Detailed information NTG0157411

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AOR04_RS15325
• Genome accession: NZ_CP012737
• Coordinates: 3409947..3410831 (+)
• Length: 294 a.a.
• NCBI ID: WP_096793819.1
• Uniprot ID: -
• Organism: Pseudoalteromonas sp. 1_2015MBL_MicDiv isolate 15DKN1
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3404947..3415831

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AOR04_RS15305 (AOR04_15125)	ampD	3405551..3406105 (-)	555	WP_096793817.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
AOR04_RS15310 (AOR04_15130)	nadC	3406880..3407725 (+)	846	WP_096794146.1	carboxylating nicotinate-nucleotide diphosphorylase	-
AOR04_RS21900 (AOR04_15135)	-	3408186..3408644 (+)	459	WP_269467059.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
AOR04_RS15320 (AOR04_15140)	pilC	3408705..3409931 (+)	1227	WP_096793818.1	type II secretion system F family protein	Machinery gene
AOR04_RS15325 (AOR04_15145)	pilD	3409947..3410831 (+)	885	WP_096793819.1	A24 family peptidase	Machinery gene
AOR04_RS15330 (AOR04_15150)	coaE	3410833..3411480 (+)	648	WP_096793820.1	dephospho-CoA kinase	-
AOR04_RS15335 (AOR04_15155)	pilB	3411759..3413435 (+)	1677	WP_096793821.1	type IV-A pilus assembly ATPase PilB	Machinery gene
AOR04_RS15340 (AOR04_15160)	yacG	3413552..3413782 (+)	231	WP_007376049.1	DNA gyrase inhibitor YacG	-
AOR04_RS15345 (AOR04_15165)	-	3414065..3414553 (-)	489	WP_096793822.1	Spy/CpxP family protein refolding chaperone	-
AOR04_RS15350 (AOR04_15170)	-	3414743..3415432 (+)	690	WP_008136799.1	response regulator	-

Sequence

Protein

• Length: 294 a.a.
• Molecular weight: 32699.07 Da
• Isoelectric Point: 6.8611

>NTDB_id=157411 AOR04_RS15325 WP_096793819.1 3409947..3410831(+) (pilD) [Pseudoalteromonas sp. 1_2015MBL_MicDiv isolate 15DKN1]
MQSIVEVMQNELWFYLTTVGLVSLCVGSFLNVVIYRLPLMMQKEWQSECRILLADELKNSKAINNEQFNLVKPNSTCPKC
KTAIKPWQNIPVISWLFLKGKCASCNNPISIRYPVVEAITAILSLFIAFTMGANEQALLYILITWALVALTFIDIDHMLL
PDQLTLPLVWLALIASIMGYTVAPSDAIMGAVFGYLSLWSVFWLFKLLTGKEGMGYGDFKLLAVFGALLGWQSLLSIILL
SSIVGAVIGIVLLSIQGKDKATPIPFGPYLAIAGWITMLWGTELQNIYFNLIGY

Nucleotide

• Length: 885 bp

>NTDB_id=157411 AOR04_RS15325 WP_096793819.1 3409947..3410831(+) (pilD) [Pseudoalteromonas sp. 1_2015MBL_MicDiv isolate 15DKN1]
ATGCAAAGTATTGTAGAAGTAATGCAAAATGAGCTGTGGTTTTATCTAACCACCGTTGGCTTGGTTAGTTTATGTGTGGG
CAGTTTTTTAAATGTAGTGATTTATCGCCTACCACTTATGATGCAAAAAGAATGGCAAAGTGAGTGTAGAATACTGTTAG
CAGACGAACTTAAAAATAGTAAAGCAATAAATAATGAGCAATTTAATTTAGTAAAGCCGAACTCAACCTGCCCAAAGTGC
AAAACAGCTATTAAACCTTGGCAAAATATTCCTGTTATTAGCTGGTTATTTTTAAAAGGAAAATGTGCAAGTTGTAATAA
CCCTATATCAATACGCTATCCTGTAGTCGAAGCCATTACAGCAATATTGAGCTTATTTATTGCTTTTACTATGGGCGCAA
ATGAACAAGCTTTACTGTATATATTGATTACTTGGGCATTAGTTGCACTAACCTTTATTGATATAGACCATATGCTATTA
CCCGATCAACTTACATTACCACTAGTATGGCTTGCTTTAATAGCGTCAATAATGGGCTATACCGTAGCGCCGAGCGATGC
AATTATGGGTGCGGTTTTTGGTTATTTAAGCCTATGGAGTGTATTTTGGCTATTTAAGTTACTAACGGGCAAAGAAGGCA
TGGGCTATGGCGACTTTAAATTACTCGCTGTATTTGGTGCATTACTTGGTTGGCAATCACTACTTAGCATTATATTACTT
TCAAGTATTGTTGGCGCTGTTATTGGCATTGTATTACTCAGTATTCAAGGAAAAGACAAAGCAACCCCGATTCCATTTGG
TCCTTACCTTGCCATTGCAGGCTGGATCACTATGCTATGGGGCACTGAATTACAAAACATTTATTTTAATTTAATTGGTT
ATTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (142-250)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (22-130)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	51.724	98.639	0.51
pilD	Acinetobacter baumannii D1279779	50.345	98.639	0.497
pilD	Vibrio cholerae strain A1552	51.957	95.578	0.497
pilD	Acinetobacter nosocomialis M2	50	98.639	0.493
pilD	Neisseria gonorrhoeae MS11	46.792	90.136	0.422