Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   D172_RS02300 Genome accession   NZ_CP013138
Coordinates   542822..544498 (-) Length   558 a.a.
NCBI ID   WP_007376050.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. Bsw20308     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 537822..549498
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D172_RS02275 (D172_002275) - 538692..539639 (-) 948 WP_007376045.1 alpha/beta fold hydrolase -
  D172_RS02280 (D172_002280) - 539643..541058 (-) 1416 WP_007376046.1 ATP-binding protein -
  D172_RS02285 (D172_002285) - 541036..541725 (-) 690 WP_007376047.1 response regulator -
  D172_RS02290 (D172_002290) - 541915..542403 (+) 489 WP_007376048.1 periplasmic heavy metal sensor -
  D172_RS02295 (D172_002295) yacG 542476..542706 (-) 231 WP_007376049.1 DNA gyrase inhibitor YacG -
  D172_RS02300 (D172_002300) pilB 542822..544498 (-) 1677 WP_007376050.1 type IV-A pilus assembly ATPase PilB Machinery gene
  D172_RS02305 (D172_002305) coaE 544777..545424 (-) 648 WP_007376051.1 dephospho-CoA kinase -
  D172_RS02310 (D172_002310) pilD 545426..546310 (-) 885 WP_007376052.1 prepilin peptidase Machinery gene
  D172_RS02315 (D172_002315) pilC 546326..547555 (-) 1230 WP_007376053.1 type II secretion system F family protein Machinery gene
  D172_RS02320 (D172_002320) - 547649..548992 (-) 1344 WP_007376054.1 O-antigen ligase family protein -
  D172_RS02325 (D172_002325) - 549055..549474 (-) 420 WP_007376055.1 pilin -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62254.69 Da        Isoelectric Point: 5.6339

>NTDB_id=160261 D172_RS02300 WP_007376050.1 542822..544498(-) (pilB) [Pseudoalteromonas sp. Bsw20308]
MNINSPLLRKFITLGRIDADTVKVKQRDFASTAELIAKCGNIDSKDLAEQCIDLFRVPYFDLKDFEPARIPAELVKEKLI
RKHHILPLVQKGRKVYIAASDPTDYGAFENYEFSTGLSCEIVVVDYIELDKKIDQLLDATGSLNLSDDEFKEFADLESEN
TKEATPKDDEKDDAPIIVYINKILMDAIKKGASDLHFEPYEHKYRIRFRIDGILHEVASPPNTLATKLSARIKVMSRLDI
AEKRKPQDGRIKLKITERKSIDFRVSTMPTLWGEKIVMRILDSSSAMLGIDVLGYEPEQKKLYMDALAQPQGMILVTGPT
GSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGINQVQINIKADMTFANALRAFLRQDPDVVMVGEIRDLETAEISIK
AAQTGHLVLSTLHTNSAPETITRLLNMGVPAYNVASSISLIIAQRLARRLCPKCKTPEILPNEELTRQGFSEQQISEMTL
YAPKGCDNCTDGYKGRVGIYEVMQITPEIAQIIMRGGNSLEIAEVSLKAGFNNLRLSGIRKAADGMTSLAEINRVTSF

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=160261 D172_RS02300 WP_007376050.1 542822..544498(-) (pilB) [Pseudoalteromonas sp. Bsw20308]
ATGAATATTAACTCACCGTTACTAAGAAAATTTATTACCCTTGGCCGTATTGATGCAGATACCGTTAAAGTAAAACAACG
AGATTTTGCCTCAACCGCCGAGCTTATTGCCAAATGTGGAAACATCGATAGCAAAGATTTAGCTGAGCAATGTATTGATT
TATTTCGCGTACCTTATTTCGATTTAAAAGACTTTGAACCTGCAAGGATCCCAGCAGAACTTGTAAAAGAAAAGTTGATC
CGTAAACACCATATTTTACCCTTGGTGCAAAAAGGTAGAAAAGTATACATAGCCGCGTCTGATCCGACCGATTATGGTGC
GTTTGAAAACTATGAATTTAGTACCGGCTTATCCTGTGAAATAGTAGTAGTTGACTACATTGAACTTGATAAAAAAATTG
ACCAATTGCTTGATGCAACTGGTAGCTTAAATTTAAGTGATGACGAATTTAAAGAATTTGCTGATTTAGAGAGTGAAAAC
ACTAAAGAAGCCACCCCAAAAGATGATGAAAAAGATGATGCACCTATCATTGTTTACATCAATAAAATCCTAATGGATGC
CATTAAAAAAGGCGCCTCTGATTTACACTTTGAACCTTATGAGCATAAGTATCGCATCCGTTTTCGTATTGATGGTATTT
TGCATGAGGTAGCCAGCCCGCCTAATACTTTAGCGACAAAACTTTCTGCACGAATAAAAGTAATGTCACGCCTAGATATT
GCCGAAAAACGTAAACCACAAGATGGCCGTATAAAGCTAAAAATTACTGAGCGTAAAAGTATCGATTTTCGTGTAAGTAC
AATGCCGACCCTGTGGGGGGAAAAAATAGTAATGCGTATTCTTGATTCATCAAGCGCAATGCTAGGTATTGATGTATTAG
GCTACGAGCCGGAGCAGAAAAAACTCTATATGGATGCGCTTGCTCAGCCTCAAGGGATGATTTTAGTTACAGGACCAACG
GGCTCGGGTAAAACAGTATCACTTTACACTGGTTTAAATATACTTAATCAACCAGAGCGTAATATTAGTACCGCAGAGGA
TCCTGTCGAAATTAATCTCGAAGGTATAAACCAAGTACAAATTAATATTAAAGCCGATATGACCTTTGCAAATGCATTAC
GTGCATTTTTACGCCAAGATCCTGATGTCGTCATGGTAGGTGAAATACGTGACCTCGAAACAGCTGAAATATCAATAAAA
GCGGCGCAAACAGGTCACTTAGTACTATCAACTCTGCATACTAATTCAGCACCTGAAACGATAACTCGGTTATTAAATAT
GGGCGTACCAGCTTATAACGTAGCAAGTTCAATTAGTTTGATTATAGCCCAGCGCTTGGCTCGACGTTTATGCCCTAAAT
GTAAAACACCGGAAATATTACCAAACGAAGAACTTACCCGCCAAGGTTTTAGCGAGCAACAAATTAGTGAAATGACCCTC
TATGCGCCTAAAGGATGTGATAATTGCACTGATGGTTATAAGGGTCGTGTCGGTATTTATGAAGTAATGCAAATAACACC
AGAGATAGCACAAATTATAATGCGTGGCGGTAATTCATTAGAAATAGCAGAAGTATCACTTAAAGCAGGATTTAATAACT
TGCGATTATCAGGTATTCGTAAAGCGGCTGATGGAATGACCTCTCTTGCTGAAATTAATCGAGTAACAAGTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(182-449)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.454

91.935

0.547
  pilB Acinetobacter baylyi ADP1

58.218

90.502

0.527
  pilB Legionella pneumophila strain ERS1305867

53.172

96.057

0.511
  pilB Vibrio cholerae strain A1552

52.142

96.237

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

52.247

95.699

0.5
  pilB Vibrio campbellii strain DS40M4

51.963

95.878

0.498
  pilF Neisseria gonorrhoeae MS11

50

93.548

0.468

Multiple sequence alignment