Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AOT11_RS08645 Genome accession   NZ_CP012881
Coordinates   1852003..1853787 (+) Length   594 a.a.
NCBI ID   WP_017419660.1    Uniprot ID   A0A087IG05
Organism   Vibrio vulnificus NBRC 15645 = ATCC 27562     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1847003..1858787
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOT11_RS08625 (AOT11_15660) pilM 1849317..1850324 (+) 1008 WP_017419656.1 type IV pilus assembly protein PilM Machinery gene
  AOT11_RS08630 (AOT11_15665) pilN 1850314..1850904 (+) 591 WP_017419657.1 PilN domain-containing protein Machinery gene
  AOT11_RS08635 (AOT11_15670) pilO 1850897..1851487 (+) 591 WP_011079354.1 type 4a pilus biogenesis protein PilO Machinery gene
  AOT11_RS08640 (AOT11_15675) pilP 1851477..1851989 (+) 513 WP_017419659.1 pilus assembly protein PilP Machinery gene
  AOT11_RS08645 (AOT11_15680) pilQ 1852003..1853787 (+) 1785 WP_017419660.1 type IV pilus secretin PilQ Machinery gene
  AOT11_RS08650 (AOT11_15685) aroK 1854027..1854545 (+) 519 WP_011079357.1 shikimate kinase AroK -
  AOT11_RS08655 (AOT11_15690) aroB 1854578..1855669 (+) 1092 WP_011079358.1 3-dehydroquinate synthase -
  AOT11_RS08660 (AOT11_15695) - 1855720..1857216 (+) 1497 WP_017419661.1 AAA family ATPase -
  AOT11_RS08665 (AOT11_15700) - 1857296..1858123 (+) 828 WP_017419662.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 594 a.a.        Molecular weight: 64591.66 Da        Isoelectric Point: 4.5077

>NTDB_id=158031 AOT11_RS08645 WP_017419660.1 1852003..1853787(+) (pilQ) [Vibrio vulnificus NBRC 15645 = ATCC 27562]
MICELMMKNKIQLFPGMLSQFVCLLSVCIFTTFANAQSEAENALKSIDFRTNAAKDAIIIVELATPATVVDLKPVAEGLS
IELINTSVKDEQLYLLDVKDFSTVVESIEVFREVSKTRLVANVSGEFRHDYRLSGRYLEVTISRIKSEQEAREQSVLEKE
GKLISINFQDIPVRNVLQLIADYNGFNLVVSDSVTGNVTLRLDGVPWQQVLDIILQVKGLDKRVEGNVVLIAPQDELDLR
EKQQLEKERLAEELGSLKSEIIKVNFAKASDIAEMINGKESVSMLSERGSVAIDERTNSLLLRELPENIEVIREIINSLD
IPVKQVQIEARIVTVNEGNLDELGVRWGFTSINGSTTVGGSIENNLATIGLYNAGSGSGSGDSGSGGSGDEGGSNSIDDF
LNVNLAATSANASSIAFQVAKLGSDLLLDLELSALQRESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVSF
KKAVLSLKVTPQITPDNRLVLDLSVTQDRPGEIVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNTTDKVPL
LGDLPLLGALFRRSYENVGKRELLIFVTPKVILQ

