Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FORC17_RS02790 Genome accession   NZ_CP012739
Coordinates   567169..568857 (-) Length   562 a.a.
NCBI ID   WP_065090097.1    Uniprot ID   -
Organism   Vibrio vulnificus strain FORC_017     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 562169..573857
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC17_RS02760 (FORC17_0481) rplS 562675..563028 (+) 354 WP_011079544.1 50S ribosomal protein L19 -
  FORC17_RS02765 (FORC17_0482) yacG 563291..563485 (-) 195 WP_065090094.1 DNA gyrase inhibitor YacG -
  FORC17_RS02770 (FORC17_0483) zapD 563548..564288 (-) 741 WP_011079546.1 cell division protein ZapD -
  FORC17_RS02775 (FORC17_0484) coaE 564321..564929 (-) 609 WP_065090095.1 dephospho-CoA kinase -
  FORC17_RS02780 (FORC17_0485) pilD 564931..565800 (-) 870 WP_065090096.1 prepilin peptidase Machinery gene
  FORC17_RS02785 (FORC17_0486) pilC 565894..567120 (-) 1227 WP_011079549.1 type II secretion system F family protein Machinery gene
  FORC17_RS02790 (FORC17_0487) pilB 567169..568857 (-) 1689 WP_065090097.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FORC17_RS02795 (FORC17_0488) pilA 568866..569288 (-) 423 WP_065090098.1 type IV pilin protein Machinery gene
  FORC17_RS02800 (FORC17_0489) nadC 569511..570398 (-) 888 WP_065090099.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FORC17_RS02805 (FORC17_0490) ampD 570491..571063 (+) 573 WP_011079553.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FORC17_RS02810 (FORC17_0491) pdhR 571452..572219 (+) 768 WP_011079554.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62344.35 Da        Isoelectric Point: 5.5330

>NTDB_id=157436 FORC17_RS02790 WP_065090097.1 567169..568857(-) (pilB) [Vibrio vulnificus strain FORC_017]
MPSNLATILRQANQLGLTQEQACRETMQASGVTAPEALLQLGFFQPDELTEQLSAIFGLPRTDLNQYDYQDLCNQLGLRE
LITRHQALPLQKSNAGLILAVADPTNIQAEDDFRFATGVQVEIVLADCQELQAAIRRLYGKSIKQSQGSGLKEIHQDELA
NLVEVSDDEVESIEDLSQDESPVSRFINQILLDAVRKGASDIHFEPYENQYRVRLRCDGILVEVQQPPSHLSRRLSARIK
ILSKLDIAERRLPQDGRIKLRLNQETAIDMRVSTLPTLFGEKIVLRLLDSSSSSLDIDKLGYNPQQKQLYLDALRKPQGM
ILMTGPTGSGKTVSLYTGLSLLNRPEVNISTAEDPVEINLPGINQVQVKPKIGFGFAEALRSFLRQDPDIVMVGEIRDLE
TAEIAVKAAQTGHLVLSTLHTNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCSHCKQPHPYNATFELLGLKADD
LIYQADKQGCHHCTHGYSGRVGIYEVMPFDEQLAEAIVKGASVQSLEMLARQKGMMTLKDSGLEKLKQGITSLEELQRVL
YL

