Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AOR04_RS15335 Genome accession   NZ_CP012737
Coordinates   3411759..3413435 (+) Length   558 a.a.
NCBI ID   WP_096793821.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. 1_2015MBL_MicDiv isolate 15DKN1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3406759..3418435
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOR04_RS15310 (AOR04_15130) nadC 3406880..3407725 (+) 846 WP_096794146.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AOR04_RS21900 (AOR04_15135) - 3408186..3408644 (+) 459 WP_269467059.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AOR04_RS15320 (AOR04_15140) pilC 3408705..3409931 (+) 1227 WP_096793818.1 type II secretion system F family protein Machinery gene
  AOR04_RS15325 (AOR04_15145) pilD 3409947..3410831 (+) 885 WP_096793819.1 A24 family peptidase Machinery gene
  AOR04_RS15330 (AOR04_15150) coaE 3410833..3411480 (+) 648 WP_096793820.1 dephospho-CoA kinase -
  AOR04_RS15335 (AOR04_15155) pilB 3411759..3413435 (+) 1677 WP_096793821.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AOR04_RS15340 (AOR04_15160) yacG 3413552..3413782 (+) 231 WP_007376049.1 DNA gyrase inhibitor YacG -
  AOR04_RS15345 (AOR04_15165) - 3414065..3414553 (-) 489 WP_096793822.1 Spy/CpxP family protein refolding chaperone -
  AOR04_RS15350 (AOR04_15170) - 3414743..3415432 (+) 690 WP_008136799.1 response regulator -
  AOR04_RS15355 (AOR04_15175) - 3415410..3416825 (+) 1416 WP_096793823.1 ATP-binding protein -
  AOR04_RS15360 (AOR04_15180) - 3416829..3417775 (+) 947 Protein_2933 alpha/beta hydrolase -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62240.62 Da        Isoelectric Point: 5.4781

>NTDB_id=157412 AOR04_RS15335 WP_096793821.1 3411759..3413435(+) (pilB) [Pseudoalteromonas sp. 1_2015MBL_MicDiv isolate 15DKN1]
MNINSPLLRKFITLGRIDADTVKVKQRDFASTAELIAKCGNIDSKDLAEQCIDLFRVPYFDLKDFEPARIPAELVKEKLI
RKHHILPLVQKGRKVYIAASDPTDYGAFENYEFSTGLSCEIVVVDYIELDKKIDQLLDATGSLNLSDDEFKEFADLDSEN
TKEATPQDDEKDDAPIIVYINKILMDAIKKGASDLHFEPYEHKYRIRFRIDGILHEVASPPNTLATKLSARIKVMSRLDI
AEKRKPQDGRIKLKITERKSIDFRVSTMPTLWGEKIVMRILDSSSAMLGIDVLGYEPEQKKLYMDALAQPQGMILVTGPT
GSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGINQVQINIKADMTFANALRAFLRQDPDVVMVGEIRDLETAEISIK
AAQTGHLVLSTLHTNSAPETITRLLNMGVPAYNVASSISLIIAQRLARRLCPKCKTPEILPNEELTRQGFSEQQISEMTL
YAPKGCDNCTDGYKGRVGIYEVMQITPEIAQIIMRGGNSLEIAEVSLKAGFNNLRLSGIRKAADGMTSLAEINRVTSF

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=157412 AOR04_RS15335 WP_096793821.1 3411759..3413435(+) (pilB) [Pseudoalteromonas sp. 1_2015MBL_MicDiv isolate 15DKN1]
ATGAATATTAACTCACCGTTACTAAGAAAATTTATTACCCTTGGCCGTATTGATGCAGATACCGTTAAAGTAAAACAACG
AGATTTTGCCTCAACCGCCGAGCTTATTGCCAAATGTGGAAACATCGATAGCAAAGATTTAGCTGAGCAATGTATTGATT
TATTTCGCGTACCTTATTTCGATTTAAAAGACTTTGAACCTGCAAGGATCCCTGCCGAACTTGTAAAAGAAAAGCTGATC
CGTAAACACCATATTTTACCCTTGGTGCAAAAAGGTAGAAAAGTATACATAGCCGCGTCTGATCCGACCGATTATGGTGC
GTTTGAAAACTATGAATTTAGTACCGGCTTATCCTGTGAAATAGTAGTAGTTGACTACATTGAACTTGATAAAAAAATTG
ACCAATTGCTTGATGCAACTGGTAGCTTAAATTTAAGCGATGACGAATTTAAAGAATTTGCTGACTTAGATAGTGAAAAC
ACTAAAGAAGCCACCCCACAAGATGATGAAAAAGATGATGCACCTATCATTGTTTACATCAATAAAATCCTAATGGATGC
CATTAAAAAAGGCGCCTCTGATTTACACTTTGAACCTTATGAGCATAAGTACCGCATCCGTTTTCGTATTGATGGTATTT
TACATGAGGTAGCCAGCCCACCTAATACCTTAGCAACAAAACTTTCTGCACGAATAAAAGTAATGTCACGCCTAGATATT
GCCGAAAAACGTAAACCACAAGATGGTCGTATAAAGCTAAAAATTACTGAACGCAAAAGTATCGATTTTCGTGTAAGTAC
AATGCCGACCCTGTGGGGGGAAAAAATAGTAATGCGTATTCTTGATTCATCAAGCGCAATGCTAGGTATTGATGTATTAG
GCTACGAGCCGGAACAGAAAAAACTCTATATGGATGCGCTTGCTCAGCCTCAAGGGATGATTTTAGTTACAGGACCAACG
GGCTCGGGTAAAACAGTATCACTTTACACTGGTTTAAATATACTTAATCAACCAGAGCGTAATATTAGTACCGCAGAAGA
TCCTGTTGAAATTAATCTCGAAGGTATAAACCAAGTGCAAATTAATATTAAAGCCGATATGACCTTTGCAAATGCATTAC
GTGCATTTTTACGCCAAGATCCTGATGTCGTCATGGTAGGTGAAATACGTGACCTCGAAACAGCTGAAATATCAATAAAA
GCGGCGCAAACAGGTCACTTAGTACTATCAACTCTGCATACTAATTCAGCACCTGAAACGATAACTCGGTTATTAAATAT
GGGAGTACCAGCTTATAACGTAGCAAGTTCAATTAGTTTGATTATAGCCCAGCGCTTGGCTCGACGTTTATGCCCTAAAT
GTAAAACACCGGAAATATTACCAAACGAAGAACTTACCCGCCAAGGTTTTAGCGAGCAACAAATTAGTGAAATGACCCTC
TATGCACCTAAAGGATGTGATAATTGCACTGATGGTTATAAGGGTCGTGTCGGTATTTATGAAGTAATGCAAATAACACC
AGAGATAGCACAAATTATAATGCGTGGCGGTAATTCATTAGAAATAGCAGAAGTATCACTTAAAGCAGGATTTAATAACT
TGCGATTATCAGGTATTCGCAAAGCGGCTGATGGAATGACTTCGCTTGCTGAAATTAATCGAGTAACAAGTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(182-449)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.649

91.935

0.548
  pilB Acinetobacter baylyi ADP1

58.416

90.502

0.529
  pilB Legionella pneumophila strain ERS1305867

53.471

95.52

0.511
  pilB Vibrio cholerae strain A1552

52.142

96.237

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

52.247

95.699

0.5
  pilB Vibrio campbellii strain DS40M4

51.963

95.878

0.498
  pilF Neisseria gonorrhoeae MS11

49.808

93.548

0.466

Multiple sequence alignment