Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   AOR04_RS15320 Genome accession   NZ_CP012737
Coordinates   3408705..3409931 (+) Length   408 a.a.
NCBI ID   WP_096793818.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. 1_2015MBL_MicDiv isolate 15DKN1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3403705..3414931
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOR04_RS15300 (AOR04_15120) ampE 3404686..3405537 (-) 852 WP_096793816.1 beta-lactamase regulator AmpE -
  AOR04_RS15305 (AOR04_15125) ampD 3405551..3406105 (-) 555 WP_096793817.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AOR04_RS15310 (AOR04_15130) nadC 3406880..3407725 (+) 846 WP_096794146.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AOR04_RS21900 (AOR04_15135) - 3408186..3408644 (+) 459 WP_269467059.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AOR04_RS15320 (AOR04_15140) pilC 3408705..3409931 (+) 1227 WP_096793818.1 type II secretion system F family protein Machinery gene
  AOR04_RS15325 (AOR04_15145) pilD 3409947..3410831 (+) 885 WP_096793819.1 A24 family peptidase Machinery gene
  AOR04_RS15330 (AOR04_15150) coaE 3410833..3411480 (+) 648 WP_096793820.1 dephospho-CoA kinase -
  AOR04_RS15335 (AOR04_15155) pilB 3411759..3413435 (+) 1677 WP_096793821.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AOR04_RS15340 (AOR04_15160) yacG 3413552..3413782 (+) 231 WP_007376049.1 DNA gyrase inhibitor YacG -
  AOR04_RS15345 (AOR04_15165) - 3414065..3414553 (-) 489 WP_096793822.1 Spy/CpxP family protein refolding chaperone -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 44415.43 Da        Isoelectric Point: 9.7879

>NTDB_id=157410 AOR04_RS15320 WP_096793818.1 3408705..3409931(+) (pilC) [Pseudoalteromonas sp. 1_2015MBL_MicDiv isolate 15DKN1]
MSKKNKNDDLNTFQWIGVSTRGKRLEGEIAGISIALVKAQLRKQGITPSKVKRKPKPLFGVHTQKITPKDIALVTRQIAT
MLMAGVPLIQSIEMIASGSTNKSVTKLISAVGDDVKAGQPLSLSLRKYPRYFDDLYCDLVESGEQSGALDKIFDRIAIYK
EKSEALKSKIKKAMFYPIAVLVIALIVTSILLIFVVPQFQDIFAGFGAELPAFTLFVIGISDFMQESWWMALIAIVVFGW
TFKEAKLRSLKLRDATDRAILKLPVIGMILNKAAVARYARTLSTTFAAGVPLVDALDSAAGASGNAVYRYAILDIKAEVS
SGNQMNWAMRNSKIFPDMVIQMVAIGEESGSLDGMLAKVATIYEQEVDDAVDGLSSLLEPLIMAVLGVLVGGLIVAMYLP
IFQLGSVI

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=157410 AOR04_RS15320 WP_096793818.1 3408705..3409931(+) (pilC) [Pseudoalteromonas sp. 1_2015MBL_MicDiv isolate 15DKN1]
GTGTCTAAAAAAAATAAAAACGATGATTTAAATACTTTCCAATGGATAGGAGTTAGTACTAGAGGAAAGCGCTTAGAAGG
TGAAATCGCTGGTATCAGTATTGCCTTAGTAAAAGCTCAACTTCGTAAGCAAGGTATTACTCCCTCAAAAGTAAAACGTA
AACCAAAACCTTTATTTGGCGTACACACTCAAAAAATAACACCTAAAGATATCGCCTTGGTTACTCGCCAAATTGCCACT
ATGTTAATGGCTGGCGTACCCTTAATTCAATCTATTGAAATGATAGCTTCTGGCTCAACAAATAAAAGCGTTACCAAGCT
TATCTCAGCTGTAGGCGATGATGTAAAAGCTGGTCAGCCTTTGTCTTTATCACTTAGAAAATATCCACGTTATTTTGATG
ACTTATATTGTGATTTAGTTGAATCTGGCGAGCAGTCAGGAGCACTTGATAAAATATTTGATCGCATAGCTATTTATAAA
GAAAAATCAGAAGCGCTTAAATCAAAAATTAAAAAAGCAATGTTTTACCCTATTGCTGTCTTAGTCATTGCCCTTATAGT
GACCTCAATTTTGCTTATTTTCGTAGTGCCACAATTTCAGGACATTTTTGCAGGGTTTGGGGCTGAACTCCCTGCATTTA
CGTTATTTGTAATTGGTATTTCAGATTTTATGCAAGAATCTTGGTGGATGGCTCTGATTGCGATAGTGGTTTTTGGCTGG
ACTTTTAAAGAGGCTAAATTAAGAAGCCTAAAGCTCAGGGATGCAACCGACAGAGCCATACTTAAGCTCCCTGTTATTGG
CATGATTTTAAACAAAGCGGCTGTGGCACGTTACGCGCGAACACTTTCGACCACATTTGCTGCCGGTGTTCCATTAGTTG
ATGCACTTGATTCAGCAGCAGGTGCTTCGGGCAATGCAGTGTACCGTTATGCTATTTTAGATATTAAAGCAGAGGTTAGC
TCAGGTAACCAAATGAACTGGGCAATGAGAAACTCAAAAATCTTCCCTGACATGGTAATCCAAATGGTTGCTATCGGTGA
AGAATCTGGCTCGCTCGATGGTATGTTAGCTAAGGTCGCGACTATTTATGAGCAAGAAGTAGACGATGCTGTAGATGGCT
TATCGAGCTTACTAGAGCCATTAATTATGGCTGTATTAGGTGTACTTGTTGGTGGTTTAATTGTTGCTATGTACCTCCCT
ATTTTCCAACTCGGTTCTGTTATTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(279-400)

Type II secretion system (T2SS), protein F (T2SSF)

(75-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

61.809

97.549

0.603
  pilC Acinetobacter baumannii D1279779

55.528

97.549

0.542
  pilC Legionella pneumophila strain ERS1305867

55.138

97.794

0.539
  pilC Acinetobacter baylyi ADP1

53.96

99.02

0.534
  pilC Vibrio cholerae strain A1552

44.389

98.284

0.436
  pilG Neisseria meningitidis 44/76-A

43.333

95.588

0.414
  pilG Neisseria gonorrhoeae MS11

43.077

95.588

0.412
  pilC Vibrio campbellii strain DS40M4

41.337

99.02

0.409

Multiple sequence alignment