Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AOT11_RS09835 Genome accession   NZ_CP012881
Coordinates   2086559..2088244 (-) Length   561 a.a.
NCBI ID   WP_017421193.1    Uniprot ID   -
Organism   Vibrio vulnificus NBRC 15645 = ATCC 27562     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2081559..2093244
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOT11_RS09805 (AOT11_16840) rplS 2082017..2082370 (+) 354 WP_011079544.1 50S ribosomal protein L19 -
  AOT11_RS09810 (AOT11_16845) yacG 2082682..2082876 (-) 195 WP_026060801.1 DNA gyrase inhibitor YacG -
  AOT11_RS09815 (AOT11_16850) zapD 2082939..2083679 (-) 741 WP_017421189.1 cell division protein ZapD -
  AOT11_RS09820 (AOT11_16855) coaE 2083712..2084320 (-) 609 WP_017421190.1 dephospho-CoA kinase -
  AOT11_RS09825 (AOT11_16860) pilD 2084322..2085191 (-) 870 WP_026060802.1 prepilin peptidase Machinery gene
  AOT11_RS09830 (AOT11_16865) pilC 2085285..2086511 (-) 1227 WP_017421192.1 type II secretion system F family protein Machinery gene
  AOT11_RS09835 (AOT11_16870) pilB 2086559..2088244 (-) 1686 WP_017421193.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AOT11_RS09840 (AOT11_16875) - 2088249..2088698 (-) 450 WP_176309687.1 pilin -
  AOT11_RS09845 (AOT11_16880) nadC 2088921..2089808 (-) 888 WP_017421195.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AOT11_RS09850 (AOT11_16885) ampD 2089901..2090473 (+) 573 WP_017421196.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AOT11_RS09855 (AOT11_16890) pdhR 2090862..2091629 (+) 768 WP_011079554.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62342.33 Da        Isoelectric Point: 5.6848

