGT
GGCCGGGTATCTGGCGCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTTACAGGCAAGGAAGGCATGGGTTATGGCGACT
TCAAGTTGCTGGCCATGCTTGGCGCGTGGGGCGGCTGGCAGATTTTGCCGCTGACCATCCTGTTATCGTCGCTGGTGGGC
GCGGTGCTTGGGGTGATCATGATGCGTGTGCGCAGGGTCGAAAGCGGTACGCCGATCCCCTTTGGTCCTTATCTGGCCAT
TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTACATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.169	97.931	0.521
pilD	Vibrio campbellii strain DS40M4	51.943	97.586	0.507
pilD	Acinetobacter baumannii D1279779	52.347	95.517	0.5
pilD	Acinetobacter nosocomialis M2	51.625	95.517	0.493
pilD	Neisseria gonorrhoeae MS11	51.119	92.414	0.472