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=157436 FORC17_RS02790 WP_065090097.1 567169..568857(-) (pilB) [Vibrio vulnificus strain FORC_017]
ATGCCCTCCAATCTTGCCACTATTCTTCGTCAGGCCAATCAACTTGGCCTAACGCAAGAACAAGCGTGTCGAGAGACAAT
GCAAGCTTCTGGTGTGACAGCACCGGAAGCTTTGTTGCAACTGGGATTTTTCCAGCCGGATGAATTAACGGAGCAGCTCA
GCGCGATTTTTGGCTTACCACGCACTGACCTCAACCAATATGACTACCAAGATCTGTGTAATCAACTTGGGTTACGTGAG
CTGATCACTCGTCACCAAGCACTGCCACTGCAAAAAAGCAACGCTGGTTTAATACTCGCGGTTGCTGACCCTACCAATAT
TCAAGCTGAAGATGATTTCCGTTTTGCCACAGGTGTGCAGGTGGAGATTGTTTTGGCCGATTGCCAAGAGCTGCAAGCGG
CGATTCGTAGACTCTATGGAAAGAGCATCAAACAGAGCCAAGGCTCTGGTTTAAAAGAGATTCACCAAGATGAGCTGGCG
AATTTAGTCGAGGTTTCAGATGACGAAGTCGAGTCAATTGAAGACTTAAGCCAAGATGAATCTCCCGTTAGCCGCTTTAT
CAACCAAATTCTGCTGGATGCGGTGCGCAAAGGCGCATCTGATATTCATTTTGAACCGTACGAAAACCAATACCGCGTGC
GATTGCGTTGCGATGGTATTTTGGTGGAAGTGCAGCAGCCTCCAAGCCATTTAAGCCGCCGTTTATCCGCGAGGATCAAG
ATCCTCTCCAAGCTGGATATTGCGGAAAGGCGTCTGCCACAAGATGGACGTATTAAGCTGCGCCTCAACCAAGAAACCGC
GATTGATATGCGCGTTTCCACCTTACCCACACTGTTTGGTGAAAAAATTGTGTTGCGACTTCTCGACAGCTCGAGCAGTA
GCTTGGATATTGATAAGTTAGGCTACAACCCGCAACAAAAACAGCTCTACCTCGATGCCCTGCGCAAACCGCAAGGGATG
ATTTTGATGACGGGCCCAACAGGAAGCGGCAAAACCGTTTCGCTGTACACAGGCTTGAGTTTACTTAACCGGCCAGAAGT
GAATATCTCCACCGCGGAAGACCCAGTCGAGATTAACCTCCCGGGAATCAACCAAGTGCAAGTGAAACCCAAAATTGGTT
TTGGTTTTGCAGAAGCATTGCGTTCTTTTCTCCGTCAAGACCCGGATATCGTCATGGTGGGGGAAATTCGAGATTTGGAA
ACCGCCGAGATCGCCGTCAAAGCAGCCCAAACCGGTCACCTTGTGCTATCCACCTTACATACCAACTCGGCAGCTGAAAC
CATTGTTCGTCTATCTAACATGGGGATCGCCAGCTTTAACTTGGCCTCATCCTTGAGTTTGATCATTGCTCAGCGTCTGG
CTCGTCGGTTATGCAGCCATTGCAAGCAACCTCATCCATACAACGCAACATTTGAGTTACTCGGTCTAAAAGCTGACGAC
TTGATTTATCAAGCTGATAAACAGGGTTGCCATCATTGTACTCATGGTTATTCCGGTCGTGTCGGTATATACGAAGTCAT
GCCTTTTGATGAACAGCTTGCAGAGGCGATCGTTAAAGGCGCCTCGGTACAAAGCTTGGAGATGCTTGCTCGGCAAAAAG
GCATGATGACACTCAAAGATTCCGGCCTTGAAAAACTCAAACAGGGCATCACCAGCTTAGAAGAGCTGCAACGTGTCCTC
TATTTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

76.512

100

0.765
  pilB Vibrio parahaemolyticus RIMD 2210633

75.979

100

0.76
  pilB Vibrio cholerae strain A1552

74.245

100

0.744
  pilB Legionella pneumophila strain ERS1305867

53.216

91.281

0.486
  pilB Acinetobacter baumannii D1279779

51.341

92.883

0.477
  pilB Acinetobacter baylyi ADP1

50.383

92.883

0.468
  pilF Neisseria gonorrhoeae MS11

45.31

100

0.456
  pilF Thermus thermophilus HB27

41.219

99.288

0.409
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.491

94.306

0.363

Multiple sequence alignment