Detailed information NTG0157434

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: FORC17_RS02780
• Genome accession: NZ_CP012739
• Coordinates: 564931..565800 (-)
• Length: 289 a.a.
• NCBI ID: WP_065090096.1
• Uniprot ID: B7U4S5
• Organism: Vibrio vulnificus strain FORC_017
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 559931..570800

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FORC17_RS02745 (FORC17_0478)	rpsP	561044..561292 (+)	249	WP_011079541.1	30S ribosomal protein S16	-
FORC17_RS02750 (FORC17_0479)	rimM	561308..561856 (+)	549	WP_061058912.1	ribosome maturation factor RimM	-
FORC17_RS02755 (FORC17_0480)	trmD	561884..562633 (+)	750	WP_011079543.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
FORC17_RS02760 (FORC17_0481)	rplS	562675..563028 (+)	354	WP_011079544.1	50S ribosomal protein L19	-
FORC17_RS02765 (FORC17_0482)	yacG	563291..563485 (-)	195	WP_065090094.1	DNA gyrase inhibitor YacG	-
FORC17_RS02770 (FORC17_0483)	zapD	563548..564288 (-)	741	WP_011079546.1	cell division protein ZapD	-
FORC17_RS02775 (FORC17_0484)	coaE	564321..564929 (-)	609	WP_065090095.1	dephospho-CoA kinase	-
FORC17_RS02780 (FORC17_0485)	pilD	564931..565800 (-)	870	WP_065090096.1	prepilin peptidase	Machinery gene
FORC17_RS02785 (FORC17_0486)	pilC	565894..567120 (-)	1227	WP_011079549.1	type II secretion system F family protein	Machinery gene
FORC17_RS02790 (FORC17_0487)	pilB	567169..568857 (-)	1689	WP_065090097.1	type IV-A pilus assembly ATPase PilB	Machinery gene
FORC17_RS02795 (FORC17_0488)	pilA	568866..569288 (-)	423	WP_065090098.1	type IV pilin protein	Machinery gene
FORC17_RS02800 (FORC17_0489)	nadC	569511..570398 (-)	888	WP_065090099.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32261.29 Da
• Isoelectric Point: 6.4630

>NTDB_id=157434 FORC17_RS02780 WP_065090096.1 564931..565800(-) (pilD) [Vibrio vulnificus strain FORC_017]
MDALQYYPWLYIGLASLFGLLVGSFLNVVIYRLPKIMELEWRQECAESFPEYNITPPTETLTLSTPRSSCPSCHTPIRVR
DNIPVFSWLALRGKCHHCQTKISARYPFVEALSAFLCGLVAWKFGYAPITVALIGFTLVLIAATFIDLDTMLLPDQLTLP
LTWTGIALALLEISPVSLQDSVIGAMAGYLCLWSVYHLFRLLTGKEGMGYGDFKLLAALGAWLGWQYLPMIILLSSVVGL
IFGLIQLRLQKQGIEMAFPFGPYLAIAGWVALMWGDSLMSWYLNYLIGA

Nucleotide

• Length: 870 bp

>NTDB_id=157434 FORC17_RS02780 WP_065090096.1 564931..565800(-) (pilD) [Vibrio vulnificus strain FORC_017]
ATGGACGCGTTACAATACTACCCTTGGCTTTATATTGGTTTAGCTTCCCTGTTTGGCTTACTGGTAGGCAGCTTTCTTAA
CGTCGTCATCTACCGTCTACCGAAAATAATGGAACTGGAATGGCGCCAAGAGTGTGCAGAGTCCTTTCCTGAATACAACA
TCACACCACCGACAGAAACCCTAACATTAAGCACGCCGCGTTCAAGTTGCCCTTCGTGCCATACCCCAATACGAGTTCGA
GATAATATTCCTGTCTTTAGTTGGCTAGCTCTAAGAGGAAAATGTCACCATTGCCAGACGAAAATCAGTGCTCGCTATCC
TTTTGTTGAAGCACTGAGCGCCTTCTTGTGCGGCCTAGTCGCTTGGAAGTTCGGTTATGCACCGATTACCGTAGCACTGA
TTGGTTTTACTTTGGTGTTGATCGCCGCCACCTTCATCGACTTAGATACCATGCTACTGCCTGATCAACTGACACTGCCG
CTGACGTGGACAGGCATCGCGCTAGCGTTACTAGAGATAAGCCCAGTATCTCTGCAAGATTCAGTGATCGGTGCGATGGC
AGGCTATCTCTGCCTTTGGAGCGTTTATCACCTTTTCAGATTGCTGACAGGGAAAGAAGGCATGGGTTACGGAGACTTTA
AATTGCTTGCGGCACTTGGAGCTTGGCTCGGCTGGCAATACCTACCGATGATCATCTTACTTTCTTCGGTTGTCGGGCTG
ATCTTTGGTCTCATTCAACTCAGATTGCAAAAGCAAGGTATTGAGATGGCTTTTCCATTTGGGCCTTATCTGGCCATTGC
TGGTTGGGTCGCCTTGATGTGGGGAGATAGCTTGATGTCTTGGTATCTCAACTATCTTATAGGGGCGTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

• Type IV leader peptidase family (Peptidase_A24) (137-244)

3D structure

• AlphaFold DB: B7U4S5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	77.778	99.654	0.775
pilD	Vibrio cholerae strain A1552	71.875	99.654	0.716
pilD	Acinetobacter nosocomialis M2	54.348	95.502	0.519
pilD	Acinetobacter baumannii D1279779	54.348	95.502	0.519
pilD	Neisseria gonorrhoeae MS11	48	95.156	0.457