>NTDB_id=158043 AOT11_RS09835 WP_017421193.1 2086559..2088244(-) (pilB) [Vibrio vulnificus NBRC 15645 = ATCC 27562]
MTNLATILRQANQLSLTQEQACRENIQASGVTAPEALLQLGFFQPDELTEKLSAIFGLPRTDLNQYDYQELCNQLGLREL
ITRHQALPLQRSNAGLILAVADPTNIQAEDDFRFATGVQVEIVLADCQDLQAAIRRLYGKSIKQSQGSGLKEIHQDELAN
LVEVSDDEVESIEDLSQDESPVSRFINQILLDAVRKGASDIHFEPYENQYRVRLRCDGILVEVQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLRLNQETAIDMRVSTLPTLFGEKIVLRLLDSSSSSLDIDKLGYNPQQKQLYLDALRKPQGMI
LMTGPTGSGKTVSLYTGLSLLNRPEVNISTAEDPVEINLPGINQVQVKPKIGFGFAEALRSFLRQDPDIVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCSHCKQPHPYNATFELLGLKADDL
IYQADRQGCHHCTHGYSGRVGIYEVMPFDEQLAEAIVKGASVQSLEMLARQKGMMTLKDSGLEKLKQGITSLEELQRVLY
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=158043 AOT11_RS09835 WP_017421193.1 2086559..2088244(-) (pilB) [Vibrio vulnificus NBRC 15645 = ATCC 27562]
ATGACTAACCTTGCCACAATTCTTCGTCAGGCCAATCAACTCAGCCTAACGCAAGAACAAGCGTGTCGAGAAAACATCCA
AGCTTCTGGTGTGACAGCACCGGAAGCTTTGTTGCAACTGGGATTTTTCCAGCCTGATGAACTAACCGAAAAGCTAAGTG
CGATTTTTGGCTTACCACGCACTGACCTCAACCAATATGACTACCAAGAGCTGTGTAATCAACTTGGGTTGCGTGAGCTG
ATCACTCGTCACCAAGCACTGCCACTGCAAAGAAGCAACGCTGGTTTAATACTCGCGGTTGCAGACCCGACCAATATTCA
AGCTGAAGATGATTTCCGTTTTGCCACAGGCGTGCAGGTGGAGATTGTTTTAGCCGATTGTCAAGATCTGCAAGCGGCGA
TTCGTAGACTCTATGGAAAGAGCATCAAACAGAGCCAAGGCTCTGGTTTAAAAGAGATTCACCAAGATGAACTGGCGAAT
TTGGTCGAGGTTTCAGATGACGAAGTCGAGTCCATTGAAGACTTAAGCCAAGATGAATCCCCCGTTAGCCGCTTTATCAA
TCAAATTCTGCTTGATGCGGTGCGCAAAGGCGCGTCCGATATTCATTTTGAACCGTACGAAAACCAATACCGCGTACGAT
TGCGTTGCGATGGTATTTTGGTGGAAGTGCAACAGCCTCCAAGCCATTTAAGCCGCCGTTTATCCGCGAGGATCAAAATC
CTCTCCAAGCTGGATATTGCGGAAAGGCGTCTGCCACAAGATGGACGCATTAAGCTACGCCTTAACCAAGAAACCGCGAT
TGATATGCGCGTTTCCACTTTACCTACGCTGTTTGGTGAAAAAATTGTGCTGCGACTTCTCGACAGCTCGAGTAGCAGCT
TGGATATTGATAAGTTAGGCTACAACCCGCAACAAAAGCAGCTCTACCTCGATGCCCTGCGCAAACCGCAAGGAATGATT
TTGATGACAGGTCCAACGGGCAGCGGCAAAACCGTTTCGCTGTACACAGGCTTGAGTTTGCTTAACCGACCTGAAGTGAA
TATCTCCACCGCGGAAGACCCGGTCGAAATTAACCTCCCGGGAATCAACCAAGTGCAAGTGAAACCCAAAATTGGTTTTG
GTTTTGCCGAAGCATTGCGTTCTTTTCTCCGTCAAGACCCGGATATCGTCATGGTGGGGGAAATTCGAGATTTGGAAACC
GCCGAGATCGCCGTCAAAGCAGCCCAAACCGGCCACCTTGTGCTATCCACCTTACATACCAACTCGGCTGCTGAAACCAT
TGTTCGTCTATCTAACATGGGGATCGCCAGCTTTAACTTGGCTTCATCCTTGAGTTTGATCATTGCTCAGCGTCTGGCTC
GTCGGTTATGCAGCCATTGCAAGCAACCTCATCCATACAACGCAACATTTGAGTTACTCGGTCTAAAAGCTGACGACTTG
ATTTATCAAGCTGATAGACAGGGTTGCCATCATTGTACTCATGGTTATTCCGGTCGTGTCGGTATATACGAAGTCATGCC
TTTTGATGAACAGCTTGCAGAGGCGATAGTGAAAGGCGCCTCGGTACAAAGCTTGGAGATGCTCGCTCGGCAAAAAGGCA
TGATGACACTCAAAGATTCCGGCCTTGAAAAACTCAAACAGGGCATCACCAGTTTAGAAGAGCTGCAACGCGTCCTCTAT
TTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

76.649

100

0.766
  pilB Vibrio parahaemolyticus RIMD 2210633

75.893

99.822

0.758
  pilB Vibrio cholerae strain A1552

74.199

100

0.743
  pilB Legionella pneumophila strain ERS1305867

53.216

91.444

0.487
  pilB Acinetobacter baumannii D1279779

51.538

92.692

0.478
  pilB Acinetobacter baylyi ADP1

50.478

93.226

0.471
  pilF Neisseria gonorrhoeae MS11

45.633

100

0.456
  pilF Thermus thermophilus HB27

40.215

99.287

0.399
  pilB/pilB1 Synechocystis sp. PCC 6803

37.063

100

0.378
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.679

94.474

0.365
  pilB Haemophilus influenzae Rd KW20

41.736

86.275

0.36

Multiple sequence alignment