Nucleotide


Download         Length: 1785 bp        

>NTDB_id=158031 AOT11_RS08645 WP_017419660.1 1852003..1853787(+) (pilQ) [Vibrio vulnificus NBRC 15645 = ATCC 27562]
GTGATTTGTGAGTTGATGATGAAAAACAAAATCCAATTGTTTCCGGGAATGCTTTCCCAATTCGTCTGCCTGTTGTCTGT
TTGCATCTTTACCACTTTTGCAAACGCACAATCTGAAGCAGAAAATGCGCTCAAGAGCATCGATTTCAGAACCAATGCAG
CCAAAGATGCGATTATCATCGTTGAATTGGCAACGCCTGCAACCGTGGTGGATTTGAAACCAGTGGCAGAAGGATTGAGC
ATCGAGTTGATCAATACCTCGGTTAAAGATGAGCAGCTCTATCTGCTCGATGTCAAAGATTTTTCGACCGTGGTTGAAAG
TATCGAAGTATTCAGAGAAGTGTCCAAAACCCGTCTTGTTGCGAATGTCAGCGGTGAATTTCGTCACGACTATCGTCTTT
CTGGACGCTATCTCGAGGTGACAATTTCTCGCATCAAATCGGAGCAAGAAGCCCGTGAACAAAGTGTGTTGGAAAAAGAA
GGCAAGCTGATTTCTATCAACTTCCAAGATATTCCAGTGCGTAACGTTTTGCAGCTTATCGCAGACTACAATGGCTTCAA
CCTCGTGGTGTCTGATTCTGTCACTGGTAATGTGACGCTGAGATTGGATGGGGTACCTTGGCAGCAAGTGCTCGACATCA
TTCTTCAAGTGAAAGGTCTAGACAAACGTGTTGAAGGCAATGTTGTGCTCATTGCTCCTCAAGATGAACTGGATTTACGA
GAGAAGCAGCAGCTTGAGAAAGAACGTCTGGCGGAAGAGCTTGGCTCCCTCAAATCGGAAATCATTAAAGTCAACTTTGC
CAAAGCGTCAGACATTGCTGAGATGATCAATGGCAAAGAGTCGGTCAGCATGCTGTCAGAACGTGGTAGTGTGGCGATTG
ATGAGCGGACAAACTCTCTTTTGTTGCGTGAGTTACCCGAAAACATCGAAGTGATTCGAGAAATTATCAATTCGTTGGAT
ATCCCAGTCAAACAGGTACAAATTGAAGCACGCATTGTCACGGTGAATGAAGGCAATCTTGATGAGCTTGGTGTCCGTTG
GGGGTTCACCTCCATCAACGGCAGCACAACCGTCGGCGGTTCTATCGAAAACAACCTAGCAACCATTGGTCTCTACAATG
CAGGCAGTGGGAGTGGCAGTGGTGACTCAGGTAGCGGTGGCAGTGGTGATGAAGGCGGGTCGAACTCCATTGATGATTTC
CTCAATGTCAATCTTGCTGCGACTTCTGCAAACGCCTCAAGTATTGCTTTCCAAGTGGCTAAATTGGGGTCGGATTTGTT
GTTGGATCTCGAGCTATCAGCACTGCAACGCGAATCAAAAGCGGAAATTATTTCTAGCCCTCGTTTGATCACAACCAATA
AGAAGCCTGCTTATATCGAGCAAGGTACAGAGATTCCTTATCTAGAGTCTTCCTCCAGTGGAGCGACCACGGTTTCCTTT
AAGAAAGCTGTGCTTAGCCTGAAAGTAACCCCTCAGATCACGCCTGACAATCGTCTCGTGTTGGATTTAAGCGTTACACA
AGATAGACCTGGCGAAATTGTCAAAACAGGTACTGGGGAAGCGGTTGCGATTGATACTCAGCGCATCGGTACACAAGTCT
TGGTCAATAATGGTGAAACGGTAGTGTTAGGCGGTATTTTCCAACACAGCATAACCAATACCACGGACAAGGTTCCTCTG
TTAGGTGATTTACCCTTATTGGGTGCGCTTTTCCGCCGTAGCTATGAAAACGTGGGCAAGCGTGAATTGCTGATATTTGT
CACACCTAAGGTTATCTTGCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(260-325)

Bacterial type II and III secretion system protein (Secretin)

(434-592)

Secretin and TonB N terminus short domain (STN)

(186-234)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A087IG05

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

77.966

99.327

0.774
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

74.068

99.327

0.736
  pilQ Vibrio cholerae strain A1552

74.068

99.327

0.736
  pilQ Legionella pneumophila strain Lp02

41.429

94.276

0.391
  pilQ Legionella pneumophila strain ERS1305867

41.429

94.276

0.391
  pilQ Pseudomonas aeruginosa PAK

38.435

96.801

0.372
  comQ Acinetobacter baylyi ADP1

36.824

99.663

0.367

Multiple sequence alignment