Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   D172_RS02315 Genome accession   NZ_CP013138
Coordinates   546326..547555 (-) Length   409 a.a.
NCBI ID   WP_007376053.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. Bsw20308     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 541326..552555
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D172_RS02290 (D172_002290) - 541915..542403 (+) 489 WP_007376048.1 periplasmic heavy metal sensor -
  D172_RS02295 (D172_002295) yacG 542476..542706 (-) 231 WP_007376049.1 DNA gyrase inhibitor YacG -
  D172_RS02300 (D172_002300) pilB 542822..544498 (-) 1677 WP_007376050.1 type IV-A pilus assembly ATPase PilB Machinery gene
  D172_RS02305 (D172_002305) coaE 544777..545424 (-) 648 WP_007376051.1 dephospho-CoA kinase -
  D172_RS02310 (D172_002310) pilD 545426..546310 (-) 885 WP_007376052.1 prepilin peptidase Machinery gene
  D172_RS02315 (D172_002315) pilC 546326..547555 (-) 1230 WP_007376053.1 type II secretion system F family protein Machinery gene
  D172_RS02320 (D172_002320) - 547649..548992 (-) 1344 WP_007376054.1 O-antigen ligase family protein -
  D172_RS02325 (D172_002325) - 549055..549474 (-) 420 WP_007376055.1 pilin -
  D172_RS02330 (D172_002330) nadC 549936..550781 (-) 846 WP_033023262.1 carboxylating nicotinate-nucleotide diphosphorylase -
  D172_RS02335 (D172_002335) ampD 551462..552016 (+) 555 WP_007378721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 44448.50 Da        Isoelectric Point: 9.7590

>NTDB_id=160263 D172_RS02315 WP_007376053.1 546326..547555(-) (pilC) [Pseudoalteromonas sp. Bsw20308]
MAKKQEDKGLDIFQWVGVSARGKRLEGELAGNSIALVKAQLRKQGITPSKVKRKPKPLFGIQSVQKITPKDIALVTRQIA
TMLMAGVPLIQSIEMIASGSTNKSVTKLISAVGDDVKAGQPLSLSLRKYPRYFDDLYCDLVESGEQSGALDKIFDRIAIY
KEKSEALKSKIKKAMFYPISVLVIAGIVTSILLIFVVPQFQDIFAGFGAELPPFTLFVIGISDFMQAYWWMILIAIVAFG
WTFKEAKLRSLKLRDATDRAILKLPVIGMILNKAAVARYARTLSTTFAAGVPLVDALDSAAGASGNAVYRYAILDIKAEV
SSGNQMNWAMRNSKIFPDMVIQMVAIGEESGSLDGMLAKVATIYEQEVDDAVDGLSSLLEPLIMAVLGVLVGGLIVAMYL
PIFQLGSVI

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=160263 D172_RS02315 WP_007376053.1 546326..547555(-) (pilC) [Pseudoalteromonas sp. Bsw20308]
ATGGCAAAAAAACAAGAAGACAAGGGACTCGACATATTTCAGTGGGTCGGTGTTAGTGCTCGAGGAAAACGCCTTGAAGG
TGAACTCGCCGGAAATAGTATCGCGCTAGTAAAAGCACAGCTTCGTAAGCAGGGTATTACCCCCTCAAAAGTTAAACGTA
AGCCGAAACCTTTATTTGGTATTCAGAGTGTTCAAAAAATAACACCTAAAGATATCGCCTTGGTTACTCGCCAAATTGCC
ACTATGCTAATGGCTGGCGTACCCTTAATTCAATCTATTGAGATGATAGCTTCTGGCTCAACAAATAAAAGCGTTACCAA
ACTTATCTCAGCTGTAGGCGATGATGTAAAAGCTGGTCAGCCTTTGTCTTTATCACTTCGAAAATATCCACGTTATTTTG
ATGACTTATATTGTGATTTAGTTGAATCTGGCGAGCAGTCAGGAGCACTTGATAAAATATTTGATCGCATAGCTATTTAT
AAAGAAAAATCAGAAGCGCTTAAATCAAAAATTAAAAAAGCAATGTTTTATCCCATTTCGGTATTAGTTATTGCAGGCAT
TGTAACCTCGATATTATTAATATTTGTAGTACCACAATTTCAGGATATTTTTGCAGGGTTTGGGGCTGAACTTCCTCCAT
TTACATTATTCGTAATTGGTATTTCTGACTTTATGCAGGCTTATTGGTGGATGATTCTGATTGCGATAGTGGCTTTTGGC
TGGACTTTTAAAGAAGCTAAATTAAGAAGCCTAAAGCTCAGGGATGCAACCGACAGAGCCATACTTAAGCTCCCTGTTAT
TGGCATGATTTTAAACAAAGCAGCTGTGGCACGTTATGCGCGAACACTTTCGACCACATTTGCTGCCGGTGTTCCATTAG
TTGATGCACTTGATTCAGCAGCAGGTGCTTCGGGCAATGCAGTGTACCGTTATGCTATTTTAGATATTAAAGCAGAGGTT
AGCTCAGGTAACCAAATGAACTGGGCAATGAGAAACTCAAAAATCTTCCCTGACATGGTAATTCAAATGGTTGCTATCGG
TGAAGAATCTGGCTCGCTCGATGGTATGTTAGCTAAGGTCGCGACTATTTATGAGCAAGAAGTAGACGATGCTGTAGATG
GCTTATCGAGCTTACTAGAGCCATTAATTATGGCTGTATTAGGTGTACTTGTTGGTGGTTTAATTGTTGCTATGTACCTC
CCTATTTTCCAACTCGGTTCTGTTATTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(280-401)

Type II secretion system (T2SS), protein F (T2SSF)

(76-198)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

61.809

97.311

0.601
  pilC Acinetobacter baumannii D1279779

55.416

97.066

0.538
  pilC Acinetobacter baylyi ADP1

54.5

97.8

0.533
  pilC Legionella pneumophila strain ERS1305867

54.66

97.066

0.531
  pilC Vibrio cholerae strain A1552

44.279

98.289

0.435
  pilG Neisseria gonorrhoeae MS11

44.246

95.599

0.423
  pilG Neisseria meningitidis 44/76-A

43.99

95.599

0.421
  pilC Vibrio campbellii strain DS40M4

42

97.8

0.411

Multiple sequence alignment