Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AOT11_RS08625 Genome accession   NZ_CP012881
Coordinates   1849317..1850324 (+) Length   335 a.a.
NCBI ID   WP_017419656.1    Uniprot ID   A0A087I1V6
Organism   Vibrio vulnificus NBRC 15645 = ATCC 27562     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1844317..1855324
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOT11_RS08610 (AOT11_15645) - 1845150..1845422 (-) 273 WP_017419653.1 DUF3624 domain-containing protein -
  AOT11_RS08615 (AOT11_15650) oxyR 1845696..1846595 (+) 900 WP_026050199.1 DNA-binding transcriptional regulator OxyR -
  AOT11_RS08620 (AOT11_15655) - 1846664..1849177 (-) 2514 WP_017419655.1 penicillin-binding protein 1A -
  AOT11_RS08625 (AOT11_15660) pilM 1849317..1850324 (+) 1008 WP_017419656.1 type IV pilus assembly protein PilM Machinery gene
  AOT11_RS08630 (AOT11_15665) pilN 1850314..1850904 (+) 591 WP_017419657.1 PilN domain-containing protein Machinery gene
  AOT11_RS08635 (AOT11_15670) pilO 1850897..1851487 (+) 591 WP_011079354.1 type 4a pilus biogenesis protein PilO Machinery gene
  AOT11_RS08640 (AOT11_15675) pilP 1851477..1851989 (+) 513 WP_017419659.1 pilus assembly protein PilP Machinery gene
  AOT11_RS08645 (AOT11_15680) pilQ 1852003..1853787 (+) 1785 WP_017419660.1 type IV pilus secretin PilQ Machinery gene
  AOT11_RS08650 (AOT11_15685) aroK 1854027..1854545 (+) 519 WP_011079357.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 335 a.a.        Molecular weight: 37654.05 Da        Isoelectric Point: 6.3873

>NTDB_id=158027 AOT11_RS08625 WP_017419656.1 1849317..1850324(+) (pilM) [Vibrio vulnificus NBRC 15645 = ATCC 27562]
MGKPFVTGIDIGHRSLKAVILKPYGEHYALLGYKEIIIKDGIFSDNHTLNHQEIVNSLKELKKELPLFRYQVSLAVPDSS
VISKMLSIDAELDGREREFAILQAFAHQSPFPVDELSIDYVQLVEETSKTNHSYQVYATKKEVVESRVRVAEEVGLKPVL
VDVHAHSLCNILTLAAERHPDKASYCLLDVGYDTTSLVMSRRNEPPLVKEFAYGVSHLNASIGDDLTSPDAHEKTDIFNK
ELTERVRRQLQLFQSMDSQSKVNGLWICGEGAITPLLSETLQRKLSLECEVLNPLALFEQKTAKRKRRIVDWHHFSVAAG
LAIGGIQWLEDSHAA

Nucleotide


Download         Length: 1008 bp        

>NTDB_id=158027 AOT11_RS08625 WP_017419656.1 1849317..1850324(+) (pilM) [Vibrio vulnificus NBRC 15645 = ATCC 27562]
ATGGGTAAACCGTTCGTTACCGGAATTGATATTGGTCATCGTAGCCTCAAGGCTGTCATTCTGAAACCTTATGGAGAGCA
CTACGCCCTGTTAGGGTACAAAGAAATAATCATTAAAGACGGTATTTTCTCAGATAACCACACTCTGAATCATCAAGAAA
TTGTCAATAGCCTGAAAGAACTGAAGAAGGAGTTGCCTCTTTTCCGTTATCAGGTGTCACTGGCGGTACCCGATAGTTCA
GTGATTAGTAAGATGTTATCGATCGATGCGGAGTTGGATGGTCGAGAACGAGAGTTCGCTATCCTTCAGGCGTTTGCACA
TCAGTCGCCATTTCCTGTCGATGAATTATCGATTGATTATGTCCAATTGGTCGAAGAAACCAGCAAAACGAATCATAGCT
ATCAAGTTTACGCCACCAAAAAAGAGGTGGTGGAAAGCCGAGTGCGTGTCGCAGAAGAAGTGGGACTGAAACCCGTGCTG
GTAGATGTACATGCGCATAGCCTGTGCAACATCTTAACGTTAGCGGCAGAGCGCCATCCAGACAAAGCCAGCTATTGCTT
GTTGGATGTGGGTTATGACACCACCTCTTTGGTGATGTCGCGTCGCAACGAACCACCTTTGGTTAAAGAGTTTGCCTATG
GGGTGTCTCACCTCAATGCGTCGATAGGCGACGATCTGACTTCTCCTGATGCTCATGAGAAAACCGACATCTTTAATAAA
GAGCTGACAGAGCGAGTTCGTCGGCAATTGCAGTTGTTCCAATCCATGGATAGCCAAAGCAAAGTGAATGGCCTTTGGAT
CTGCGGTGAAGGCGCGATTACGCCGTTGTTGAGTGAAACTTTGCAGAGAAAGCTGAGTTTGGAGTGTGAAGTCCTTAATC
CTCTGGCTCTATTTGAGCAAAAAACGGCGAAAAGAAAGCGCCGTATTGTTGATTGGCACCATTTTTCGGTCGCCGCGGGA
TTAGCGATTGGTGGCATTCAGTGGCTGGAGGATAGCCATGCAGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A087I1V6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

53.134

100

0.531
  pilM Vibrio cholerae strain A1552

48.974

100

0.499

Multiple sequence alignment