Detailed information NTG0160262

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: D172_RS02310
• Genome accession: NZ_CP013138
• Coordinates: 545426..546310 (-)
• Length: 294 a.a.
• NCBI ID: WP_007376052.1
• Uniprot ID: A0AB73BI98
• Organism: Pseudoalteromonas sp. Bsw20308
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 540426..551310

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D172_RS02285 (D172_002285)	-	541036..541725 (-)	690	WP_007376047.1	response regulator	-
D172_RS02290 (D172_002290)	-	541915..542403 (+)	489	WP_007376048.1	periplasmic heavy metal sensor	-
D172_RS02295 (D172_002295)	yacG	542476..542706 (-)	231	WP_007376049.1	DNA gyrase inhibitor YacG	-
D172_RS02300 (D172_002300)	pilB	542822..544498 (-)	1677	WP_007376050.1	type IV-A pilus assembly ATPase PilB	Machinery gene
D172_RS02305 (D172_002305)	coaE	544777..545424 (-)	648	WP_007376051.1	dephospho-CoA kinase	-
D172_RS02310 (D172_002310)	pilD	545426..546310 (-)	885	WP_007376052.1	prepilin peptidase	Machinery gene
D172_RS02315 (D172_002315)	pilC	546326..547555 (-)	1230	WP_007376053.1	type II secretion system F family protein	Machinery gene
D172_RS02320 (D172_002320)	-	547649..548992 (-)	1344	WP_007376054.1	O-antigen ligase family protein	-
D172_RS02325 (D172_002325)	-	549055..549474 (-)	420	WP_007376055.1	pilin	-
D172_RS02330 (D172_002330)	nadC	549936..550781 (-)	846	WP_033023262.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 294 a.a.
• Molecular weight: 32731.02 Da
• Isoelectric Point: 5.9047

>NTDB_id=160262 D172_RS02310 WP_007376052.1 545426..546310(-) (pilD) [Pseudoalteromonas sp. Bsw20308]
MQSIVEVMQNELWFYLTTVGLVSLCVGSFLNVVIYRLPLMMQKEWQSECRILLEDELKNSKAINNEQFNLVKPNSTCPKC
KTAIKPWQNIPVISWLFLKGKCASCNNPISIRYPAVEAITAILSLFIAYTMGANEQALLYILITWALVALTFIDIDHMLL
PDQLTLPLVWLALIASIMGYTVAPSDAIMGAVFGYLSLWSVFWLFKLLTGKEGMGYGDFKLLAVFGALLGWQSLLSIILL
SSVVGAVIGIVLLSIQGKDKATPIPFGPYLAIAGWITMLWGTELQNIYFNLIGY

Nucleotide

• Length: 885 bp

>NTDB_id=160262 D172_RS02310 WP_007376052.1 545426..546310(-) (pilD) [Pseudoalteromonas sp. Bsw20308]
ATGCAAAGTATTGTAGAAGTAATGCAAAATGAGCTGTGGTTTTATCTAACCACCGTTGGCTTGGTTAGTTTATGTGTGGG
CAGCTTTTTAAATGTAGTGATTTATCGCCTACCACTTATGATGCAAAAAGAATGGCAGAGTGAGTGCAGAATACTGTTAG
AAGACGAGCTTAAAAATAGTAAAGCAATAAATAACGAGCAATTTAATTTAGTAAAGCCAAACTCAACCTGCCCAAAGTGC
AAAACGGCTATTAAACCTTGGCAAAATATTCCTGTTATTAGCTGGTTATTTTTAAAAGGAAAATGTGCAAGTTGTAATAA
CCCTATATCAATACGCTATCCTGCAGTCGAAGCCATTACAGCAATATTAAGCTTATTTATTGCTTATACTATGGGCGCAA
ATGAACAAGCTTTACTGTATATATTGATTACTTGGGCATTAGTTGCACTAACCTTTATTGATATAGACCATATGCTATTA
CCCGATCAACTTACATTACCACTAGTATGGCTTGCTTTAATAGCGTCAATAATGGGCTATACCGTAGCGCCGAGCGATGC
AATTATGGGGGCGGTTTTTGGTTATTTGAGCCTATGGAGTGTATTTTGGCTATTTAAGTTACTAACGGGTAAAGAAGGTA
TGGGCTATGGCGACTTTAAATTACTCGCTGTATTTGGTGCATTACTTGGTTGGCAATCACTACTTAGCATTATATTACTT
TCAAGTGTTGTTGGCGCTGTTATTGGCATTGTATTACTCAGTATTCAAGGAAAAGACAAAGCAACCCCGATTCCATTTGG
TCCTTACCTTGCCATTGCAGGCTGGATCACTATGCTATGGGGCACTGAATTACAAAACATTTATTTTAATTTAATTGGTT
ATTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (142-250)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (22-130)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	51.557	98.299	0.507
pilD	Acinetobacter baumannii D1279779	50.69	98.639	0.5
pilD	Acinetobacter nosocomialis M2	50.345	98.639	0.497
pilD	Vibrio cholerae strain A1552	50.896	94.898	0.483
pilD	Neisseria gonorrhoeae MS11	46.388	89.456	0.415